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Journal of Integrative Agriculture  2020, Vol. 19 Issue (2): 350-359    DOI: 10.1016/S2095-3119(19)62600-6
Section 2 Invasive insect pests of potato: global perspectives Advanced Online Publication | Current Issue | Archive | Adv Search |
Genetic structure of the invasive Colorado potato beetle Leptinotarsa decemlineata populations in China
YANG Fang-yuan1, 2, GUO Jian-jun1, LIU Ning2, ZHANG Run-zhi1, 2, 3
1 Institute of Entomology, Guizhou University, Guiyang 550025, P.R.China
2 Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, P.R.China
3 University of Chinese Academy of Sciences, Beijing 100049, P.R.China
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The Colorado potato beetle (CPB), Leptinotarsa decemlineata Say, is an infamous invasive species worldwide.  It was first found in Xinjiang Uygur Autonomous Region of China in 1993 and spread to Northeast China in 2013.  To better understand the genetic structure and the diffusion path of their populations in China, we used nine polymorphic microsatellite loci to elucidate the genetic diversity, genetic structure and gene flow among nine CPB populations across Xinjiang and Northeast China.  The results show that: (1) Two genetically separated clusters were identified by phylogenetic tree, principal coordinate analysis (PCoA) and Bayesian cluster method.  Cluster one contained populations from Xinjiang, China.  Cluster two contained populations from Northeast China.  A genetic differentiation existed between the two clusters.  (2) Three populations in Northeast China hold an obvious genetic differentiation according to the phylogenetic tree and PCoA, indicating that multiple introductions may occur in Northeast China.  (3) The Altay population in Xinjiang showed a closer genetic relationship with the populations in Northeast China which may be due to the fact that they collectively originated in neighboring Russia.  (4) Among all populations, Mulei and Wusu had obvious gene migrations from Tacheng, indicating that the inland populations are most likely to originate from Tacheng, Xinjiang.
Keywords:  Colorado potato beetle        genetic variation        alien invasive species        microsatellite marker  
Received: 30 August 2018   Accepted:
Fund: This research was supported by the Monitoring and Control Technology of Alien Species Program of the Ministry of Science and Technology, China (2015BAD08B03), the Strategic Priority Research Program of the Chinese Academy Sciences (CAS) (XDA19050204), the National Key Research and Development Program of China (2018YFD0200802), and the National Invasive Biological Prevention and Control Technology Program of the CAS (KFZD-SW-208-07).
Corresponding Authors:  Correspondence ZHANG Run-zhi, Tel: +86-10-64807270, E-mail:   
About author:  YANG Fang-yuan, Mobile: +86-17611313910, E-mail:;

Cite this article: 

YANG Fang-yuan, GUO Jian-jun, LIU Ning, ZHANG Run-zhi. 2020.

Genetic structure of the invasive Colorado potato beetle Leptinotarsa decemlineata populations in China
. Journal of Integrative Agriculture, 19(2): 350-359.

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