Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (3): 630-638.doi: 10.3864/j.issn.0578-1752.2013.03.020

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

mRNA Expression Level and Promoter Methylation of DDX4 Gene in Testes of Yak and Cattle-Yak

 ZHOU  Yang, LUO  Hua, LI  Bo-Jiang, JIA  Chao, XIE  Zhuang, ZHAO  Xing-Bo, ZHONG  Jin-Cheng, LI  Qi-Fa   

  1. 1.College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095
    2.College of Animal Science and Technology, China Agricultural University, Beijing 100193
    3.College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041
  • Received:2012-07-09 Online:2013-02-01 Published:2012-08-15

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Abstract: 【Objective】The study was aimed to investigate the expression levels and promoter methylation status of DDX4 gene in the testes of yak and cattle-yak. 【Method】Real-time PCR was employed to examine the expression levels in the testes of yak and cattle-yak. Clone sequencing was applied to acquire the promoter sequences of DDX4 gene of yak. The promoter methylation status of DDX4 gene in the testes of yak and cattle-yak was analyzed by using sodium bisulfite sequencing.【Result】The mRNA expression level of DDX4 gene in the testis of yak was significantly higher than that of cattle-yak (P＜0.01). The length of the promoter sequences of the DDX4 gene was 1 370 bp, which included a core promoter (251 bp) and a CpG island (918 bp). The promoter methylation level of DDX4 gene in the testes of cattle-yaks (86.5%) was significantly higher than that in yak (67.0%) (P＜0.01). 【Conclusion】Results of the study showed that the testicular expression level of DDX4 might be involved in cattle-yak male sterility, and the promoter hypermethylation was correlated to the lower expression of DDX4 in the testes of cattle-yak.

Key words: yak , cattle-yak , DDX4 gene , expression level , methylation

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