Scientia Agricultura Sinica ›› 2010, Vol. 43 ›› Issue (7): 1524-1530 .doi: 10.3864/j.issn.0578-1752.2010.07.026

• RESEARCH NOTES • Previous Articles     Next Articles

Isolation of Microbial Strains Producing Acidic Xylanase and Characterization of the Enzyme

GUO Chun-yan, DIAO Qi-yu, QIAO Yu, TU Yan, ZHANG Nai-feng, JIANG Cheng-gang   

  1. （中国农业科学院饲料研究所）
  • Received:2009-04-01 Revised:2009-10-20 Online:2010-04-01 Published:2010-04-01
  • Contact: DIAO Qi-yu

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Abstract:

【Objective】 This study was aiming at screening acidic xylanase-producing microbes from natural environment for potential use in feed industry. 【Method】 Eighteen xylanase-producing strains were isolated from soil by enrichment culture techniques. Five strains were chosen to ferment in shaking flasks. Strain S8-3, which showed the highest xylanase production, was preliminarily identified by standard methods, and the property of the xylanase produced by the strain was studied. 【Result】 Strain S8-3 was identified as Aspergillus niger. The maximal enzymatic activity of xylanase in culture solution was 628.43 U•mL-1. The xylanase activity preformed optimally at 45℃ and pH 4.5. The xylanase was stable at pH ranging from 3.0 to 7.0 and retained more than 60 % of its original activity after incubation for 30 min at 70℃. 【Conclusion】 The xylanase produced by strain S8-3 in this study may have the potential in feed industry.

Key words: feed, xylanase, Aspergillus niger, enzymatic properties

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