Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (19): 4022-4028.doi: 10.3864/j.issn.0578-1752.2011.19.012

• HORTICULTURE • Previous Articles     Next Articles

Effects of JA Synthesis-Related Genes Spr2 and LePrs on the

 ZHANG  Li-Ning, CHENG  Ji-Hong, YANG  Rui, SUN  Zhong-Hua, WU  Chun-Xia, WANG  Shao-Hui   

  1. 1.北京农学院植物科学技术学院/农业应用新技术北京市重点实验室
  • Received:2010-09-10 Online:2011-10-01 Published:2011-06-01

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Abstract: 【Objective】 Spr2 and LePrs were related with JA synthesis, the effects of the two genes on the response of tomato to root-knot nematodes were studied. 【Method】 In this paper, JA biosynthetic mutant (spr2 plants) and JA-overexpression transgenic plants (35S::PS plants) of tomato as well as wild tomato species (CM) were used, and the effects of MeJA sprays and grafting on root-knot numbers, PI-II content and Spr2, LePrs transcription levels, before and after inoculating root-knot nematodes, were analyzed. 【Result】 Compared with CM and 35S::PS plants, spr2  plants was more susceptible to root-knot nematodes. Spraying MeJA on tomato leaves could reduce root-knot numbers. Using 35S::PS plants as stock improved grafting tomato resistance to root-knot nematodes. 【Conclusion】 Resistance to nematodes in JA biosynthetic mutant (with mutant Spr2) was reduced, while in JA-overexpression transgenic tomato (with over-expressing LePrs) it was increased. So Jasmonic acid played a role in the response of tomato to root-knot nematodes.

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