外种猪SLA-MS分型与免疫参数的关联

doi:10.3864/j.issn.0578-1752.2012.05.020

Abstract

Abstract: 【Objective】 The polymorphisms and the haplotypes of the swine leukocyte antigen complex (SLA) were investigated in Landrace and Yorkshire pigs using microsatellites (MS). The association between haplotypes and immune parameters was analyzed in order to provide references for disease resistance breeding in pigs.【Method】The genetic diversity parameters were calculated by thirteen microsatellites, which were in SLA region, the haplotypes in class Ⅰ and class Ⅱ were typed by some selected microsatellites typing, and the association between haplotypes and immune parameters were studied by microsatellites in class Ⅰand class Ⅱ regions.【Result】The expected heterozygosity ( =0.70) and polymorphism information content ( =0.65) of Yorkshire pigs were higher than those（ =0.67， =0.62）of Landrace pigs, respectively. The multiple comparison results of six haplotypes and immune parameters in SLA Ⅰ showed that the mean corpuscular hemoglobin concentration (MCHC) of H2 was higher than that of H3 (P＜0.05) and H5 (P＜0.01), respectively. The platelet distribution width (PDW) of H2 was lower than that of H3 (P＜0.05). The results of multiple comparisons between twelve haplotypes and immune parameters in SLAⅡ showed that the mean of red blood cell count (RBC) of H1′ was lower than that of H6′ and H9′ (P＜0.05), respectively. The value of lymphocytes ratio (LYM) of H1′ was lower than that of H7′ (P＜0.05), and extremely lower than that of H6′, H9′ and H11′ (P＜0.01), respectively. The mean of MCHC of H6′was lower than that of H7′ (P＜0.05), H1′ and H11′ (P＜0.01), while the value of MCHC of H9′ was lower than that of H1′, H7′ and H11′ (P＜0.05), respectively. The mean corpuscular volume (MCV) of H9′ was lower than that of H1′, H6′ and H11′ (P＜0.05) and extremely lower than that of H7′ (P＜0.01), respectively. The value of monocytes ratio (MONO) of H11′ was significantly lower than that of H6′, H7′ (P＜0.05) and H1′ (P＜0.01), while the value of H6′, H7′and H9′ was lower than that of H1′ (P＜0.05, P＜0.01), respectively.【Conclusion】The polymorphisms of Yorkshire pigs were higher than those of Landrace pigs, and the associations were detected between some haplotypes of SLA-MS and immune parameters, which were PDW, MCHC, RBC, MCV, LYM and MONO. These results could be used in evaluation of health index in future.

Key words: SLA, microsatellite typing, haplotypes, principal component analysis, immune parameters

YU Hui, LIU Rong-Hui, ZUO Qi-Zhen, LI Yan, LI Hua. The Association Between the SLA Microsatellites Typing and the Immune Parameters of Introduced Pig Breeds[J].Scientia Agricultura Sinica, 2012, 45(5): 981-989.

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The Association Between the SLA Microsatellites Typing and the Immune Parameters of Introduced Pig Breeds

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