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The Rate of Seed Contamination and Transmission of Cucumber green mottle mosaic virus in Watermelon and Melon

WU Hui-jie; QIN Bi-xia; CHEN Hong-yun; PENG Bin;CAI Jian-he; GU Qin-sheng   

  1. 1、Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009;
    2、Plant Protection Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007;
    3、Xiamen Entry-Exit Inspection and Quarantine Bureau,Xiamen 361026, Fujian
  • Received:2010-05-19 Online:2011-04-02 Published:2010-12-27

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Abstract: 【Objective】The objective of this study is to strengthen detection of seeds which carry virus, and to prevent the spread and prevalence of Cucumber green mottle mosaic virus (CGMMV). 【Method】 The rate of contamination and seed-to-seedling transmission of CGMMV in watermelon and melon were evaluated by DAS-ELISA. The seeds detected in the study were harvested from fruits of infected watermelon and melon plants by inoculation in insect-proof plastic house. 【Result】 The results showed that the contamination rate and seed-to-seedling transmission rate of CGMMV in watermelon were 100% and 2.25%, respectively, by testing 250 seeds and 623 seedlings. The contamination rate and seed-to-seedling transmission rate of melon was 93.85% and 2.83%, respectively, by analyzing 130 melon seeds and 2 050 melon seedlings. In the mixtures of CGMMV infected/ healthy seeds or leaves, the sensitivity was successfully detected at ratios of 1/1 000 and 1/10 000, respectively. 【Conclusion】The contamination rate was higher than the seed-to-seedling transmission rate, which showed that the virus distributes mainly in the seed coat. The sensitivity detection indicated that DAS-ELISA could be used to detect a large number of the seeds.

Key words: Cucumber green mottle mosaic virus (CGMMV) , watermelon , melon , seed contamination rate , seed-to-seedling transmission rate

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