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Journal of Integrative Agriculture  2023, Vol. 22 Issue (8): 2456-2469    DOI: 10.1016/j.jia.2022.08.025
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Population genetic variation and historical dynamics of the natural enemy insect Propylea japonica (Coleoptera: Coccinellidae) in China

WANG Meng-qi1, 2, 3, ZHANG Hong-rui2, XI Yu-qiang1, 4, WANG Gao-ping1, 4, ZHAO Man1, 4, ZHANG Li-juan1, 4#, GUO Xian-ru1,4#

1 Department of Entomology, College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, P.R.China

2 College of Plant Protection, Yunnan Agricultural University, Kunming 650201, P.R.China

3 Suifenhe Bureau of Agriculture and Rural Affairs, Suifenhe 157300, P.R.China

4 Henan International Laboratory for Green Pest Control/College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, P.R.China

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摘要  

龟纹瓢虫(鞘翅目:瓢虫科)在中国是一种自然天敌昆虫,捕食范围广泛,常用于害虫治理。然而其在中国区域内遗传模式(遗传变异、种群结构和历史动态)还不清楚,从而阻碍了害虫生物防治的发展进程。种群遗传数据对于天敌生防过程中不同阶段策略的优化具有很大潜力。本研究通过收集中国区域内30个采样区域的462头龟纹瓢虫样本,采用23对微卫星和线粒体COI分子标记,开展了该种的群遗传工作。微卫星数据显示龟纹瓢虫具有中等水平的遗传多样性,线粒体基因则显示出高水平的遗传多样性。与长江流域种群相比,黄河流域种群具有更高的遗传分歧。龟纹瓢虫中国种群未形成显著的地理种群结构,但存在群体分化的迹象,可能与种群间频繁的基因交流有关。种群经历瓶颈后出现扩张,寄主植物——害虫——天敌之间的三级营养关系是种群扩张的重要因素。种群遗传研究在害虫生物防治过程中发挥着重要作用,本研究通过估测种群遗传多样性、种群遗传差异和推种群历史动态,为有效利用天敌昆虫提供重要的遗传信息。



Abstract  

Propylea japonica (Coleoptera: Coccinellidae) is a natural enemy insect with a wide range of predation in Chinese mainland and is commonly used in pest management.  However, its genetic pattern (i.e., genetic variation, genetic structure, and historical population dynamics) is still unclear, impeding the development of biological control of insect pests.  Population genetic research has the potential to optimize strategies at different stages of the biological control processes.  This study used 23 nuclear microsatellite sites and mitochondrial COI genes to investigate the population genetics of Propylea japonica based on 462 specimens collected from 30 sampling sites in China.  The microsatellite dataset showed a moderate level of genetic diversity, but the mitochondrial genes showed a high level of genetic diversity.  Populations from the Yellow River basin were more genetically diverse than those in the Yangtze River basin.  Propylea japonica has not yet formed a significant genealogical structure in China, but there was a population structure signal to some extent, which may be caused by frequent gene flow between populations.  The species has experienced population expansion after a bottleneck, potentially thanks to the tri-trophic plant–insect–natural enemy relationship.  Knowledge of population genetics is of importance in using predators to control pests.  Our study complements existing knowledge of an important natural predator in agroecosystems through estimating its genetic diversity and population differentiation and speculating about historical dynamics.

Keywords:  demographic history              genetic differentiation              genetic diversity              population expansion              microsatellite              mitochondrial COI  
Received: 12 February 2022   Accepted: 16 May 2022
Fund: This work was supported by a grant from the Key S&T Special Project of Henan, China (201300111500), the National Key R&D Program of China (2018YFD0200600), the Modern Agricultural System in Industry Technology of Henan Province, China (S2015-02-G05), the Key R&D and Promotion Project in Henan Province, China (212102110471).  


About author:  WANG Meng-qi, E-mail: even0827@126.com; #Correspondence ZHANG Li-juan, E-mail: zhlj042@126.com; GUO Xian-ru, E-mail: guoxianru@126.com

Cite this article: 

WANG Meng-qi, ZHANG Hong-rui, XI Yu-qiang, WANG Gao-ping, ZHAO Man, ZHANG Li-juan, GUO Xian-ru. 2023. Population genetic variation and historical dynamics of the natural enemy insect Propylea japonica (Coleoptera: Coccinellidae) in China. Journal of Integrative Agriculture, 22(8): 2456-2469.

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