Scientia Agricultura Sinica ›› 2016, Vol. 49 ›› Issue (15): 2879-2897.doi: 10.3864/j.issn.0578-1752.2016.15.003

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genome-Wide Expression Analysis of Glucosinolate Biosynthetic Genes in Arabidopsis Across Diverse Tissues and Stresses Induction

DU Hai, RAN Feng, LIU Jing, WEN Jing, MA Shan-shan, KE Yun-zhuo, SUN Li-ping, LI Jia-na   

  1. College of Agronomy and Biotechnology, Southwest University, Chongqing 400715
  • Received:2016-03-09 Online:2016-08-01 Published:2016-08-01

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Abstract: 【Objective】The aim of this study is to analyze the expression profiles of Arabidopsis glucosinolate biosynthetic genes in different tissues at different developmental stages and under various stress treatments, so as to facilitate the identification of the key genes involved in controlling glucosinolate biosynthesis and provide valuable information for exploring the metabolic mechanism of glucosinolate biosynthesis as well as their functions in plant defenses. 【Method】 The dynamic expression profiles of 82 glucosinolate biosynthetic structural genes and regulating genes across a wide range of developmental stages and biotic and abiotic stresses were examined, based on ten expression microarray data and two RNA-Seq data in PLEXdb and AtGenExpress database. The expression patterns of ten key structural genes and regulating genes involved in glucosinolate biosynthesis pathway were further confirmed by Real-time PCR method. The correlation of the expression patterns of glucosinolate biosynthesis related genes was analyzed by STRING v10 software. 【Result】 The expressions of candidate genes between vegetative and reproductive organs were significantly different, with the majority of the candidate genes have higher expression levels in vegetative organs and lower expression levels in reproductive organs, indicating glucosinolates were mainly synthesized in vegetative tissues. The aliphatic glucosinolate biosynthesis genes were preferential expressed in shoot, such as stems and leaves, while indolic glucosinolate related genes were highly expressed in both of root and shoot, such as roots, stems and leaves. Moreover, the expressions of genes in a given tissue at different developmental stages were generally distinct, implying a temporal and spatial expression pattern. Real-time PCR analyses revealed that the expression profiles of the ten representive glucosinolate biosynthetic genes kept consistent with the results of microarray data assay. Furthermore, the expressions of aliphatic and indolic glucosinolate biosynthesis genes were tend to be induced by many bio- and abio-stresses, whereas indolic glucosinolate biosynthesis related genes were obviously induced by bio- and abio-stresses, such as Pseudomonas syringae, Alternaria brassicicola, Myzus persicae, low temperature (4℃), salt and high temperature (38℃). In addition, co-expression analysis revealed that the expressions of regulating genes were highly correlated to those of metabolic genes in aliphatic or indolic glucosinolate biosynthesis pathway. 【Conclusion】 The glucosinolates biosynthesis genes were preferentially expressed in vegetative tissues, as compared to seed tissues. There is a transport system that is responsible for transporting glucosinolates that were synthesized in vegetative tissues to seed tissues. The expressions of indolic glucosinolate biosynthesis genes tend to be induced by bio- and abio-stresses, indicating indolic glucosinolates are more important for Arabidopsis defense.

Key words: Arabidopsis thaliana, glucosinolates, expression patterns, development stage, biotic stresses, abiotic stresses, real- time PCR

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