Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (2): 424-432.doi: 10.3864/j.issn.0578-1752.2013.02.022

• RESEARCH NOTES • Previous Articles     Next Articles

Isolation and Functional Analysis of the Stress Resistance Gene MsDUF in Medicago Sativa L.

 HAN  Bo, WANG  Wei-Dong, YANG  Pei-Zhi, ZHANG  Pan, HU  Tian-Ming   

  1. 1.College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi
    2.State Key Laboratory of Crop Stress Biology in Arid Areas, Yangling 712100,Shaanxi
  • Received:2012-09-29 Online:2013-01-15 Published:2012-11-26

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Abstract: 【Objective】This study is aimed to characterize the stress resistance gene MsDUF in Medicago sativa by cloning and analysis of its sequence, and study the function of MSDUF under stress conditions.【Method】The full-length cDNA sequence of MsDUF was isolated by RACE. The obtained cDNA sequence and the deduced amino acid sequence were analysed. Using gene gun in subcellular localization. Real-time quantitative PCR was used to assess the expression of MsDUF in response to NaCl，PEG-6000，ABA and GA3.【Result】The sequencing result showed that the cloned cDNA (designated as MsDUF, GenBank accession No. JX183734) was 714 bp, contains an open reading frame (ORF) of 633bp, and encoding a protein with 210 amino acids. BLASTp search revealed 53% to 98% similarities between the MSDUF protein and those reported MSDUF from other species, such as Arabidopsis thaliana, Glycine max and M. truncatula. The RT-PCR result showed that MsDUF was upregulatedly in response to stress resistance.【Conclusion】The stress resistance related gene MsDUF was cloned from M. sativa and found that it localized in cytoplasm. RT-PCR analysis showed that the gene MsDUF plays an important role in response to the abiotic stress. It is a novel stress resistance gene in M. sativa.

Key words: Medicago sativa , MsDUF , hypertonic stress , hormone-induced , subcellular localization , functional analysis

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