Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (19): 4081-4087.doi: 10.3864/j.issn.0578-1752.2011.19.019

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Phylogenetic Relationships Among Domestic Chinese Bovinae Species Based on Mitochondrial Cytochrome b Gene Sequences

 GENG  Rong-Qing, WANG  Lan-Ping, JI  De-Jun, CHANG  Hong, LI  Yong-Hong, CHANG  Chun-Fang   

  1. 1.盐城师范学院生命科学与技术学院
    2.扬州大学动物科学与技术学院
  • Received:2010-08-20 Online:2011-10-01 Published:2010-12-30

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Abstract: 【Objective】This study was designed to analyze the phylogenetic relationships among domestic Chinese Bovinae species and provide molecular evidence for phylogenetic evolvement.【Method】The complete sequences of mitochondrial cytochrome b gene from six bovine species, including Bos taurus, Bos indicus, Bos grunniens, Bos frontalis, Bubalus bubalis (swamp buffalo) and Bos bubalis (river buffalo), were obtained by PCR and standard double-strand DNA sequencing method. The phylogenetic relationships among different bovine species were analyzed through molecular evolution software.【Result】The complete sequences of mitochondrial cytochrome b gene from six bovine species were all 1 140 bp in length. No insert and deletion mutation was observed. Total 220 variation sites were tested including 213 parsimony informative sites. The number of transition was higher than transversion and the ratio was 5.2, suggesting that the mutation was not saturation. Genetic distances showed that Bos taurus and Bos indicus possessed the most close relationship and the relationships between Bos grunniens, Bos frontalis, swamp water buffalo, river water buffalo and them deduced in turn gradually. Six bovine species distributed four main monophyletic branches and could be divided into Bos, Poephagus, Bibos, Bubalus, respectively. Divergency times among bovine species were between 0.775-6.43 MYA. The longest divergency time was between Asian water buffalo and other bovine species. The shortest divergency time was between Bos taurus and Bos indicus. The monophyletic relationship was not emerged between Bos frontalis and Bos gaurus, Bos frontalis and Bos gaurus did not differentiated clearly on mitochondrial DNA.【Conclusion】Six domestic Chinese Bovinae species could be divided into four branches corresponding to Bos, Poephagus, Bibos and Bubalus, respectively. The relationships between Bos and Poephagus, Bibos, Bubalus deduced in turn gradually. Divergency times among six bovine species were between 0.775-6.43 MYA. There was very close relationship between Bos frontalis and Bos gaurus, which showed that they might share the same maternal origin in an anterior time. The results did not support the opinion that Bos frontalis might be the domesticated form of Bos gaurus, Bos frontalis and Bos gaurus might be the progeny of a kind of extinct wild bovine species.

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[1]Magee D A, Meghen C, Harrison S, Troy C S, Cymbron T, Gaillard C, Morrow A, Maillard J C, Bradley D G. A partial African ancestry for the creole cattle populations of the Caribbean. The Journal of Heredity, 2002, 93(6): 429-432.

[2]Kikkawa Y, Takada T, Nomura S K, Namikawa T, Yonekawa H, Amano T. Phylogenies using mtDNA and SRY provide evidence for male-mediated introgression in Asian domestic cattle. Animal Genetics, 2003, 34: 96-101.

[3]Lirón J P, Bravi C M, Mirol P M, Peral-García P, Giovambattista G. African matrilineages in American Creole Cattle: evidence of two independent continental sources. Animal Genetics, 2006, 37: 379-382.

[4]Ibeagha-Awemu E M, Erhardt G. Genetic structure and differentiation of 12 African Bos indicus and Bos taurus cattle breeds, inferred from protein and microsatellite polymorphisms. Journal of Animal Breeding and Genetics, 2005, 122: 12-20.

[5]Lai S J, Liu Y P, Liu Y X, Li X W, Yao Y G. Genetic diversity and origin of Chinese Cattle revealed by mtDNA D-loop sequence variation. Molecular Phylogenetics and Evolution, 2006, 38: 146-154.

[6]Guo S C, Savolainen P, Su J P, Zhang Q, Qi D L, Zhou J, Zhong Y, Zhao X Q, Liu J Q. Origin of mitochondrial DNA diversity of domestic yaks. BMC Evolutionary Biology, 2006, 6: 73-82.

