Scientia Agricultura Sinica ›› 2017, Vol. 50 ›› Issue (5): 959-968.doi: 10.3864/j.issn.0578-1752.2017.05.019

• RESEARCH NOTES • Previous Articles    

Evaluation on Malolactic Fermentation Potential of Wine Lactobacillus plantarum

BU Xiao1, XUE Xue2, CHENG Jing3, LIU ShuWen2,4,5   

  1. 1College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi; 2 College of Enology, Northwest A&F University, Yangling 712100, Shaanxi; 3 College of Food Science and Nutritional Engineering, China Agricultural University/ Center for Viticulture and Enology, Beijing 100083; 4Shaanxi Engineering Research Center for Viti-Viniculture, Yangling 712100, Shaanxi; 5Viti-viniculture Experiment Station (Heyang) of Northwest A&F University, Weinan 715300, Shaanxi
  • Received:2016-06-20 Online:2017-03-01 Published:2017-03-01

Abstract: 【Objective】For the development of wine Lactobacillus plantarum as potential commercial malolactic fermentation (MLF) starter cultures, the process of MLF in Cabernet Sauvignon wine by L. plantarum was investigated in this study, to evaluate the MLF potential of L. plantarum.【Method】This research used L. plantarum CS-1, XJ-14, XJ-25 and XJA-2 (isolated from Xinjiang wine in authors’ lab with high glycosidase activity) as the research objects, and MLF in Cabernet Sauvignon wine, respectively, wine without MLF was set as the control group. The growth of strains, changes of malic acid content through the MLF, and the differences of wine aroma compositions before and after MLF were compared. Through analysis of the above three indicators to evaluate the MLF potential of L. plantarum more comprehensively.【Result】During the first 6 days of the MLF process, the density of 4 strains of L. plantarum decreased significantly, while the trend was began to slow down after inoculation for 6 days. All the strains showed a good deacidification ability. The deacidification ability of strain XJA-2 was slightly higher than other strains, and the concentration of malic acid in wine was decreased from 2.3 g·L-1 to 1.0 g·L-1 in 15 days, but all the strains failed to complete the MLF. Strain XJ-25 treatment could significantly reduce the unpleasant aroma of green and bring more rich floral and fruity, strainXJ-14 could also reduce the green flavor of wine, but the floral aroma was relatively weaker than strain XJ-25, while strain CS-1 and strain XJA-2 slightly reduced the green flavor of wine, but due to the increase of chemical and vegetal flavor, the floral and fruity of wine was covered.【Conclusion】Using L. plantarum XJ-25 to start MLF could release the alcohol ester aroma substances more easily, to enhance the fruit and floral characteristics of wine. This strain was more advantageous to improve the quality of wine aroma compared with using other three L. plantarum strains. Therefore, L. plantarum XJ-25 has the potential of development of commercial starter cultures.

Key words: Lactobacillus plantarum, malolactic fermentation, wine, deacidification, aroma

