中国荷斯坦牛FADS2基因3′端SNP突变对乳中脂肪酸组成的影响

doi:10.3864/j.issn.0578-1752.2016.11.015

Abstract

Abstract: 【Objective】D-6-fatty acid desaturase 2 (FADS2) is one of the key limiting enzymes in the conversion of dietary essential 18 carbon PUFAs (18C-PUFAs) such as linoleic acid (LNA,C18:2n-6) to γ-linolenic acid (GLA, 18:3n6), eicosapentaenoic acid (EPA, C20:5n3) and docosahexaenoic acid (DHA, C22:6n3). The objective of this study was to investigate the effects of SNPs in the 3’ untranslated regions of FADS2 gene on the compositions of fatty acids in milk of Chinese Holstein.【Method】In this study, 20 Chinese Holstein cows were selected randomly for PCR amplification and sequencing of the 3’ untranslated regions of FADS2 gene used for SNP discovery. Then the Chinese Holstein cows (n =551) were genotyped using Sequenom MassARRAY (Sequenom Inc., San Diego, CA) based on the previous SNP information in this study, and the associations between SNPs or haplotypes and compositions of fatty acids, unsaturated indexes of fatty acids in milk were analyzed by the least squares method in the GLM procedure of SPSS.【Result】Three SNPs (c.1571 A＞G, c.2743 A＞G and c.2776 A＞G) were identified in the 3’ untranslated regions of FADS2 gene. The genotype GG was the dominant genotype, which the frequencies were 0.800 for c.1571 A＞G, allele G was dominant allele with the frequency of 0.887. The genotype AA was the dominant genotype, which the frequencies were 0.673 for c. 2776 A＞G. The allele A was dominant allele with the frequency of 0.819. The coefficient of disequilibrium was 0.028 between c.1571 A＞G and c.2776 A＞G, which was not significant at P＜0.05. There were 3 haplotypes for FADS2 c.1571 A＞G and c.2776 A＞G, which the frequencies were 0.705, 0.181 and 0.114 for GA, GG and AA, respectively. χ² test indicated that the c.2776 A＞G fitted with Hardy-Weinberg equilibrium in the population (P＞0.05), and the c.1571 A＞G deviated from Hardy-Weinberg equilibrium (P＜0.05). The SNP c.1571 A＞G showed a very significant association with C14:1, unsaturated indexes of C14 and C18 (P＜0.01), and significant association with C18:0, SFA and MUFA (P＜0.05). The individuals with genotype GG had higher C14:1, unsaturated indexes of C14 and C18 than genotypes AG (P＜0.05). The SNP c.2776 A＞G showed a very significant association with C16:1, unsaturated indexes of C16 and C20 (P＜0.01), and significant association with C14:1 (P＜0.05). The individuals with genotype GG had higher C16:1, unsaturated indexes of C16 and C20 than genotypes AG and AA (P＜0.05). In the meantime, the haplotype of FADS2 1571-2776 showed a significant association with C16:1, unsaturated indexes of C20 (P＜0.05). The individuals with genotype GG had higher C16:1, unsaturated indexes of C20 than genotypes GA and AA (P＜0.05). 【Conclusion】The SNPs in the 3’ untranslated regions of FADS2 gene have significant genetic effects on composition of fatty acids in milk, but further investigation will be required to elucidate the biological and practical relevance of these SNPs.

Key words: Chinese Holstein, FADS2, SNPs, composition of fatty acids

XU Chen-xi, WANG Meng-qi, ZHU Xiao-rui, ZHANG Yu-feng, XIA Hai-lei, LIU Xian-hui, WANG Xiao-long, ZHANG Hui-min, YANG Zhang-ping, MAO Yong-jiang. Effects of SNPs in the 3’ Untranslated Regions of FADS2 on the Composition of Fatty Acids in Milk of Chinese Holstein[J].Scientia Agricultura Sinica, 2016, 49(11): 2194-2202.

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Effects of SNPs in the 3’ Untranslated Regions of FADS2 on the Composition of Fatty Acids in Milk of Chinese Holstein

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