开放式臭氧浓度升高条件下转Bt（Cry1Ab/Ac）基因水稻的光合特性

doi:10.3864/j.issn.0578-1752.2012.03.003

Abstract

Abstract: 【Objective】 The purpose of this study was to identify the effects on photosynthetic characteristics of transgenic Bt rice (Oryza sativa L.) under elevated O3 concentration during the period of May-October of 2010. 【Method】 Pot experiments were conducted with Bt-Shanyou 63 (hereinafter called Bt-SY63, a transgenic rice with Cry1Ab/Ac) and Shanyou 63 (hereinafter called SY63, a hybrid indica cultivar) at 26 d, 47 d and 75 d of ozone treatment, respectively, the indicators relating to the photosynthetic characteristic were measured under Ozone Free Air Controlled Enrichment Conditions (O3-FACE). 【Result】 The results showed that the net photosynthetic rate of flag leaves in Bt-SY63 and SY63, compared with the control, took on a significantly decline up to 21.1% and 15.1% on 75 d (P＜0.01), respectively, by prolonged elevated O3 exposure. The downtrend of stomatal conductance and transpiration was also consistent with Pn. As for the chlorophyll fluorescence kinetics parameters, the maximal photochemical efficiency of PSⅡ, actual photochemical efficiency of PSⅡ and photochemical quenching all descended by elevated O3 concentration while non-photochemical quenching and fraction of light absorbed in PSⅡ which was dissipated in the PSⅡ antenna in varied ranges companied with Pn enhanced. The content of chlorophyll and carotenoid of the two varieties also declined, the same with the total soluble protein content in SY63. However, the alteration of TSP content in Bt-SY63 was in perfectly opposite to the SY63’s, there was no remarkable decrease in ribulose-1,5-bisphosphate carboxylas/oxygenase contents in Bt-SY63. 【Conclusion】 Photosynthetic parameters had presented to be changed in leaves of the genetic modified rice and the traditional plant under the O3-FACE conditions, with a larger amplitude for Bt-SY63 compared to SY63. In conclusion, the results suggested that Bt-SY63 was more sensitive to elevated O3 concentration than SY63 coupled with great volatility.

Key words: elevated ozone, transgenic Bt rice, photosynthetic characteristic

LI Chun-Hua, ZHU Jian-Guo, LIU Biao, ZENG Qing, LUO Ke-Ju, TANG Hao-Ye, LIU Gang, ZHANG Guo-An. Photosynthetic Characteristics of Transgenic Bt (Cry1Ab/Ac) Rice Under Ozone Free Air Controlled Enrichment Conditions[J].Scientia Agricultura Sinica, 2012, 45(3): 426-434.

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Photosynthetic Characteristics of Transgenic Bt (Cry1Ab/Ac) Rice Under Ozone Free Air Controlled Enrichment Conditions

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