优质面条商品小麦澳白麦相关品质基因的分子标记鉴定

doi:10.3864/j.issn.0578-1752.2012.18.001

Abstract

Abstract: 【Objective】A commercial noodle wheat from Australia, known as Australian Standard White in China due to its top noodle quality, was a mixture of several genotypes. Noodle quality related genes were identified for providing information of noodle quality improvement to wheat breeding in China. 【Method】A total of 36 selected lines from Australian Standard White population were analyzed with 38 functional markers including high molecular weight glutenin subunits (HMW-GS), low molecular weight glutenin subunits (LMW-GS), 1B/1R translocation, waxy protein subunits, polyphenol oxidase (PPO) activity, yellow pigment content, grain hardness and resistance to pre-harvest sprouting (PHS). 【Results】In the 36 lines, there were 13 genes with positive effects on noodle quality, among which non-1B/1R translocation type and HMW-GS gene Bx7 covered 100%, whereas HMW-GS gene By8, LMW-GS genes Glu-A3b and Glu-B3b, Wx-B1 null type, low PPO activity allele Ppo-D1a, low yellow pigment allele Psy-B1b, soft to medium grain hardness allele Pinb-D1a and PHS resistant gene Vp-1Bc had frequencies over 80%. Out of the 36 lines, 12 genotypes were found, among which genotype 2 predominated in the highest frequency up to 63.9%, which covered the most of refered positive genes such as HMW-GS genes Bx7 and By8, LMW-GS genes Glu-A3b and Glu-B3b, low PPO activity gene Ppo-D1a, low yellow pigment content gene Psy-B1b, soft to medium hardness gene Pinb-D1a, PHS resistant gene Vp-1Bc, non-1B/1R translocation and Wx-B1 null type, and had the least of refered negative genes such as high PPO activity gene Ppo-A1a and high yellow pigment content gene Psy-A1a. 【Conclusion】Australian Standard White was a commercial wheat composed of several cultivars. Pyramiding most of positive noodle quality genes in cultivars and mixing the cultivars in the end-use quality needed proportion resulted in its high noodle quality. For a noodle wheat breeding plan, genotype 2 might be the most promising candidate of noodle quality donors in Australian Standard White.

Key words: Australian Standard White, noodle quality, quality gene, molecular marker

LI Shi-Zhao, WU Ling, ZHENG Jian-Min, ZHU Hua-Zhong. Molecular Marker Identification of Noodle Quality Related Genes in a Commercial Noodle Wheat from Australia[J].Scientia Agricultura Sinica, 2012, 45(18): 3677-3687.

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References

[1]张艳, 阎俊, Yoshida H, 王德森, 陈东升, Nagamine T, 刘建军, 何中虎. 中国面条的标准化实验室制作与评价方法研究. 麦类作物学报, 2007, 27(1): 158-165.

Zhang Y, Yan J, Yoshida H, Wang D S, Chen D S, Nagamine T, Liu J J, He Z H. Standardization of laboratory processing of Chinese white salted noodle and its sensory evaluation system. Journal of Triticeae Crops, 2007, 27(1): 158-165. (in Chinese)

[2]杨金, 张艳, 何中虎, 阎俊, 王德森, 刘建军, 王美芳. 小麦品质性状与面包和面条品质关系分析. 作物学报, 2004, 30(8): 739-744.

Yang J, Zhang Y, He Z H, Yan J, Wang D S, Liu J J, Wang M F. Association between wheat quality traits and performance of pan bread and dry white Chinese noodle. Acta Agronomica Sinica, 2004, 30(8): 739-744. (in Chinese)

[3]刘丽, 周阳, 何中虎, 王德森, 张艳, Peña R J. 高、低分子量麦谷蛋白亚基等位变异对小麦加工品质性状的影响. 中国农业科学, 2004, 37(1): 8-14.

Liu L, Zhou Y, He Z H, Wang D S, Zhang Y, Peña R J. Effect of allelic variation in HMW and LMW glutenin subunits on the processing quality in common wheat. Scientia Agricultura Sinica, 2004, 37(1): 8-14. (in Chinese)

[4]刘丽, 周阳, 刘建军, 何中虎, 杨金. Glu-1和Glu-3等位变异及1BL/1RS易位与面包和面条品质关系的研究. 中国农业科学, 2004, 37(9): 1265-1273.

