Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (23): 4952-4961.doi: 10.3864/j.issn.0578-1752.2011.23.024

• RESEARCH NOTES • Previous Articles     Next Articles

Effect of Tomato GMPase Overexpression on Tolerance of Potato Plants to Temperature Stress

 LI  Chao-Han, LI  Qing-Zhu, SHI  Qing-Hua, BAI  Long-Qiang, GUO  Xiao-Qing, LI  Xia, YU  Xian-Chang   

  1. 1.中国农业科学院蔬菜花卉研究所，北京100081
    2.山东农业大学园艺科学与工程学院/作物生物学国家重点实验室，山东泰安271018
  • Received:2011-05-06 Online:2011-12-01 Published:2011-09-20

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Abstract: 【Objective】 The aim of the study was to clarify the function of the GDP-D-mannose pyrophosphorylase (GMPase) in the L-galactose pathway of ascorbic acid (AsA) synthesis in plants, and to make sure the relation of GMPase overexpression with ascorbic acid content and temperature-stress tolerance ability. 【Method】 Two transgenic potato strains of overexpressing tomato GMPase were obtained by agrobacterium-mediated transformation. The transgenic plants and wild-type plants were treated in temperature-stress conditions (10/5℃ as low-temperature stress, 35/30℃ as high-temperature stress) for 3 days then in the recover-condition (25/20℃) for 3 days. Real time RT-PCR was used to analyze the expression of GMPase gene. And ascorbic acid content and relative index to temperature stress were also determined. 【Result】Compared with wild-type plants, with the overexpression of GMPase gene in transgenic plants, the GMPase activity and contents of AsA and dehydroascorbate (DHA) in normal and temperature-stress conditions were increased as well as the activities of dehydroascorbate reductase (DHAR), monodehydroascorbate reductase (MDHAR) and ascorbate peroxidase (APX). Meanwhile, the malondialdehyde (MDA) and H2O2 contents and permeability were decreased in the transgenic plants. 【Conclusion】 The overexpression of GMPase may increase potato tolerance to temperature stress by increasing AsA level and lowering reactive oxygen species (ROS).

Key words: potato, ascorbic acid, GDP-D-mannose pyrophosphorylase, transgenic, temperature stress

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