Abstract:

【Objective】 The aim of the study was to understand and find differential proteins induced by PP333, which could provide valuable information for further study on its effective law and mechanism. 【Method】 Two-dimensional electrophoresis (2-DE) was used to study the variation expression of proteins of cucumber cotyledonary nodes induced with PP333. The characteristics and functions of differentially expressed proteins were analyzed with MALDI-TOF-TOF/MS and Protein Data Bank. 【Result】 Nearly 1 000 proteins were reproducibly detected in 2-DE gel. Five differential proteins were identified, including 14-3-3 protein (R2), aminopeptidase (R7), methionine aminopeptidase (R11), 60s acidic ribosomal protein (R20) and PEPC (R21). The proteins were closely related to metabolism, translation and signal transduction, which may play an important role in flower bud differentiation of cucumber. 【Conclusion】 PP333 treatment induced the variation of many proteins which regulated plant growth and development by their mutual coordination.

Key words: PP333, cucumber cotyledonary node, differential protein, mass spectrum