Liu L, Zhou Y, Liu J J, He Z H, Yang J. Effect of allelic variation at the Glu-1 and Glu-3 loci and presence of 1BL/1RS translocation on pan bread and dry white Chinese noodle quality. Scientia Agricultura Sinica, 2004, 37(9): 1265-1273. (in Chinese)

[5]刘丽, 周阳, 何中虎, 阎俊, 张艳, Peña R J. Glu-1和Glu-3等位变异对小麦加工品质的影响. 作物学报, 2004, 30(10): 959-968.

Liu L, Zhou Y, He Z H, Yan J, Zhang Y, Peña R J. Effect of allelic variation at Glu-1 and Glu-3 loci on processing quality in common wheat. Acta Agronomica Sinica, 2004, 30(10): 959-968. (in Chinese)

[6]陈东升, 刘丽, 董建力, 何中虎, 张艳, 刘建军, 王德森. HMW-GS和LMW-GS组成及1BL/1RS易位对春小麦品质性状的影响. 作物学报, 2005, 31(4): 414-419.

Chen D S, Liu L, Dong J L, He Z H, Zhang Y, Liu J J, Wang D S. Effect of HMW and LMW glutenin subunits and presence of 1BL/1RS translocation on quality traits in spring wheat. Acta Agronomica Sinica, 2005, 31(4): 414-419. (in Chinese)

[7]刘丽, 阎俊, 张艳, 何中虎, Peña R J, 张立平. 冬播麦区Glu-1和Glu-3位点等位变异及1B/1R易位与小麦加工品质性状的关系. 中国农业科学, 2005, 38(10): 1944-1950.

Liu L, Yan J, Zhang Y, He Z H, Peña R J, Zhang L P. Allelic variation at the Glu-1 and Glu-3 loci and presence of 1B/1R translocation, and their effects on processing quality in cultivars and advanced lines from autumn-sown wheat regions in China. Scientia Agricultura Sinica, 2005, 38(10): 1944-1950. (in Chinese)

[8]雷振生, 刘丽, 王美芳, 阎俊, 杨攀, 张艳, 何中虎. HMW-GS和LMW-GS组成对小麦加工品质的影响. 作物学报, 2009, 35(2): 203-210.

Lei Z S, Liu L, Wang M F, Yan J, Yang P, Zhang Y, He Z H. Effect of HMW and LMW glutenin subunits on processing quality in common wheat. Acta Agronomica Sinica, 2009, 35(2): 203-210. (in Chinese)

[9]刘建军, 何中虎, Peña R J, 赵振东. 1BL/1RS易位对小麦加工品质的影响. 作物学报, 2004, 30(2): 149-153.

Liu J J, He Z H, Peña R J, Zhao Z D. Effect of 1BL/1RS translocation on grain quality and noodle quality in bread wheat. Acta Agronomica Sinica, 2004, 30(2): 149-153. (in Chinese)

[10]唐怀君, 殷贵鸿, 夏先春, 冯建军, 曲延英, 何中虎. 1BL•1RS特异性分子标记的筛选及其对不同来源小麦品种1RS易位染色体的鉴定. 作物学报, 2009, 35(11): 2107-2115.

Tang H J, Yin G H, Xia X C, Feng J J, Qu Y Y, He Z H. Evaluation of molecular markers specific for 1BL•1RS translocation and characterization of 1RS chromosome in wheat varieties from different origins. Acta Agronomica Sinica, 2009, 35(11): 2107-2115. (in Chinese)

[11]陈东升, Kiribuchi-Otobe C, 徐兆华, 陈新民, 周阳, 何中虎, Yoshida H, 张艳, 王德森. Waxy蛋白缺失对小麦淀粉特性和中国鲜面条品质的影响. 中国农业科学, 2005, 38(5): 865-873.

