Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (19): 4032-4039.doi: 10.3864/j.issn.0578-1752.2012.19.015

• HORTICULTURE • Previous Articles     Next Articles

Analysis of the Specificity of Rootstock and Scion Combinations of ‘Hamlin’ Sweet Orange Related to Fruit Oleocellosis by FTIR

 ZHENG  Yong-Qiang, LIU  Yan-Mei, HE  Shao-Lan, YI  Shi-Lai, DENG  Lie, ZHOU  Zhi-Qin, JIAN  Shui-Xian, LI  Song-Wei   

  1. 1.西南大学-中国农业科学院柑橘研究所/国家柑橘工程技术研究中心，重庆 400712
    2.西南大学园艺园林学院/南方山地果树学教育部重点实验室，重庆 400715
    3.重庆市北碚区经济作物技术推广站，重庆 400700
  • Received:2012-05-09 Online:2012-10-01 Published:2012-08-23

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Abstract: 【Objective】Fourier transform infrared (FTIR) spectrometry, which is accurate, simple and efficient, with high resolution, was used to investigate the molecular physiological mechanisms of the specificity of rootstock and scion combinations of ‘Hamlin’ sweet orange [Citrus sinensis (L.) Osbeck cv. Hamlin] related to on-tree oleocellosis.【Method】The influence of ‘Hamlin’ sweet orange on Goutouchen sour orange [C. aurantium (L.). GT], Rich trifoliate 16-6 [P. trifoliate (L.). LC] and Cleopatra mandarin [C. reticulate (L.). IO] on rates of oleocellosis (RO) and degree of oleocellosis (DO) were measured at harvest stage. And the leaf and fruit peel FTIR spectroscopy of ‘Hamlin’ sweet orange on GT, LC and IO were also determined at the sensitive period of on-tree oleocellosis.【Result】The results showed that rootstocks had a significant influence on RO and DO of ‘Hamlin’ sweet orange fruits, and the fruits of ‘Hamlin’ sweet orange on GT and LC had the highest DO and RO, respectively. In contrast, the fruits of ‘Hamlin’ sweet orange on IO had the lowest DO and second higher RO. And the leaf absorption peak intensity (API) at 3420, 2927, 1625 and 1069 cm-1 of ‘Hamlin’ sweet orange on GT, IO and LC decreased in order. The above results showed that the influence of high temperature on leaf carbohydrate synthesis and transport, cell walls composition, protein stability and leaf membrane lipid peroxidation balance of ‘Hamlin’ sweet orange on GT were minimal, next was Hamlin sweet orange on IO, and ‘Hamlin’ sweet orange on LC were maximum. However, the API of peel FTIR of ‘Hamlin’ sweet orange on IO and GT was significantly lower than that of ‘Hamlin’ sweet orange on LC. Whilst, the changes of RO and DO were consistent with leaf FTIR and fruit peel FTIR, respectively.【Conclusion】 RO may be related to the response of citrus leaves to high temperature stress, in contrast, DO is directly related to fruit peel metabolism. And it is feasible to predict RO and DO by leaves and peels FTIR analysis, respectively. At the same time, leaf and peel FTIR analysis is helpful to reveal the stock-scion specific molecular and physiological mechanisms of on-tree oleocellosis.

Key words: Hamlin sweet orange, rootstock, oleocellosis, specificity between rootstock and scion, Fourier transform infrared spectrometry (FTIR)

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