Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (21): 4457-4464.doi: 10.3864/j.issn.0578-1752.2012.21.014

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Study on Optimization of Non-Starch Polysaccharide Enzymes of Broiler Diets in Vitro

 HE  Ke-Lin, SA  Ren-Na, GAO  Jie, LI  Dong-Wei, ZHUANG  Xiao-Feng, ZHANG  Hong-Fu   

  1. 1.中国农业科学院北京畜牧兽医研究所/动物营养学国家重点实验室，北京 100193
  • Received:2012-03-22 Online:2012-11-01 Published:2012-05-08

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Abstract: 【Objective】The study was conducted to discuss the effects of non-starch polysaccharides (NSP) enzymes on the in vitro digestive energy (IVDE) of broiler diets of different contents of NSP.【Method】The first step was to investigate the dose-response of the NSP enzymes on the IVDE in diets using one-way randomized experimental design. Five NSP enzymes including xylanase, β-glucanase, cellulose, pectinase, and β-mannanase were added into corn-soybean diet and wheat-soybean diet, from 0 to 900 µg•g-1. The second step was to screen the optimum enzyme combinations using quadratic regress-orthogonal rotary design. The method of testing IVDE was a two-stage enzymic hydrolysis with pepsin-pancreatin in vitro.【Result】The IVDE of broiler diet regulating by NSP enzyme appeared S state diagram. The xylanase had more promotion effects than other NSP enzymes. It increased IVDE by 0.24 MJ•kg-1 and 0.40 MJ•kg-1 in corn-soybean diet and wheat-soybean diet, respectively. In corn-soybean diet, the optimum enzyme combinations were xylanase 34 836.4 U•kg-1, β-glucanase 6 762.0 U•kg-1, cellulose 1 159.2 U•kg-1, pectase 872.6 U•kg-1, and β-mannanase 24 535.9 U•kg-1. In wheat-soybean diet, the optimum enzyme combinations were xylanase 65 405.5 U•kg-1, β-glucanase 10 131.5 U•kg-1, cellulose 980.0 U•kg-1, pectase 501.6 U•kg-1, and β-mannanase 5 141.4 U•kg-1. 【Conclusion】The findings showed that using five kinds of NSP enzymes in corn-soybean and wheat-soybean diets of broiler could improve the in vitro digestive energy.

Key words: NSP enzyme, in vitro digestive energy, enzyme combination, broiler, in vitro

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