Chen D S, Kiribuchi-Otobe C, Xu Z H, Chen X M, Zhou Y, He Z H, Yoshida H, Zhang Y, Wang D S. Effect of Wx-A1, Wx-B1, Wx-D1 protein on starch properties and Chinese fresh noodle quality. Scientia Agricultura Sinica, 2005, 38(5): 865-873. (in Chinese)

[12]周淼平, 任丽娟, 蔡士宾, 余桂红, 张旭, 马鸿翔. 小麦糯质基因的分子标记辅助选择. 麦类作物学报, 2006, 26(5): 10-15.

Zhou M P, Ren L J, Cai S B, Yu G H, Zhang X, Ma H X. Molecular maker aided selection of Wx genes in wheat. Journal of Triticeae Crops, 2006, 26(5): 10-15. (in Chinese)

[13]陈静, 张晓科, 何中虎, 孙文瑜, 伍玲, 李莉蓉, 余懋群. 面条品质性状相关基因在四川小麦中的分布. 麦类作物学报, 2007, 27(6): 1010-1015.

Chen J, Zhang X K, He Z H, Sun W Y, Wu L, Li L R, Yu M Q. Distribution of genes associated with noodle qualities in Sichuan wheat cultivars. Journal of Triticeae Crops, 2007, 27(6): 1010-1015. (in Chinese)

[14]葛秀秀, 何中虎, 杨金, 张歧军. 我国冬小麦品种多酚氧化酶活性的遗传变异及其与品质性状的相关分析. 作物学报, 2003, 29(4): 481-485.

Ge X X, He Z H, Yang J, Zhang Q J. Polyphenol oxidase activities of Chinese winter wheat cultivars and correlations with quality characteristics. Acta Agronomica Sinica, 2003, 29(4): 481-485. (in Chinese)

[15]肖永贵, 何心尧, 刘建军, 孙道杰, 夏先春, 何中虎. 中国冬小麦品种多酚氧化酶活性基因等位变异检测及其分布规律研究. 中国农业科学, 2008, 41(4): 954-960.

Xiao Y G, He X Y, Liu J J, Sun D J, Xia X C, He Z H. Molecular identification and distribution of the polyphenol oxidase genes in Chinese winter wheat cultivars. Scientia Agricultura Sinica, 2008, 41(4): 954-960. (in Chinese)

[16]芦静, 何中虎, 夏先春, 吴新元, 李冬, 曹俊梅. 新疆小麦品种高分子量谷蛋白亚基及相关品质基因的分子标记检测. 作物学报, 2009, 35(4): 647-661.

Lu J, He Z H, Xia X C, Wu X Y, Li D, Cao J M. Characterization of Xinjiang local and introduced wheat germplasm for high molecular weight glutenin subunits and quality-related genes with molecular markers. Acta Agronomica Sinica, 2009, 35(4): 647-661. (in Chinese)

[17]张立平, 阎俊, 夏先春, 何中虎, Sutherland M W. 普通小麦籽粒黄色素含量的QTL分析. 作物学报, 2006, 32(1): 41-45.

Zhang L P, Yan J, Xia X C, He Z H, Sutherland M W. QTL mapping for kernel yellow pigment content in common wheat. Acta Agronomica Sinica, 2006, 32(1): 41-45. (in Chinese)

[18]杨芳萍, 何心尧, 何中虎, 尚勋武, 杨文雄, 夏先春. 中国小麦品种黄色素含量等位变异分子检测及其分布规律研究. 中国农业科学, 2008, 41(10): 2923-2930.

Yang F P, He X Y, He Z H, Shang X W, Yang W X, Xia X C. Molecular detection and distribution of allelic variations of a gene for yellow pigment content in Chinese winter wheat cultivars. Scientia Agricultura Sinica, 2008, 41(10): 2923-2930. (in Chinese)

[19]胡凤灵, 何中虎, 葛建贵, 姜文武, 时萍, 夏先春. 小麦品种黄色素含量和多酚氧化酶活性基因的分子标记检测. 麦类作物学报, 2011, 31(1): 47-53.

