Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (17): 3658-3665.doi: 10.3864/j.issn.0578-1752.2011.17.019

• VETERINARY SCIENCE • Previous Articles     Next Articles

Analysis of Fatty Acids Composition in the Liver of Mice with Non-alcoholic Fatty Liver Disease

WANG  Xin, CAO  Yu-Zhen, GUO  Gui-Fang, LI  Rui, ZHANG  Xiu-Ying   

  1. 1. 黑龙江八一农垦大学动物科技学院
    2. 东北农业大学动物医学院
  • Received:2010-12-20 Revised:2011-04-09 Online:2011-09-01 Published:2011-05-20

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Abstract: 【Objective】 The aim of this study was to determine the liver fatty acid composition of an experimental mouse model of NAFLD induced by high-fat feed and CCl4 and to assess the association between the change of liver fatty acids content and NAFLD. 【Method】C57BL/6J mice were given high-fat feed for six consecutive weeks to develop experimental NAFLD. Meanwhile, these mice were given subcutaneous injections of a 40% CCl4-vegetable oil mixture twice per week. Fatty acids in mice liver were extracted with diethyl and derived by BF3/methanol, then analyzed by gas chromatography-mass spectrometry (GC-MS) in selected ion monitoring. Qualitative analysis of fatty acids in mice liver was carried out according to the retrieval mass spectrometry database and the retention time of fatty acid standard. Quantitative analysis was determined by external standard method. 【Result】Pathological examination showed that NAFLD of C57BL/6J mice was successfully induced. Compared with the control group, high-fat feed and CCl4 led to significant increases in C14:0, C16:0, C18:0 and C20:3 (P＜0.01), and decreases in C15:0, C18:1, C18:2 and C18:3 (n-3) (P＜0.01) in the mouse liver. The treatment also led to an increase in saturated fatty acids (SFA) and decreases in other types fatty acids. An increase in the ratio of product/precursor n-6 (C20:4/C18:2) and n-3 [(C20:5+C22:6)/C18:3 (n-3)] and a decrease in the ratio of n-6/n-3 [C20:4/(C20:5+C22:6)] were also observed.【Conclusion】An increase of SFA, a decrease of unsaturated fatty acids (UFA), and metabolic disturbance of n-6 polyunsaturated fatty acids (PUFA) and n-3 PUFA in the liver of mice with NAFLD induced by high-fat feed and CCl4 were not only the results of NAFLD, but also the stimulating factors for the development of NAFLD.

Key words: Fatty acids, non-alcoholic fatty liver disease, mice, gas chromatography-mass spectrometry

 [1]Marchesini G, Bugianesi E, Forlani G, Cerrelli F, Lenzi M, Manini R, Natale S, Vanni E, Villanova N, Melchionda N, Rizzetto M. Nonalcoholic fatty liver, steatohepatitis, and the metabolic syndrome. Hepatology, 2003, 37(4): 917-923.

[2]Erickson S K. Nonalcoholic fatty liver disease. Journal of Lipid Research, 2009, 50: S412-S416.

[3]钱 林,胡小宣. 非酒精性脂肪肝分子发病机制的研究进展. 世界华人消化杂志, 2008, 16(25): 2848-2852.

Qian L, Hu X X. Advance in the molecular pathogenesis of nonalcoholic fatty liver disease. World Chinese Journal of Digestology, 2008, 16(25): 2848-2852. (in Chinese)

[4]Li Z Z, Berk M, McIntyre T M, Gores G J, Feldstein A E. The lysosomal-mitochondrial axis in free fatty acid-induced hepatic lipotoxicity. Hepatology, 2008, 47(5): 1495-1503.

[5]Joshi-Barve S, Barve S S, Amancherla K, Gobejishvili L, Hill D, Cave M, Hote P, McClain C J. Palmitic acid induces production of proinflammatory cytokine interleukin-8 from hepatocytes. Hepatology, 2007, 46(3): 823-830.

[6]Kohjima M, Enjoji M, Higuchi N, Kato M, Kotoh K, Yoshimoto T, Fujino T, Yada M, Yada R, Harada N, Takayanagi R, Nakamuta M. Re-evaluation of fatty acid metabolism-related gene expression in nonalcoholic fatty liver disease. International Journal of Molecular Medicine, 2007, 20(3): 351-358.

