生防菌哈茨木霉T4对黄瓜根围土壤细菌群落的影响

doi:10.3864/j.issn.0578-1752.2012.02.006

Abstract

Abstract: 【Objective】The objective of this study is to analysis the impact of T. harzianum T4 introduction on the indigenous bacterial community of rhizosphere soil, and to provide objective biosafety evaluations for the field application of biocontrol agents. 【Method】 Comprised molecular tools DGGE and T-RFLP were used in this study to assess the modifications of the bacterial community in cucumber rhizosphere after inoculation with T. harzianum T4. 【Result】 Introduction of T. harzianum T4 in the soil resulted in significant variations of the bacterial community structures from 14 to 56 days post-inoculation. During this period the impact of T4 was sustainable and weakened gradually. T. harzianum T4 could restrain effectively Cyanobacterium, Beta-proteobacterium, Staphylococcus, Burkholderia, Serratia, Acidobacterium and some kinds of soil uncultured bacterium, while it improved the growth of Bacillus, Agrobacterium and Gemmatimonadetes observably. On 70th day after T. harzianum T4 introduced, as 2 weeks after harvest, there was no prominent alteration of bacterial community due to inoculation with T4. 【Conclusion】 Tracking of the modifications of the indigenous bacterial community by 16S rDNA DGGE and T-RFLP analysis not only described comprehensively the variations of the structure of soil bacterial community, but also showed the preliminary and qualitative information of bacterial community change. In general, the application of T. harzianum T4 exerted only transient impact on the bacterial community of cucumber rhizosphere in systematic field investigation, but the perturbation was unable to imperil the bacterial community stability in the long-term.

Key words: Trichoderma harzianum T4, soil bacterial community, T-RFLP, DGGE

YIN Dan-Han, GAO Guan-Peng, XIA Fei, WANG Wei. Effects of the Introduction of Biocontrol Agent Trichoderma harzianum T4 on the Bacterial Community in Cucumber Rhizosphere[J].Scientia Agricultura Sinica, 2012, 45(2): 246-`254.

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Effects of the Introduction of Biocontrol Agent Trichoderma harzianum T4 on the Bacterial Community in Cucumber Rhizosphere

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