Hu F L, He Z H, Ge J G, Jiang W W, Shi P, Xia X C. Identification of genes for yellow pigment content and polyphenol oxidase activity in common wheat using molecular markers. Journal of Triticeae Crops, 2011, 31(1): 47-53. (in Chinese)

[20]陈锋, 陈东升, 钱森和, 张燕, 夏先春, 何中虎. Puroindoline基因对春小麦磨粉及馒头、面条品质的影响. 作物学报, 2006, 32(7): 980-986.

Chen F, Chen D S, Qian S H, Zhang Y, Xia X C, He Z H. Influence of puroindoline gene on milling performance, steamed bread and noodle qualities in spring wheat. Acta Agronomica Sinica, 2006, 32(7): 980-986. (in Chinese)

[21]陈锋, 何中虎, 陈东升, 张春利, 夏先春. 中国春小麦籽粒硬度puroindoline基因等位变异检测. 中国农业科学, 2007, 40(2): 217-224.

Chen F, He Z H, Chen D S, Zhang C L, Xia X C. Allelic variation of puroindoline genes in Chinese spring wheats. Scientia Agricultura Sinica, 2007, 40(2): 217-224. (in Chinese)

[22]郭世华, 岳淑芳, 王金霞, 侯国锋. 小麦籽粒硬度及其Pinb-D1基因等位变异的STS标记检测. 麦类作物学报, 2008, 28(4): 600-606.

Guo S H, Yue S F, Wang J X, Hou G F. Grain hardness and detection of Pinb-D1 alleles variation in wheat by STS molecular markers. Journal of Triticeae Crops, 2008, 28(4): 600-606. (in Chinese)

[23]杨燕, 赵献林, 张勇, 陈新民, 何中虎, 于卓, 夏兰琴. 四个小麦抗穗发芽分子标记有效性的验证与评价. 作物学报, 2008, 34(1): 17-24.

Yang Y, Zhao X L, Zhang Y, Chen X M, He Z H, Yu Z, Xia L Q. Evaluation and validation of four molecular markers associated with pre-harvest sprouting tolerance in Chinese wheats. Acta Agronomica Sinica, 2008, 34(1): 17-24. (in Chinese)

[24]苗西磊, 王德森, 夏兰芹, 张运宏, 王忠伟, 何中虎, 陈新民. 白粒小麦品种（系）穗发芽抗性机制分析. 麦类作物学报, 2011, 31(4): 741-746.

Miao X L, Wang D S, Xia L Q, Zhang Y H, Wang Z W, He Z H, Chen X M. Analysis on the mechanism of pre-harvest sprouting resistance in white-grain wheat. Journal of Triticeae Crops, 2011, 31(4): 741-746. (in Chinese)

[25]孙永伟, 聂丽娜, 马有志, 徐兆师, 夏兰琴. 小麦抗穗发芽相关Vp1基因启动子的分离及功能验证. 作物学报, 2011, 37(10): 1743-1751.

Sun Y W, Nie L N, Ma Y Z, Xu Z S, Xia L Q. Cloning and functional analysis of Viviparous-1 promoter in wheat. Acta Agronomica Sinica, 2011, 37(10): 1743-1751. (in Chinese)

[26]Ma W, Zhang W, Gale K R. Multiplex-PCR typing of high molecular weight glutenin alleles in wheat. Euphytica, 2003, 134: 51-60.

[27]Lei Z S, Gale K R, He Z H, Gianibelli C, Larroque O, Xia X C, Butow B J, Ma W. Y-type gene specific markers for enhanced discrimination of high-molecular weight glutenin alleles at the Glu-B1 locus in hexaploid wheat. Journal of Cereal Science, 2006, 43: 94-101.

[28]D’Ovidio R, Anderson O D. PCR analysis to distinguish between alleles of a member of multigene family correlated with wheat bread-making quality. Theoretical and Applied Genetics, 1994, 88: 759-763.

[29]Wang L H, Li G Y, Peña R J, Xia X C, He Z H. Development of STS markers and establishment of multiplex PCR for Glu-A3 alleles in common wheat (Triticum aestivum L.). Journal of Cereal Science, 2010, 51: 305-312.

