Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (14): 2923-2930.doi: 10.3864/j.issn.0578-1752.2012.14.015

• HORTICULTURE • Previous Articles     Next Articles

Analysis of GhAOS Expression Characteristics in Gladiolus hybridus and Overexpression in Arabidopsis

 LIAN  Qing-Long, XIN  Hai-Bo, LI  Xiao-Xin, ZHONG  Xiong-Hui, YIN  Yi-Lei, YI  Ming-Fang   

  1. 1.农业部规划设计研究院设施农业研究所，北京 100125
    2.中国农业大学观赏园艺与园林系，北京 100193
    3.中国科学院植物分子生理学重点实验室，北京 100093
  • Received:2012-03-02 Online:2012-07-15 Published:2012-04-28

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Abstract: 【Objective】The objective of this study is to analyze the expression pattern of GhAOS and the effect of this gene on JAs biosynthesis in Gladiolus hybridus, and study the response mechanism of the GhAOS gene to damage stress in plant.【Method】The technology of gene gun bombardment was used to analyze the sub-cellular localization of GhAOS. The real time RT-PCR was used to analyze the expression pattern of GhAOS. Agrobacterium-mediated genetic transformation system of Arabidopsis was also used to analyze the overexpression of GhAOS. 【Result】The result of sub-cellular localization showed that the GhAOS-GFP fussion protein was targeted into the chloroplast thylakoid membrance. Real time RT- PCR analysis showed that the relatively high expression level of GhAOS was observed in stolons and cormels, and the expression level in corms steadily increased under MJ treatment with a raising concentration gradients from 0.1 mmol•L-1 to 0.5 mmol•L-1. Meanwhile, the endogenous MJ content was also correlated with the expression level of GhAOS. In addition, the salt tolerance and drought-resistance were increased in the overexpression Arabidopsis plants, and the related resistance genes and the endogenous MJ content were increased after mechanical damage.【Conclusion】GhAOS gene promoted JAs biosynthesis in Gladiolus hybridus, and increased the salt tolerance and drought-resistance in Arabidopsis. AOS gene expression may play a major readjustment function on the signal of JA in Gladiolus hybridus, and it may be closely related with the activation of some defense genes. After mechanical damage , the expression level of related resistance genes and the content of endogenous MJ were increased.

Key words: Gladiolus hybridus, GhAOS, MJ, expression, overexpression

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