Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (18): 3743-3749.doi: 10.3864/j.issn.0578-1752.2013.18.001

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Construction and Analysis of Normalized Full-Length cDNA Library of Upland Cotton Anthers

 MA  Jian-Hui-., LIU  Ji-., FAN  Shu-Li, SONG  Mei-Zhen, PANG  Chao-You, WEI  Heng-Ling, YU  Shu-Xun   

  1. 1.Institute of Cotton Research of CAAS/State Key Laboratory of Cotton Biology, Anyang 455000, Henan
    2.College of Agronomy, Northwest A & F University, Yangling 712100, Shaanxi
  • Received:2013-03-01 Online:2013-09-15 Published:2013-06-25

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Abstract: 【Objective】The objective of this study is to understand the anther development and make a firm foundation for sterile mechanism in upland cotton (Gossypium hirsutum L.). 【Method】A normalized full-length cDNA library from anthers including the full development stage was firstly constructed and 9 896 high-quality ESTs were sequenced in this study. The bioinformatics analysis and qRT-PCR were performed to deep analyze this cDNA library. 【Result】The storage capacity of this cDNA library is 1.2×107 and the average length of the inserted cDNA fragment was dispersed between 1 kb and 3 kb. These high-quality ESTs were further assembled into 6 643 unigenes with 779 bp in average length. Through bioinformatics analysis of these unigenes, many important biological processes were obtained during the anther development and 28 genes related with the biosynthesis of pollen wall were identified during anther development. The qRT-PCR was performed to check the expression pattern of these pollen wall biosynthesis related genes and all of them were high-expressed in early anther development especially in tetrad pollen period. 【Conclution】In this study, lots of anther expressed genes were firstly sequenced in upland cotton. Many important processes regulating anther development were obtained and many genes related with pollen wall biosynthesis were obtained.

Key words: Gossypium hirsutum L. , cDNA library , anther , pollen wall

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