[7]阎 明, 孟繁立, 吕瑞娟, 贾晓青. 四氯化碳性脂肪肝血清游离脂肪酸谱变化. 中国病理生理杂志, 2004, 20(8): 1485-1488.

Yan M, Meng F L, Lv R F, Jia X Q. Alteration of serum free fatty acids during the carbon tetrachloride- induced fatty liver in rats. Chinese Journal of Pathophysiology, 2004, 20(8): 1485-1488. (in Chinese)

[8]DeAlmeida I T, Cortez-Pinto H, Fidalgo G, Rodrigues D, Camilo M E. Plasma total and free fatty acids composition in human non-alcoholic steatohepatitis. Clinical Nutrition, 2002, 21(3): 219-223.

[9]Dixon J B, Bhathal P S, Hughes N R, O’Brien P E. Nonalcoholic fatty liver disease：Improvement in liver histological analysis with weight loss. Hepatology, 2004, 39(6): 1647-1654．

[10]Brunt E M, Tiniakos D G. Histopathology of nonalcoholic fatty liver disease. World Journal of Gastroenterology，2010, 16(42): 5286-5296.

[11]Schwimmer J B, Behling C, Newbury R, Deutsch R, Nievergelt C, Schork N J, Lavine J E. Histopathology of pediatric nonalcoholic fatty liver disease. Hepatology, 2005, 42(3): 641-649.

[12]Soares M, Lopez-Bote C J. Effects of dietary lecithin and fat unsaturation on nutrient utilisation in weaned piglets. Animal Feed Science and Technology, 2002, 95(3-4): 169-177.

[13]Choi J S, Kim H, Jung M H, Hong S, Song J. Consumption of barley beta-glucan ameliorates fatty liver and insulin resistance in mice fed a high-fat diet. Molecular Nutrition & Food Research, 2010, 54(7): 1004-1013.

[14]Kennedy A, Martinez K, Chuang C C, LaPoint K, McIntosh M. Saturated fatty acid-mediated inflammation and insulin resistance in adipose tissue: Mechanisms of action and implications. The Journal of Nutrition, 2009, 139(1): 1-4.

[15]Weber LW, Boll M, Stampfl A. Hepatotoxicity and mechanism of haloalkane: carbon tetrachloride as a toxicological model. Critical Reviews in Toxicology, 2003, 33(2): 105-136.

[16]Yao D, Shi W, Gou Y, Zhou X, Yee A T, Zhou Y, Liu Z. Fatty acid-mediated intracellular iron translocation: a synergistic mechanism of oxidative injury. Free Radical Biology & Medicine, 2005, 39(10): 1385-1398.

[17]Barreyro FJ, Kobayashi S, Bronk S F, Werneburg N W, Malhi H, Gores G J. Transcriptional regulation of Bim by FoxO3A mediates hepatocyte lipoapoptosis. Journal of Biolological Chemistry, 2007, 282(37): 27141-27154.

[18]Puri P, Baillie R A, Wiest M M, Mirshahi F, Choudhury J, Cheung O, Sargeant C, Contos M J, Sanyal A J. A lipidomic analysis of nonalcoholic fatty liver disease. Hepatology, 2007, 46(4): 1081-1090.

[19]Rizki G, Arnaboldi L, Gabrielli B, Yan J, Lee G S, Ng R K, Turner S M, Badger T M, Pitas R E, Maher J J. Mice fed a lipogenic methionine-choline-deficient diet develop hypermetabolism coincident with suppression of SCD-1. Journal Lipid Research, 2006, 47: 2280-2290.

[20]杨丽丽, 柳  涛, 王  淼, 邢练军, 季  光, 郑培永. 氧化应激与非酒精性脂肪性肝病研究进展. 世界华人消化杂志, 2009, 17(9): 896-900.

