Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (19): 4081-4087.doi: 10.3864/j.issn.0578-1752.2011.19.019

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Phylogenetic Relationships Among Domestic Chinese Bovinae Species Based on Mitochondrial Cytochrome b Gene Sequences

 GENG  Rong-Qing, WANG  Lan-Ping, JI  De-Jun, CHANG  Hong, LI  Yong-Hong, CHANG  Chun-Fang   

  1. 1.盐城师范学院生命科学与技术学院
    2.扬州大学动物科学与技术学院
  • Received:2010-08-20 Online:2011-10-01 Published:2010-12-30

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Abstract: 【Objective】This study was designed to analyze the phylogenetic relationships among domestic Chinese Bovinae species and provide molecular evidence for phylogenetic evolvement.【Method】The complete sequences of mitochondrial cytochrome b gene from six bovine species, including Bos taurus, Bos indicus, Bos grunniens, Bos frontalis, Bubalus bubalis (swamp buffalo) and Bos bubalis (river buffalo), were obtained by PCR and standard double-strand DNA sequencing method. The phylogenetic relationships among different bovine species were analyzed through molecular evolution software.【Result】The complete sequences of mitochondrial cytochrome b gene from six bovine species were all 1 140 bp in length. No insert and deletion mutation was observed. Total 220 variation sites were tested including 213 parsimony informative sites. The number of transition was higher than transversion and the ratio was 5.2, suggesting that the mutation was not saturation. Genetic distances showed that Bos taurus and Bos indicus possessed the most close relationship and the relationships between Bos grunniens, Bos frontalis, swamp water buffalo, river water buffalo and them deduced in turn gradually. Six bovine species distributed four main monophyletic branches and could be divided into Bos, Poephagus, Bibos, Bubalus, respectively. Divergency times among bovine species were between 0.775-6.43 MYA. The longest divergency time was between Asian water buffalo and other bovine species. The shortest divergency time was between Bos taurus and Bos indicus. The monophyletic relationship was not emerged between Bos frontalis and Bos gaurus, Bos frontalis and Bos gaurus did not differentiated clearly on mitochondrial DNA.【Conclusion】Six domestic Chinese Bovinae species could be divided into four branches corresponding to Bos, Poephagus, Bibos and Bubalus, respectively. The relationships between Bos and Poephagus, Bibos, Bubalus deduced in turn gradually. Divergency times among six bovine species were between 0.775-6.43 MYA. There was very close relationship between Bos frontalis and Bos gaurus, which showed that they might share the same maternal origin in an anterior time. The results did not support the opinion that Bos frontalis might be the domesticated form of Bos gaurus, Bos frontalis and Bos gaurus might be the progeny of a kind of extinct wild bovine species.

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