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| Development of SSR Markers for a Phytopathogenic Fungus, Blumeria graminis f.sp. tritici, Using a FIASCO Protocol |
| WANGMeng123, XUEFei4, YANGPeng15, DUANXia-yu1, ZHOUYi-lin1, SHENChong-yao2, ZHANGGuo-zhen2, WANGBao-tong5 |
1.Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100093, P.R.China
2.Department of Plant Pathology, China Agricultural University/Key Laboratory of Plant Pathology, Ministry of Agriculture, Beijing 100193,P.R.China
3.Huai’an Entry-Exit Inspection and Quarantine Bureau, Jiangsu 223001, P.R.China
4.College of Agronomy, Shihezi University, Shihezi 832000, P.R.China
5.College of Plant Protection, Northwest A&F University, Yangling 712100, P.R.China |
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摘要 Simple sequence repeats (SSR) have been widely used as molecular markers due to their abundance and high polymorphism. However, up to now, the SSR markers had not been developed in the obligate biotrophic phytopathogenic fungus, Blumeria graminis f.sp. tritici. From (AC)10 and (AG)10 enriched genomic libraries for Bgt, 25 primer pairs were designed using the FIASCO (fast isolation by AFLP of sequences containing repeats) protocol. Five primer pairs exhibited polymorphism with allelic diversity from two to seven alleles and produced 29 alleles in a survey of 90 isolates collected from six provinces (cities) in China, while the others displayed monomorphic. Levels of observed heterozygosity ranged from 0.000-0.044 (mean 0.025) and expected heterozygosity ranged from 0.297-0.816 (mean 0.538). These molecular markers provide a novel source to genetic diversity assays and to genetic and physical mapping of Bgt. SSR markers of Bgt need to be further explored.
Abstract Simple sequence repeats (SSR) have been widely used as molecular markers due to their abundance and high polymorphism. However, up to now, the SSR markers had not been developed in the obligate biotrophic phytopathogenic fungus, Blumeria graminis f.sp. tritici. From (AC)10 and (AG)10 enriched genomic libraries for Bgt, 25 primer pairs were designed using the FIASCO (fast isolation by AFLP of sequences containing repeats) protocol. Five primer pairs exhibited polymorphism with allelic diversity from two to seven alleles and produced 29 alleles in a survey of 90 isolates collected from six provinces (cities) in China, while the others displayed monomorphic. Levels of observed heterozygosity ranged from 0.000-0.044 (mean 0.025) and expected heterozygosity ranged from 0.297-0.816 (mean 0.538). These molecular markers provide a novel source to genetic diversity assays and to genetic and physical mapping of Bgt. SSR markers of Bgt need to be further explored.
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Received: 31 January 2013
Accepted:
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| Fund: This work was supported by the National Basic Research Program of China (2006CB100203 and 2011CB100403), the Key Technologies R&D Program of China during the 11th Five-Year Plan period (2006BAD08A05) and the Special Fund for Agro-Scientific Research in the Public Interest (3- 15), China. |
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Corresponding Authors:
DUAN Xia-yu, Tel: +86-10-62815946, Fax: +86-10-62815946, E-mail: xyduan@ippcaas.cn
E-mail: xyduan@ippcaas.cn
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| About author: WANG Meng, E-mail: wangmeng1019xiao@gmail.com |
Cite this article:
WANGMeng123 , XUEFei4 , YANGPeng15 , DUANXia-yu1 , ZHOUYi-lin1 , SHENChong-yao2 , ZHANGGuo-zhen2 , WANGBao-tong5 .
2014.
Development of SSR Markers for a Phytopathogenic Fungus, Blumeria graminis f.sp. tritici, Using a FIASCO Protocol. Journal of Integrative Agriculture, 13(1): 100-104.
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