Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (24): 5100-5107.doi: 10.3864/j.issn.0578-1752.2011.24.016

• VETERINARY SCIENCE • Previous Articles     Next Articles

Characteristics of Pathogenic and HA Antigenic Variation of H9N2 Subtybe Avian Influenza Viruses Isolated from 1998 to 2008 in China

 CHEN  Lu, LIU  Shou-Chuan, ZHAO  Jun, WANG  Chuan-Qing, WANG  Ze-Lin   

  1. 1.河南农业大学禽病研究所，郑州 450002
  • Received:2010-04-15 Online:2011-12-15 Published:2011-10-18

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Abstract: 【Objective】 The objective of this experiment is to investigate the characteristics of pathogenic and antigenic variation of H9N2 subtybe avian influenza viruses isolated from 1998 to 2008 in China. 【Method】 The EID50, ELD50, MDT, ICPI, IVPI and the duration of shedding virus from infected 8-week-old SPF chickens of different H9N2 avian influenza viruses isolates were determined. The HI and VN activity of monoclonal antibody 2A4 and F6 on different H9N2 avian influenza viruses isolates were assayed and the HA genes of different antigenic reactive isolates were sequenced and analyzed. 【Result】 The determined pathogenicity suggested that the virulence of different isolates were different, thereinto, 3#, 12#, and 14# isolates showed higher pathogenicity than the others and could cause the death of SPF chickens. The 8-week-old SPF chickens infected by 3# or 12# isolate shed virus earlier and last for a longer time. 3 # and 12 # isolates showed specific response properties to monoclonal antibody 2A4 and F6. Monoclonal antibody 2A4 and F6 could inhibit the hemagglutinin activity of 3 # and 12 # isolates, however it could not neutralize the virus infection on CEF cells. HA sequence analysis showed that there was a single amino acid substitution of Ser (S)-to- Asn(N) at position 145 in the HA protein of 3 # and 12 # isolates, which led to the loss of reactivity to the monoclonal antibody 2A4 and F6 and the occurrence of a new potential glycosylation site NGT. The change of reactivity to the monoclonal antibody 2A4 and F6 suggests that the site (S145) is one of HA protein epitope of the H9N2 subtype avian influenza A virus. The new occurring potential glycoprotein site NGT in the HA protein of 3 # and 12 # isolates may cause the enhancing pathogenicity. 【Conclusion】 The results show that H9N2 subtype avian influenza virus isolates have the tendency to evolve, resulting to the occurrence of mutants which have higher virulence and variable antigenicity. The higher virulent mutants may cause death of chickens and lead to more economic loss. The antigenic mutants may evade the immunity, which pose a new challenge to the immune prevention for the H9N2 avian influenza.

Key words: avian influenza virus, H9N2 subtype, HA glycoprotein, pathogenicity, antigenic variation

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