Yang L L, Liu T, Wang M, Xing L J, Ji G, Zheng P Y. Progress in research of oxidative stress and nonalcoholic fatty liver disease. World Chinese Journal of Digestology, 2009, 17(9): 896-900. (in Chinese)

[21]陆金来, 陈金联, 陈明祥, 洪 静, 陈维雄, 朱金水, 陈尼维. 肝脏脂肪酸在高脂饮食大鼠非酒精性脂肪性肝病模型中的代谢. 世界华人消化杂志, 2008, 16(16): 1728-1733.

Lu J L, Chen J L, Chen M X, Hong J, Chen W W, Zhu J S, Chen N W. Fatty acids metabolic profile in high fat diet-induced nonalcoholic fatty liver disease in rats. World Chinese Journal of Digestology, 2008, 16(16): 1728-1733. (in Chinese)

[22]Buang Y, Cha J Y, Nagao K, Wang Y M, Inoue N, Yanagita T. Alleviation of fatty liver by alpha-linolenic acid. Journal Nutritional Science and Vitaminology, 2004, 50(4): 272-276.

[23]Videla L A, Rodrigo R, Araya J, Poniachik J. Oxidative stress and depletion of hepatic long-chain polyunsaturated fatty acids may contribute to nonalcoholic fatty liver disease. Free Radical Biology and Medicine, 2004, 37(9): 1499-1507.

[24]Clore J N, Stillman J S, Li J, O'Keefe S J, Levy J R. Differential effect of saturated and polyunsaturated fatty acids on hepatic glucose metabolism in humans. Americal Journal of Physiology- Endocrinology and Metabolism, 2004, 287(2): E358-E365.

[25]Flachs P, Rossmeisl M, Bryhn M, Kopecky J. Cellular and molecular effects of n-3 polyunsaturated fatty acids on adipose tissue biology and metabolism. Clinical Science, 2009, 116(1): 1-16.

[26]Capanni M, Calella F, Biagini M R, Genise S, Raimondi L, Bedogni G, Svegliati-Baroni G, Sofi F, Milani S, Abbate R, Surrenti C, Casini A. Prolonged n-3 polyunsaturated fatty acid supplementation ameliorates hepatic steatosis in patients with non-alcohoic fatty liver disease: a pilot study. Alimentary Pharmacology & Therapeutics, 2006, 23(8): 1143-1151.

[27]Levy J R, Clore J N, Stevens W. Dietary n-3 polyunsaturated fatty acid decrease hepatic triglycerides in fischer 344 rats. Hepatology, 2004, 39(3): 608-616.

[28]El-Badry A M, Graf R, Clavien P A. Omega 3-Omega 6: What is right for the liver? Hepatology, 2007, 47: 718-725.

[29]Chapkin R S, Davidson L A, Ly L, Weeks B R, Lupton J R, McMurray D N. Immunomodulatory effects of (n-3) fatty acids: putative link to inflammation and colon cancer. Journal Nutrition, 2007, 137: 200-204.

[30]Yonezawa Y, Hada T, Uryu K, Tsuzuki T, Eitsuka T, Miyazawa T, Murakami-Nakai C, Yoshida H, Mizushina Y. Inhibitory effect of conjugated eicosapentaenoic acid on mammalian DNA polymerase and topoisomerase activities and human cancer cell proliferation. Biochemical Pharmacology, 2005, 70: 453-460.

[31]Larter C Z, Yeh M M, Haigh W G, Williams J, Brown S, Bell-Anderson K S, Lee S P, Farrell G C. Hepatic free fatty acids accumulate in experimental steatohepatitis: Role of adaptive pathways. Journal of Hepatology, 2008, 48: 638-647.

[32]Araya J, Rodrigo R, Videla L A, Thielemann L, Orellana M, Pettinelli P, Poniachik J. Increase in long-chain polyunsaturated fatty acid n-6/n-3 ratio in relation to hepatic steatosis in patients with non-alcoholic fatty liver disease. Clinical Science, 2004, 106(6): 635-643.

[33]Tallman D L, Taylor C G. Effects of dietary fat and zinc on adiposity, serum leptin and adipose fatty acid composition in C57BL/6J mice. Journal of Nutritional Biochemistry, 2003, 14(1): 17-23.
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