Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (19): 4072-4080.doi: 10.3864/j.issn.0578-1752.2011.19.018

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Polymorphisms of SLC11A1 Gene and Their Association with Mastitis in Chinese Holstein

 GUO  Yang, WANG  Hong-Mei, HOU  Ming-Hai, WANG  Chang-Fa, HE  Hong-Bin, LU Wen-Fa , ZHONG  Ji-Feng   

  1. 1.山东省农业科学院奶牛研究中心
    2.吉林农业大学
  • Received:2010-05-31 Online:2011-10-01 Published:2010-07-14

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Abstract: 【Objective】The polymorphisms of SLC11A1 gene and their correlation to mastitis in Chinese Holstein were investigated for the purpose of providing molecular maker information to facilitate the breeding efficiency of mastitis  resistance. 【Method】CRS-PCR, descend PCR and sequencing were applied to analyze the polymorphisms of four locus in SLC11A1 gene intron 9, exon10 and intron 11 in 771 Chinese Holstein Cattle. The PHASE and SHEsis softwares were used to analyze matching chain disequilibrium and haplotype analysis, respectively. 【Result】 Four SNPs not linking were found, in which 6 067(A/G), 6 358(C/T) and 7 809(A/T) were reported for the first time, and were in intron 9, exon 10 and intron 11, respectively. At locus 6 358 (C/T) and 7 155 (A/G), the cows with genotype CC and AA showed higher milk yield and lower SCS (P＜0.05). Sixteen haplotype combinations were found in 771 Chinese Holstein Cattle, the cows with haplotype combinations CAAA had the higher milk yield and lowest SCS.【Conclusion】Considering the distribution frequencies of haplotype combinations, SLC11A1 gene CAAA haplotype combination is favorable haplotype combinations to the SCS and milk yield and can be chosen as the molecular markers of choosing mastitis resistance selection in dairy cattle.

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