华南8号木薯及其四倍体诱导株系叶片蛋白质组及叶绿素荧光差异分析

doi:10.3864/j.issn.0578-1752.2013.19.003

Abstract

Abstract: 【Objective】An investigation aimed at a better understanding of the differences in leaves proteins and its relationship with photosynthetic efficiency of cassava diploid and its tetraploid mutants, leaves proteins and chlorophyll fluorescence parameters were analyzed.【Method】Standard chromosome counting and flow cytometric analyses were used to determine the chromosome numbers and ploidy of each taxon, leaves proteins of cassava tetraploid mutants and diploid genotypes were excised and purified from 2D gels. Delta 2D software were used to analyze the differential proteins and MALDI-TOF-TOF-MS/MS were used to identify it. And western blot was used to verify some differential expressed proteins. 95% ethanol extraction method was used to measure the chlorophyll content and imaging-pam was used to determine the fluorescence parameters.【Result】Induced SC8 polyploid mutants were tetraploid, 13 differential expressed protein spots were identified, in which 12 were up-regulated and 1 was down-regulated, and 12 were successfully matched in database. The function of these 12 proteins which up-regulated were associated with carbohydrate and energy metabolism, photosynthesis, antioxidant and protein metabolism, 1 down-regulated protein was not matched in database, in which 46.2% differential expressed proteins were related with photosynthesis, chlorophyll a, chlorophyll b and total chlorophyll in tetraploid mutants were significantly increased, also the fluorescence parameters including Fo, ΦPSII, qP, NPQ and ETR.【Conclusion】Protein expression levels related with carbohydrate and energy metabolism and photosynthesis were up-regulated, chlorophyll content and chlorophyll fluorescence parameters were increased significantly, these indicate that high light-harvesting ability and high photochemical conversion rate in PSII reaction center of cassava tetraploid mutant leaves, then improving photosynthesis efficiency of SC8 tetraploidy mutants.

Key words: Cassava , tetraploid mutants , differential proteins , photosynthetic efficiency

AN Fei-Fei-1, FAN Jie-1, LI Geng-Hu-2, JIAN Chun-Ping-2, LI Kai-Mian-1. Comparison of Leaves Proteome and Chlorophyll Fluorescence of Cassava cv. SC8 and Its Tetraploid Mutants[J].Scientia Agricultura Sinica, 2013, 46(19): 3978-3987.

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Comparison of Leaves Proteome and Chlorophyll Fluorescence of Cassava cv. SC8 and Its Tetraploid Mutants

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