[7]Lai S J, Chen S Y, Liu Y P, Yao Y G. Mitochondrial DNA sequence diversity and origin of Chinese domestic yak. Animal Genetics, 2007, 38: 77-80.

[8]Gou X, Wang Y, Yang S, Deng W, Mao H. Genetic diversity and origin of gayal and cattle in Yunnan revealed by mtDNA control region and SRY gene sequence variation. Journal of Animal Breeding and Genetics, 2010, 127: 154-160.

[9]Li S P, Chang H, Ma G L, Cheng H Y. Molecular phylogeny of the gayal in Yunnan China inferred from the analysis of cytochrome b gene entire sequences. Asian-Australasian Journal of Animal Sciences, 2008, 21(6): 789-793.

[10]Kierstein G, Vallinoto M, Silva A, Schneider M P, Iannuzzi L, Brenig B. Analysis of mitochondrial D-loop region casts new light on domestic water buffalo (Bubalus bubalis) phylogeny. Molecular Phylogenetics and Evolution, 2004, 30: 308-324.

[11]Kumar S, Nagarajan M, Sandhu J S, Kumar N, Behl V. Phylogeography and domestication of Indian river buffalo. BMC Evolutionary Biology, 2007, 7: 186-183.

[12]Lei C Z, Zhang W, Chen H, Lu F, Liu R Y, Yang X Y, Zhang H C, Liu Z G, Yao L B, Lu Z F, Zhao Z L. Independent maternal origin of Chinese Swamp Buffalo (Bubalus bubalis). Animal Genetics, 2007, 38: 97-102.

[13]Janecek L L, Honeycutt R L, Adkins R M, Davis S K. Mitochondrial gene sequences and the molecular systematics of the artiodactyl subfamily bovinae. Molecular Phylogenetics and Evolution, 1996, 6(1): 107-119.

[14]Hassanin A, Douzery E J P. The tribal radiation of the family Bovidae (Artiodactyla) and the evolution of the mitochondrial cytochrome b gene. Molecular Phylogenetics and Evolution, 1999, 13(2): 227-243.

[15]Birungi J, Arctander P. Molecular systematics and phylogeny of the reduncini (Artiodactyla: Bovidae) inferred from the analysis of mitochondrial cytochrome b gene sequences. Journal of Mammalian Evolution, 2001, 8(2): 125-147.

[16]Hassanin A, Ropiquet A. Molecular phylogeny of the tribe Bovini (Bovidae, Bovinae) and the taxonomic status of the kouprey, Bos sauveli Urbain 1937. Molecular Phylogenetics and Evolution, 2004, 33: 896-907.

[17]樊宝良, 李  宁, 吴常信. 依据乳蛋白基因序列构建反刍动物种系发生树的研究. 遗传学报, 2000, 27(6): 485-497.

Fan B L, Li N, Wu C X. Research on constructing phylogenetics trees of ruminants basing on the database of milk protein gene sequences. Acta Genetica Sinica, 2000, 27(6): 485-497. (in Chinese)

[18]郭松长, 刘建全, 祁得林, 杨  洁, 赵新全. 牦牛的分类学地位及起源研究：mtDNA D-loop序列的分析. 兽类学报, 2006, 26(4): 325-330.

Guo S C, Liu J Q, Qi D L, Yang J, Zhao X Q. Taxonomic placement and origin of yaks: implications from analyses of mtDNA D-loop fragment sequences. Acta Theriologica Sinica, 2006, 26(4): 325-330. (in Chinese)

[19]李齐发, 李隐侠, 赵兴波, 刘振山, 张庆波, 宋大伟, 屈旭光, 李  宁, 谢  庄. 牦牛线粒体DNA 细胞色素b基因序列测定及其起源、分类地位研究. 畜牧兽医学报, 2006, 37(11): 1118-1123.

Li Q F, Li Y X, Zhao X B, Liu Z S, Zhang Q B, Song D W, Qu X G, Li N, Xie Z. Sequencing cytochrome b gene of mitochondrial DNA in yak and researching its origin and taxonomic status. Acta Veterinaria et Zootechnica Sinica, 2006, 37(11): 1118-1123. (in Chinese)

[20]Thompson J D, Higgins D G, Gibson T J. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 1994, 22(22): 4673-4680.

