Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (22): 4636-4644.doi: 10.3864/j.issn.0578-1752.2011.22.010

• HORTICULTURE • Previous Articles     Next Articles

Effects of Watermelon Cultivars with Different Resistances to Fusarium oxysporum f. sp. niveum and Grafting on Rhizosphere  Soil Microorganism Population and Community Structure

 WU  Feng-Zhi, AN  Mei-Jun   

  1. 1.东北农业大学园艺学院，哈尔滨 150030
    2.黑龙江省蚕蜂技术指导总站，哈尔滨150090
  • Received:2011-05-27 Online:2011-11-15 Published:2011-09-23

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Abstract: 【Objective】 The purpose of this study is to reveal the soil microbiology mechanism of grafting on increase of plant disease resistance, and the differences of rhizosphere soil microorganism population and community structure of grafted and ungrafted watermelon cultivars with different resistances to Fusarium oxysporum f.sp. niveum were evaluated. 【Method】 Changes of rhizosphere soil microorganism population and community of own-rooted watermelon cultivars (Tianniu, resistant cultivar; and Tianshi, susceptible cultivar), rootstock (Boqiang No.1, pumpkin) and their grafted plants were investigated by plate culture method and PCR-DGGE method at different watermelon growth stages. 【Result】 Plate culture results showed that the rhizosphere culturable bacteria population of resistant watermelon cultivar was significantly larger than that of the susceptible cultivar, while the fungi population was significantly smaller than that of the susceptible cultivar of the watermelon at middle-late growing stage. The rhizosphere culturable bacteria and actinomyces populations of the grafted susceptible cultivar were larger than the ungrafted one, but the Fusarium population in rhizosphere soil of grafted watermelon was smaller than that of the ungrafted. PCR-DGGE results showed that rhizosphere soil bacteria and fungi community structures of all treatments changed with watermelon growth stages, and were different between different treatments. Further sequencing analysis of specific bands from DGGE profiles indicated that different groups of bacteria and fungi occurred in the rhizosphere of different watermelon cultivars and rootstock Pupkin cultivar. 【Conclusion】 The resistant watermelon cultivar had significant higher quantities of rhizosphere soil bacteria and actinomyces compared with the susceptible one, while the resistant watermelon cultivar had significant lower quantities of rhizosphere soil fungi and Fusarium compared with the susceptible one. The resistance of watermelon cultivar was closely related to the size of rhizosphere microorganism population. The grafting treatment significantly improved rhizosphere soil bacteria and actinomyces quantities and reduced the quantities of rhizosphere soil Fusarium of susceptible cultivar. The grafting treatment also changed soil microbial community structures. It was concluded that plant genotype has a significant impact on soil microbial community structure, and the differences in the rhizosphere microbial community may contribute to the differences in resistance to F. oxysporum f. sp. Niveum and cultivars.

Key words: watermelon, Fusarium oxysporum f. sp. niveum, grafting, microbial population and community structure, PCR-DGGE

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