褪黑激素对Bcl-2和Bax基因在绒山羊皮肤中表达的影响

doi:10.3864/j.issn.0578-1752.2012.12.015

Abstract

Abstract: 【Objective】The present study was aimed to research the effects of melatonin on the expression of Bcl-2 and Bax genes in the skin of Inner Mongolia Cashmere goat, then discuss the relationships between Bcl-2, Bax, and melatonin and the growth and apoptosis of cashmere goat. 【Method】 Eight Inner Mongolia Cashmere goats, with the same age, sex and liveweight were chosen. Four of them were treated with melatonin, the rest were used as control. Total RNA of skin was extracted, and expression of Bcl-2 and Bax genes were analyzed by real-time PCR. 【Result】 The changing characteristics of Bcl-2/Bax coincided with the growth rhythm of cashmere of Inner Mongolia Cashmere goat. Melatonin had no significant effects on expression of Bcl-2 gene (P>0.05), but significantly up-regulated the Bax gene (P<0.01). 【Conclusion】 Melatonin down-regulated the ratio of Bcl-2/Bax in the skin of Inner Mongolia Cashmere goat, which promoted the apoptosis and accelerated the metabolism.

Key words: cashmere goat, melatonin, Bcl-2 gene, Bax gene

LIU Hui, ZHANG Yan-Jun, LI Jin-Quan, ZHANG Wen-Guang, WANG Zhi-Xin, ZHANG Yong-Bin, HE Yun-Mei, JIANG Zai-Ping, LI Chun, FENG Lin, WANG Rui-Jun. Effects of Melatonin on the Expression of Bcl-2 and Bax Genes in Skin of Cashmere Goat[J].Scientia Agricultura Sinica, 2012, 45(12): 2462-2467.

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References

[1]刘海英, 杨桂芹, 张微, 朱晓萍, 贾志海. FGF5基因对内蒙古绒山羊绒毛性状的影响. 遗传, 2009, 31(2): 175-179.

Liu H Y, Yang G Q, Zhang W, Zhu X P, Jia Z H. Effects of FGF5 gene on the cashmere treat of Inner Mongolia cashmere goat. Hereditas, 2009, 31(2): 175-179. (in Chinese)

[2]Houssay A B, Pazo J H, Epper C E. Effects of the pineal gland upon the hair cycles in mice. The Journal of Investigative Dermatology, 1966, 47(3): 230-234.

[3]Slominski A, Tobin D J, Shibahara S, Wortsman J. Melanin pigmentation in mammalian skin and its hormonal regulation. Physiological Reviews, 2004, 84(4): 1155-1228.

[4]Kobayashi H, Kromminga A, Dunlop T W, Tychsen B , Conrad F, Suzuki N, Memezawa A, Bettermann A, Aiba S, Carlberg C, Paus R. A role of melatonin in neuroectodermal-mesodermal interactions: the hair follicle synthesizes melatonin and expresses functional melatonin receptors. The FASEB Journal Express Article, 2005, 19(12): 1710-1712.

[5]Fischer T W, Slominski A, Tobin D J, Paus R. Melatonin and the hair follicle. Journal of Pineal Research, 2008, 44(1): 1-15.

[6]Muller-Rover S, Rossiter H, Lindner G, Peters E M J, Kupper T S, Paus R. Hair follicle apoptosis and Bcl-2. Journal of Investigative Dermatology Symposium Proceedings, 1999, 4: 272-277.

[7]常子丽. 持续埋植褪黑素对绒山羊绒毛生长特性和相关基因影响的研究[D]. 呼和浩特: 内蒙古农业大学, 2010.

Chang Z L. Study on effect of constant-release melatonin implants on the cashmere growth traits of cashmere goat and relative gene[D]. Hohhot: Inner Mongolia Agriculture University, 2010. (in Chinese)

[8]Onur R, Semercioz A, Orhan I, Yekeler H. The effects of melatonin and the antioxidant defence system on apoptosis regulator proteins (Bax and Bcl-2) in experimentally induced varicocele. Urological Research, 2004, 32: 204-208.

[9]Sedlak T W, Oltvai Z N, Yang E, Wang K, Boise L H, Thompson C B, Korsmeyer S J. Multiple Bcl-2 family members demonstrate selective dimerizations with Bax. Proceedings of the National Academy of Sciences of the United States of America, 1995, 92(17): 7834-7838.