[21]Tamura K, Dudley J, Nei M, Kumar S. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Biology and Evolution, 2007, 24(8):1596-1599.

[22]Knight A, Mindell D P. Substitution bias, weighting of DNA sequence evolution, and the phylogenetic positions of fea’s viper. Systematic Biology, 1993, 42(1): 18-31.

[23]常  洪. 动物遗传资源学. 北京: 科学出版社, 2009.

Chang H. Animal Genetic Resources. Beijing: Science Press, 2009. (in Chinese)

[24]Ritz L R, Glowatzki-Mullis M L, MacHugh D E, Gaillard C. Phylogenetic analysis of the tribe Bovini using microsatellites. Animal Genetics, 2000, 31: 178-185.

[25]Zhang Y P, Ryder O A. Different Rates of mitochondrial DNA sequence evolution in Kirk’s Dik-dik (Madoqua-kirkii) populations. Molecular Phylogenetics and Evolution, 1995, 4(3): 291-297.

[26]Walker E P, Warmick F, Hamlet S T. Mammals of the World. Balttmore: the Johns Hopkins Press, 1968.

[27]Winter H, Mayr B, Schleger W, Dworak E, Krutzler J, Burger B. Karyotyping, red blood cell and haemoglobin typing of the mithun (Bos frontalis), its wild ancestor and its hybrids. Research in Veterinary Science, 1984, 36(3): 276-283.

[28]Tanaka K, Mannen H, Kurosawa Y, Nozawa K, Nishibori M, Yamamoto Y, Okabayashi H, Tsunoda K, Yamagata T, Suzuki Y, Kinoshita K, Maeda Y, Nyunt M N, Daing T, Hla T, Win N, Tur T, Aung P, Cho A. Cytogenetic analysis of mithan in myanmar. Report of the Society for Researches on Native Livestock, 2004, 21: 123-127.

[29]Namikawa T, Amano T, Okada I, Hasnath M A. Blood groups and genetic variants of blood protein-enzymes in the Bangladesh native

 

cattle and gayals. Report of the Society for Researches on Native Livestock, 1988, 12: 77-88.

[30]Nie L, Yu Y, Zhang X Q, Yang G F, Wen J K, Zhang Y P. Genetic diversity of cattle in south China as revealed by blood protein electrophoresis. Biochemical Genetics, 1999, 37(7/8): 257-265.

[31]Tu Z C, Nie L, Yu Y, Wen J K, Zhang Y P. Blood protein polymorphism in B.frontalis, B.grunniens, B.taurus, and B.indicus. Biochemical Genetics, 2000, 38(11/12): 413-416.

[32]Nomura K, Takahashi Y, Amano T, Yamagata T, Mannen H, Nishibori M, Yamamoto Y, Kurosawa Y, Tanaka K, Okabayashi H, Tsunoda K, Nozawa K, Nyunt M M , Hla T, Win N, Maeda Y. Composition of genes controlling blood protein types of native cattle and mithan in Myanmar and their phylogenetic study. Report of the Society for Researches on Native Livestock, 2004, 21: 77-89.

[33]Ma G L, Chang H, Li S P, Chen H Y, Ji D J, Geng R Q, Chang C F, Li Y H. Phylogenetic relationships and status quo of colonies for gayal based on analysis of cytochrome b gene partial sequences. Journal of Genetics and Genomics, 2007, 34(5): 413-419.

[34]Dorji T, Mannen H, Namikawa T, Inamura T, Kawamoto Y. Diversity and phylogeny of mitochondrial DNA isolated from mithun Bos frontalis located in Bhutan. Animal Genetics, 2010, 41: 554-556.

[35]Tanaka K, Takizawa T, Murakoshi H, Dorji T, Nyunt M M, Maeda Y, Yamamoto Y, Namikawa T. Molecular phylogeny and diversity of Myanmar and Bhutan mithun based on mtDNA sequences. Animal Science Journal, 2010. doi: 10.1111/j.1740-0929.2010.00819.x.
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