[1]    GENISHEVA Z, MOTA A, MUSSATTO S I, OLIVEIRA J M, TEIXEIRA J A. Integrated continuous winemaking process involving sequential alcoholic and malolactic fermentations with immobilized cells. Process Biochemistry, 2014, 49(1): 1-9.
[2]    GONZALEZ L, SANTAMARIA P, LOPEZ R, LOPEZ-ALFARO I. Indigenous lactic acid bacteria communities in alcoholic and malolactic fermentations of tempranillo wines elaborated in ten wineries of La Rioja (Spain). Food Research International, 2013, 50(1): 438-445.
[3]    LIU S Q. Malolactic fermentation in wine-Beyond deacidification. Journal of Applied Microbiology, 2002, 92(4): 589-601.
[4]    BARTOWSKY E J, BORNEMAN A R. Genomic variations of Oenococcus oeni strains and the potential to impact on malolactic fermentation and aroma compounds in wine. Applied Microbiology & Biotechnology, 2011, 92(3): 441-447.
[5]    KNOLL C, FRITSCH S, SCHNELL S, GROSSMANN M, RAUHUT D, TOIT M. Influence of pH and ethanol on malolactic fermentation and volatile aroma compound composition in white wines. LWT-Food Science and Technology, 2012, 44(10): 2077-2086.
[6]    BAFFI M A, TOBAL T, HENRIQUE J, LAGO G, LEITE R, BOSCOLO M, GOMES E, DA-SILVA R. A novel β-glucosidase from Sporidiobolus pararoseus: characterization and application in winemaking. Journal of Food Science, 2011, 76(7): C997-C1002.
[7]    BAFFI M A, MARTIN N, TOBAL T M, FERRAREZI A, LAGO J, BOSCOLO M, GOMES E, SILVA R. Purification and characterization of an ethanol-tolerant β-glucosidase from Sporidiobolus pararoseus and its potential for hydrolysis of wine aroma precursors. Applied Biochemistry & Biotechnology, 2013, 171(7): 1681-1691.
[8]    彭传涛, 贾春雨, 文彦, 陶永胜. 苹果酸-乳酸发酵对干红葡萄酒感官质量的影响. 中国食品学报, 2014, 14(2): 261-268.
PENG C T, JIA C Y, WEN Y, TAO Y S. Influence of malo-lactic fermentation on the sensory quality of dry red wine. Journal of Chinese Institute of Food Science and Technology, 2014, 14(2): 261-268. (in Chinese)
[9]    LONVAUD A. Lactic acid bacteria in the quality improvement and depreciation of wine. Antonie Van Leeuwenhoek, 1999, 76(1/4): 317-331.
[10]   RODRIGUEZ J M, VILA J. Immobilization of Oenococcus oeni in Lentikats®; To develop malolactic fermentation in wines. Biotechnology Progress, 2013, 29(1): 60-65.
[11]   SUMBY K M, JIRANEK V, GRBIN P R. Ester synthesis and hydrolysis in an aqueous environment, and strain specific changes during malolactic fermentation in wine with Oenococcus oeni. Food Chemistry, 2013, 141(3): 1673-1680.
[12]   LI N, DUAN J, GAO D, LUO J, ZHENG R, BIAN Y, ZHANG X, JI B. Mutation and selection of Oenococcus oeni for controlling wine malolactic fermentation. European Food Research & Technology, 2015, 240(1): 93-100.
[13]   LERM E, ENGELBRECHT L, TOITM D. Selection and characterisation of Oenococcus oeni and Lactobacillus plantarum south african wine isolates for use as malolactic fermentation starter cultures. South African Journal for Enology & Viticulture, 2011, 32(2): 280-295.
[14]   BRAVO B M, HOLLMANN A, DELFEDERICO L, DANAY V H, CABALLERO A, SEMORILE L. Patagonian red wines: Selection of Lactobacillus plantarum isolates as potential starter cultures for malolactic fermentation. World Journal of Microbiology & Biotechnology, 2013, 29(9): 1537-1549.
[15]   TOIT M D, ENGELBRECHT L, LERM E, SRIEGER-WEBER S. Lactobacillus: The next generation of malolactic fermentation starter cultures-an overview. Food & Bioprocess Technology, 2010, 4(6): 876-906.
[16]   但霞. 不同产区葡萄酒乳酸菌的分离与鉴定[D]. 杨凌: 西北农林科技大学, 2012.
DAN X. Isolation and identification of wine lactic acid bacteria from different regions [D]. Yangling: Northwest Agriculture and Forestry University, 2012. (in Chinese)
[17]   李爱霞. 植物乳杆菌苹果酸—乳酸发酵性能及其苹果酸—乳酸酶的研究[D]. 杨凌: 西北农林科技大学, 2014.
LI A X. Study on malolactic fermentation and malolactic enzyme of Lactobacillus plantarum[D]. Yangling: Northwest Agriculture and Forestry University, 2014. (in Chinese)
[18]   杨世玲. 酒酒球菌β-葡萄糖苷酶活性及其对葡萄酒香气的影响研究[D]. 杨凌: 西北农林科技大学, 2015.
YANG S L. Study on β-glycosidase activity of Oenococcus oeni and its effect on wine aroma [D]. Yangling: Northwest Agriculture and Forestry University, 2015. (in Chinese)
[19]   GUTH H. Quantitation and sensory studies of character impact odorants of different white wine varieties. Journal of Agricultural & Food Chemistry, 1997, 45(8): 3027-3032.
