Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (3): 606-613.doi: 10.3864/j.issn.0578-1752.2013.03.017

• STORAGE·FRESH-KEEPING·PROCESSING • Previous Articles     Next Articles

Protective Effect of Lactobacillus plantarum C88 on H2O2-Induced Oxidative Stress in Caco-2 Cells

 LI  Sheng-Yu, LI  Da, ZHAO  Yu-Juan, ZHANG  Xue, HUANG  Li, ZHAO  Yu-Jian, YANG  Zhen-Nai   

  1. 1.Institute of Agro-food Technology, Jilin Academy of Agricultural Sciences/National R&D Branch Centre for Milk Processing, Changchun 130033
    2.School of Food Science, Beijing Technology and Business University, Beijing 100048
  • Received:2012-10-08 Online:2013-02-01 Published:2012-12-21

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Abstract: 【Objective】 The aim of the study was to evaluate the antioxidant activity of L. plantarum C88 against H2O2 induced Caco-2 cell oxidative stress.【Method】In vitro methods simulating gastric and small intestine digestive processes, coupled with human colon carcinoma cells in oxidative stress, to hydrogen peroxide at concentrations of 0.1 mmol•L-1 and 0.2 mmol•L-1 for 12 h, 24 h, 36 h, 48 h, are the valid tools to evaluate the antioxidant activity of L. plantarum C88. The free radical scavenging capacity and antioxidant enzymes activitties in the cell culture supernatants and cell disruptions were measured.【Result】Compared with the oxidative stress group, L. plantarum C88 at 1011CFU/mL signi?cantly increased the glutathione peroxidase (GSH-Px) (P＜0.05) and the total antioxidation capacity (T-AOC) (P＜0.05) in the cultured supernatants, the hydroxyl radical scavenging ability (P＜0.05), the glutathione peroxidase (GSH Px) (P＜0.05) and the superoxide dismutase (SOD) activity in the cell disruptions.【Conclusion】Results of the study suggested that L. plantarum C88 could protect the Caco-2 cells against H2O2-induced oxidative stress by renewing the enzymatic and non-enzymatic antioxidant defense system.

Key words: lactic acid bacteria , Lactobacillus plantarum , antioxidant activity , probiotic

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