Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (9): 1756-1761.doi: 10.3864/j.issn.0578-1752.2013.09.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Mechanism Analysis of AtBT4 from Arabidopsis thaliana Against Botrytis cinerea

 HAO  Cong-Cong, YANG  Ping, CHEN  Zhan, JIA  Jiao, ZHAO  Bin, SI  He-Long, HAN  Jian-Min, XING  Ji-Hong, DONG  Jin-Gao   

  1. 1.College of Life Science, Agricultural University of Hebei/Mycotoxin and Molecular Plant Pathology Laboratory, Baoding 071001, Hebei
    2.Institute of Fruit Tree, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050061
    3.Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Gongzhuling 050061, Jilin
  • Received:2012-12-30 Online:2013-05-01 Published:2013-03-18

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Abstract: 【Objective】The objective of this study is to reveal the interactions between AtBT4 gene and SA, JA signal pathways in Arabidopsis thaliana resistance to Botrytis cinerea.【Method】RT-PCR technology was used to analyze the expression of AtBT4 in Col-0 treated with SA, its analogues BTH, JA, ACC and B. cinerea, the expression of AtBT4 in various signals pathway mutants following SA or JA treatment and the expression of resistance-related genes in Col-0, bt4 and AtBT4 complemented plants. 【Result】The expression of AtBT4 in Col-0 treated with JA and B. cinerea was significantly enhanced. But no significant difference was observed in AtBT4 expression between the JA-insensitive jar1 mutants treated for 0 and 12 h with JA. The AtBT4 expression was significantly down-regulated in the eds5, sid2, and npr1 mutants compared with the Col-0 plants. Compared with the Col-0 and AtBT4 complemented plants, the expression of PR1, PR4, PDF1.2 and BIK1 genes was significantly down-regulated in the bt4 mutants. 【Conclusion】 The AtBT4 gene expression is induced by JA and B. cinerea and the AtBT4 gene deficiency alters expression of resistance-related genes, PR1, PR4, PDF1.2 and BIK1, suggesting the AtBT4 gene may be involved in the regulation of SA and JA singnal pathway to impact the resistance to B. cinerea in A. thaliana.

Key words: AtBT4 , Arabidopsis thaliana , Botrytis cinerea , disease-resistant pathway

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