[10]Krajewski S, Blomqvist C, Franssila K, Krajewska M V, Wasenius M, Niskanen E, Nordling S, Reed J C. Reduced expression of proapoptotic gene BAX is associated with poor response rates to combination chemotherapy and shorter survival in women with metastatic breast adenocarcinoma. Cancer Research, 1995, 55(19): 4471-4478.

[11]Muller-Rover S, Rossiter H, Paus R, Handjiski B, Peters E M J, Murphy J E, Mecklenburg L, Kupper T S. Overexpression of Bcl-2 protects from ultraviolet B-induced apoptosis but promotes hair follicle regression and chemotherapy-induced alopecia. The American Journal of Pathology, 2000, 156(4): 1395-1405.

[12]Yoo Y M, Jung E M, Choi K C, Jeung E B. Effect of melatonin on mRNA expressions of transcription factors in murine embryonic stem cells. Brain Research, 2011, 1385: 1-7.

[13]狄荣科, 徐金, 周志强, 刘益仁. 褪黑激素影响帕金森病模型大鼠前脑纹状体细胞的凋亡. 中国临床康复, 2006, 10(42): 95-98.

Di R K, Xu J, Zhou Z Q, Li Y R. Role of melatonin in the forebrain striatum apoptosis in rat models of Parkinson disease. Chinese Journal of Clinical Rehabilitation, 2006, 10(42): 95-98. (in Chinese)

[14]Oltval Z N, Milliman C L, Korsmeyer S J. Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programed cell death. Cell, 1993,74(4): 609-619.

[15]Paradis E, Douillard H, Koutroumanis M, Goodyer C, LeBlanc A. Amyloid beta peptide of Alzheimer’s disease down regulates Bcl-2 and upregulates Bax expression in human neurons. Journal of Neurosci, 1996, 16: 7533-7539.

[16]陈伟, 付小兵, 孙同柱, 孙晓庆, 赵志力, 盛志勇. Bax和Bcl-2在增生性瘢痕中的表达特征及其意义. 创伤外科杂志, 2002, 4(5): 276-278.

Chen W, Fu X B, Sun T Z, Sun X Q, Zhao Z L, Sheng Z Y. Characteristic of Bcl-2 and Bax expression in hypertrophic scars and its biological significance. Department of Traumatology, 2002, 4(5): 276-278. (in Chinese)

[17]岳春旺, 张微, 孔祥浩, 刘海英, 贾志海. 褪黑激素对内蒙古白绒山羊产绒性能的影响. 中国畜牧杂志, 2007, 43(7): 32-34.

Yue C W, Zhang W, Kong X H, Liu H Y, Jia Z H. Effects of melatonin on the cashmere treats of Inner Mongolia cashmere goats. China Livestock, 2007, 43(7): 32-34. (in Chinese)

[18]李金泉, 尹俊, 周欢敏. 绒山羊绒毛生长调控机理研究进展. 中国草食动物, 2001, 3(3): 41-44.

Li J Q, Yin J, Zhou H M. Reserch progress of regulate mechanism of cashmere groth of cashmere goat. China Herbivore, 2001, 3(3): 41-44. (in Chinese)

[19]Nixon A J, Choy V J, Parry A L, Pearson A J. Fiber growth initiation in hair follicles of goats treated with melatonin. Journal of Experimental Zoology, 1993, 267(1): 47-56.

[20]Allain D, Ravault J, Panaretto B, Rougeot J. Effects of pinealectomy on photoperiodic control of hair follicle activity in the Limousine ram: possible relationships with plasma prolactin levels. Journal of Pineal Research, 1986, 3(1): 25-32.

[21]Ibraheem M, Galbraith H, Scaife J, Ewen S. Growth of secondary hair follicles of the cashmere goat in vitro and their response to prolactin and melatonin. Journal of Anatomy, 1994, 185(1): 135-142.

[22]Webster J, Suttie J, Corson I. Effects of melatonin implants on reproductive seasonality of male red deer (Cervus elaphus). Reproduction, 1991, 92(1): 1-11.

[23]柳建昌, 桂荣, 赵青山. 褪黑素对内蒙阿白山羊在非生绒季节促进绒生长及绒产量的影响. 动物学杂志, 1994, 29(3): 46-50.

Liu J C, Gui R, Zhao Q S. Effects of melatonin on promoting the cashmere growth and yield in the non-cashmere growth season of Inner mongolia ALPAS cashmere goats. Zoology, 1994, 29(3): 46-50. (in Chinese)

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Effects of Melatonin on the Expression of Bcl-2 and Bax Genes in Skin of Cashmere Goat

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