基于SSR标记的甜瓜品种（系）DNA指纹图谱库的构建

doi:10.3864/j.issn.0578-1752.2012.13.012

Abstract

Abstract: 【Objective】 DNA fingerprinting database of varieties of melon (Cucumis melo L.) was constructed and the identification method was obtained. 【Method】The simple sequence repeat (SSR) primers were selected using 20 representative melon varieties (lines) for establishing fingerprints of 105 different varieties (lines). 【Result】Out of the 1 219 pairs of SSR primers, 18 pairs were screened to generate polymorphic fingerprints among 105 varieties or inbred lines. The results showed that each pair of the primers could detect 4-14 polymorphic bands with an average of 9. The average polymorphism information content (PIC) was 0.68 with a range of 0.55-0.82. The genetic similarity coefficient of the 105 accessions ranged from 0.70 to 0.99. The fingerprint data of all the 18 pairs of core SSR primers were employed to establish the fingerprinting database to distinguish the 105 samples and every accession had its unique fingerprinting. 【Conclusion】 The results indicated that SSR marker is suitable to construction of DNA fingerprinting database of melon varieties and the database could provide reference for melon identification.

Key words: melon(Cucumis melo L.), fingerprinting, SSR marker, core primer

SONG Hai-Bin, CUI Xi-Bo, MA Hong-Yan, ZHU Zi-Cheng, LUAN Fei-Shi. Construction of DNA Fingerprint Database Based on SSR Marker for Varieties(Lines) of Cucumis melo L.[J].Scientia Agricultura Sinica, 2012, 45(13): 2676-2689.

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Construction of DNA Fingerprint Database Based on SSR Marker for Varieties(Lines) of Cucumis melo L.

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