川西北高原2009-2016年牦牛源大肠杆菌耐药性变迁和整合子携带分析

doi:10.3864/j.issn.0578-1752.2017.09.016

Abstract

Abstract: 【Objective】Aiming at the trend of drug resistance of Escherichia coli increased year by year and the problem of antibacterial agents for diseases control and prevention in yak (Bos grunniens), 1 908 Escherichia coli isolates from feces of free-ranging yaks (including the healthy and ill yaks), gastrointestinal contents (slaughtered yaks) and viscera samples (dead yaks) were chosen for antimicrobial susceptibility testing to 27 antibacterial agents and integrase genes detection between 2009 and 2016 in this study. The results of the study will be helpful to investigation of the vicissitude of drug resistance and integron-carrying of Escherichia coli isolated from yak and provide a reference for safe medication for yak in northwest Sichuan plateau.【Method】Broth microdilution method and WHONet5.6 software were used, respectively, for minimum inhibitory concentration (MIC) determination according to the guidelines of Clinical and Laboratory Standards Institute (NCCLS) and the experimental data statistical analysis. Class 1 and class 2 integrase genes were amplified using conventional PCR for the detection of integron-carrying of class 1 and class 2 integrons. 【Result】 The results showed that: the drug resistance level to 27 antibacterial agents of the strains isolated between 2009 and 2016 kept a rising trend year by year. Except that the drug resistance level to oxytetracycline of the strains isolated between 2014 and 2016 and the drug resistance level to sulfonamides of the strains isolated between 2015 and 2016 were over 60.00%, all the other strains isolated in different years revealed lower drug resistance level to other 23 antibacterial agents and the drug resistance level to mequindox and rifampicin were lower than 30.00%, and the drug resistance rate to mequindox was lower than rifampicin between 2009 and 2016. Integron-carrying rate of Escherichia coli isolated in different years was different, 774 isolates carried at least one type of integron, accounting for 41.30% (788/1908) and 140 isolates carried both class 1 and class 2 integron, accounting for 7.34% (140/1908). Of which, 582 isolates carried class 1 integron and 346 isolates carried class 2 integron, accounting for 30.50% (582/1908) and 18.13% (346/1908), respectively.【Conclusion】The results of vicissitude of drug resistance and integron-carrying of Escherichia coli isolated from yak between 2009 and 2016 in northwest Sichuan plateau could provide a theoretical basis and experimental data for epidemiology study and screening for preventive drugs and ensure the reasonable application of antibacterial drugs in northwest Sichuan plateau.

Key words: Northwest Sichuan plateau, yak, vicissitude of drug resistance, integrons

CHEN ChaoXi, HE DongMei, TANG Cheng. Vicissitude of Drug Resistance and Integron-Carrying of Escherichia coli Isolated from Yak Between 2009 and 2016 in Northwest Sichuan Plateau[J].Scientia Agricultura Sinica, 2017, 50(9): 1705-1713.

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Vicissitude of Drug Resistance and Integron-Carrying of Escherichia coli Isolated from Yak Between 2009 and 2016 in Northwest Sichuan Plateau

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