[30]Wang L H, Zhao X L, He Z H, Ma W, Appels R, Peña R J, Xia X C. Characterization of low-molecular-weight glutenin subunit Glu-B3 genes and development of STS markers in common wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 2009, 118: 525-539.

[31]Nakamura T, Vrinten P, Saito M, Konda M. Rapid classification of partial waxy wheats using PCR-based markers. Genome, 2002, 45: 1150-1156.

[32]Sun D J, He Z H, Xia X C, Zhang L P, Morris C F, Apples R, Ma W J, Wang H. A novel STS marker for polyphenol oxidase activity in bread wheat. Molecular Breeding, 2005, 16: 209-218.

[33]He X Y, He Z H, Zhang L P, Sun D J, Morris C F, Fuerst E P, Xia X C. Allelic variation of polyphenol oxidase (PPO) genes located on chromosomes 2A and 2D and development of functional markers for the PPO genes in common wheat. Theoretical and Applied Genetics, 2007, 115: 47-58.

[34]He X Y, Zhang Y L, He Z H, Wu Y P, Xiao Y G, Ma C X, Xia X C. Characterization of phytoene synthase 1 gene (Psy 1) located on common wheat chromosome 7A and development of a functional marker. Theoretical and Applied Genetics, 2008, 116: 213-221.

[35]He X Y, He Z H, Ma W, Appels R, Xia X C. Allelic variants of phytoene synthase 1 (Psy 1) genes in Chinese and CIMMYT wheat cultivars and development of functional markers for flour colour. Molecular Breeding, 2009, 23: 553-563.

[36]Giroux M J, Morris C F. A glycine to serine change in puroindoline b is associated with wheat grain hardness and low levels of starch-surface friabilin. Theoretical and Applied Genetics, 1997, 95: 857-864.

[37]Yang Y, Zhao X L, Xia L Q, Chen X M, Xia X C, Yu Z, He Z H, Röder M. Development and validation of a Viviparous-1 STS marker for pre-harvest sprouting tolerance in Chinese wheats. Theoretical and Applied Genetics, 2007, 115: 971-980.

[38]Francis H A, Leitch A R, Koebner R M D. Conversion of a RAPD-generated PCR product, containing a novel dispersed repetitive element, into a fast and robust assay for the presence of rye chromatin in wheat. Theoretical and Applied Genetics, 1995, 90: 636-642.

[39]刘迎春, 朱慧兰, 程顺和, 马正强. 小麦Wx-A1和Wx-D1位点的PCR分子标记. 麦类作物学报, 2005, 25(1): 1-5.

Liu Y C, Zhu H L, Cheng S H, Ma Z Q. PCR-based molecular markers for Wx-A1 and Wx-D1 genes of wheat. Journal of Triticeae Crops, 2005, 25(1): 1-5. (in Chinese)

[40]Lagudah E S, Appels R, McNeil D. The Nor-D3 locus of Triticum tauschii: Natural variation and genetic linkage to markers in chromosome 5. Genome, 1991, 34(3): 387-395.

[41]何中虎, 晏月明, 庄巧生, 张艳, 夏先春, 张勇, 王德森, 夏兰芹, 胡英考, 蔡民华, 陈新民, 阎俊, 周阳. 中国小麦品种品质评价体系建立与分子改良技术研究. 中国农业科学, 2006, 39(6): 1091-1101.

He Z H, Yan Y M, Zhuang Q S, Zhang Y, Xia X C, Zhang Y, Wang D S, Xia L Q, Hu Y K, Cai M H, Chen X M, Yan J, Zhou Y. Establishment of quality evaluation system and utilization of molecular methods for the improvement of Chinese wheat quality. Scientia Agricultura Sinica, 2006, 39(6): 1091-1101. (in Chinese)

[42]何中虎, 夏先春, 陈新民, 庄巧生. 中国小麦育种进展与展望. 作物学报, 2011, 37(2): 202-215.

He Z H, Xia X C, Chen X M, Zhuang Q S. Progress of wheat breeding in China and the future perspective. Acta Agronomica Sinica, 2011, 37(2): 202-215. (in Chinese)

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