[20]   PEINADO R A, MORENO J, BUENO J E, MORENO J A, MAURICIO J C. Comparative study of aromatic compounds in two young white wines subjected to pre-fermentative cryomaceration. Food Chemistry, 2004, 84(4): 585-590.
[21]   CULLERE L, ESCUDARO A, JUAN CACHO A, FERREIRA V. Gas chromatography−olfactometry and chemical quantitative study of the aroma of six premium quality spanish aged red wines. Journal of Agricultural & Food Chemistry, 2004, 52(6): 1653-1660.
[22]   蔡建. 发酵前处理工艺对天山北麓‘赤霞珠’葡萄酒香气改良研究[D]. 北京: 中国农业大学, 2014.
CAI J. Study on aroma quality improvement for Cabernet Sauvignon wines based on pre-fermentation technology from north slope of Tianshan mountains [D]. Beijing: China Agriculture University, 2014. (in Chinese)
[23]   SANCHEZ-PALOMO E, GARCIA-CARPINTERO E G, ALONSO- VILLEGAS R, GONZALEZ-VINAZ M A. Characterization of aroma compounds of Verdejo white wines from the La Mancha region by odour activity values. Flavour & Fragrance Journal, 2010, 25(6): 456-462.
[24]   CAPONE S, TUFARIELLO M, SICILIANO P. Analytical characterisation of Negroamaro   red wines by “Aroma Wheels”. Food Chemistry, 2013, 141(3): 2906-2915.
[25]   FRANCO M, PEINADO R A, MEDINA M, MORENO J. Off-vine grape drying effect on volatile compounds and aromatic series in must from Pedro Ximénez grape variety. Journal of Agricultural & Food Chemistry, 2004, 52(12): 3905-3910.
[26]   PEINADO R A, MAURICIO J C, MORENO J. Aromatic series in sherry wines with gluconic acid subjected to different biological aging conditions by Saccharomyces cerevisiae, var. capensis. Food Chemistry, 2006, 94(2): 232-239.
[27]   AZNAR M, LOPEZ R, CACHO J F, FERREIRA V. Identification and quantification of impact odorants of aged red wines from Rioja. GC-olfactometry, quantitative GC-MS, and odor evaluation of HPLC fractions. Journal of Agricultural & Food Chemistry, 2001, 49(6): 2924-2929.
[28]   LI H, TAO Y S, WANG H, ZHANG L. Impact odorants of Chardonnay dry white wine from Changli County (China). European Food Research & Technology, 2008, 227(1): 287-292.
[29]   SELLI S, CANBAS A, CABAROGLU T, ERTEN H, GUNATA Z. Aroma components of cv. Muscat of Bornova wines and influence of skin contact treatment. Food Chemistry, 2006, 94(3): 319-326.
[30]   WEN Y Q, HE F, ZHU B Q, LAN Y B, PAN Q H, LI C Y, REEVES M J, WANG J. Free and glycosidically bound aroma compounds in cherry ( Prunus avium L.). Food Chemistry, 2014, 152(6): 29-36.
[31]   何志刚, 梁璋成, 任香芸, 林晓姿, 李维新, 陆东和. 植物乳杆菌R23在枇杷酒中生长及苹果酸乳酸发酵特性研究. 中国食品学报, 2011, 11(4): 36-41.
HE Z G, LIANG Z C, REN X Y, LIN X Z, LI W X, LU D H. Studies on growth and malolactic fermentation characteristics of Lactobacillus plantarum R23 in loquat wine. Journal of Chinese Institute of Food Science and Technology, 2011, 11(4): 36-41. (in Chinese)
[32]   李静, 樊明涛, 孙慧烨. 植物乳杆菌对猕猴桃酒降酸效果的研究. 食品工业科技, 2016(1): 165-169.
LI J, FAN M T, SUN H Y. Effect of Lactobacillus plantarum on the deacification of kiwifruit wine. Science and Technology of Food Industry, 2016(1): 165-169.(in Chinese)
[33]   SWIEGERS J H, BARTOWSKY E J, HENSCHKE P A, PRETORIUS I S. Yeast and bacterial modulation of wine aroma and flavour. Australian Journal of Grape & Wine Research, 2005, 11(2): 139-173.
[34]   周安玲, 米兰, 李霁昕, 蒋玉梅. 几株乳酸菌对干红葡萄酒挥发性成分的影响. 食品工业科技, 2015, 36(15): 136-142.
ZHOU A L, MI L, LI J X, JIANG Y M. Impact of several strains of lactic acid bacteria on the volatile components of dry red wine. Science and Technology of Food Industry, 2015, 36(15): 136-142. (in Chinese)
[35]   FERREIRA V, LOPEZ R, CACHO J F. Quantitative determination of the odorants of young red wines from different grape varieties. Journal of the Science of Food & Agriculture, 2000, 80(11): 1659-1667.
[36]   HU K, QIN Y, Tao Y S, ZHU X L, PENG C T, ULLAH N. Potential of glycosidase from Non-Saccharomyces, isolates for enhancement of wine aroma. Journal of Food Science, 2016, 81(4): 935-943.
[37]   CARRAU F M, MEDINA K, BOIDO E, FARINA L, GAGGERO C, DEELACASSA E, VERSINI G, HENSCHKE P A. De novo synthesis of monoterpenes by Saccharomyces cerevisiae, wine yeasts. Fems Microbiology Letters, 2005, 243(1): 107-115.
[38]   MICHLMAYR H, NAUER S, BRANDES W, SCHUMANN C, KULBE K D, HIERRO A M, EDER R. Release of wine monoterpenes from natural precursors by glycosidases from Oenococcus oeni. Food Chemistry, 2012, 135(1): 80-87.
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