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Journal of Integrative Agriculture  2021, Vol. 20 Issue (5): 1277-1286    DOI: 10.1016/S2095-3119(20)63400-1
Special Issue: 园艺-分子生物合辑Horticulture — Genetics · Breeding
Horticulture Advanced Online Publication | Current Issue | Archive | Adv Search |
Fingerprinting 146 Chinese chestnut (Castanea mollissima Blume) accessions and selecting a core collection using SSR markers
NIE Xing-hua1, 2, WANG Ze-hua2, LIU Ning-wei2, SONG Li2, YAN Bo-qian2, XING Yu1, 2, ZHANG Qing2, FANG Ke-feng3, ZHAO Yong-lian4, CHEN Xin5, WANG Guang-peng6, QIN Ling1, 2, CAO Qing-qin1, 2
1 Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing University of Agriculture, Beijing 102206, P.R.China
2 College of Plant Science and Technology, Beijing Key Laboratory for Agricultural Application and New Technique, Beijing University of Agriculture, Beijing 102206, P.R.China
3 College of Landscape Architecture, Beijing University of Agriculture, Beijing 102206, P.R.China
4 Chestnut Technology Experiment and Extension Station of Huairou District, Beijing 102206, P.R.China
5 Shandong Institute of Pomology, Shandong Academy of Agricultural Sciences, Tai’an 271000, P.R.China
6 Changli Institute of Pomology, Hebei Academy of Agriculture and Forestry Sciences, Changli 066600, P.R.China
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Chinese chestnut is an important nut tree around the world.  Although the types of Chinese chestnut resources are abundant, resource utilization and protection of chestnut accessions are still very limited.  Here, we fingerprinted and determined the genetic relationships and core collections of Chinese chestnuts using 18 fluorescently labeled SSR markers generated from 146 chestnut accessions.  Our analyses showed that these markers from the tested accessions are highly polymorphic, with an average allele number (Na) and polymorphic information content (PIC) of 8.100 and 0.622 per locus, respectively.  Using these strongly distinguishing markers, we successfully constructed unique fingerprints for 146 chestnut accessions and selected seven of the SSR markers as core markers to rapidly distinguish different accessions.  Our exploration of the genetic relationships among the five cultivar groups indicated that Chinese chestnut accessions are divided into three regional type groups: group I (North China (NC) and Northwest China (NWC) cultivar groups), group II (middle and lower reaches of the Yangtze River (MLY) cultivar group) and group III (Southeast China (SEC) and Southwest China (SWC) cultivar groups).  Finally, we selected 45 core collection members which represent the most genetic diversity of Chinese chestnut accessions.  This study provides valuable information for identifying chestnut accessions and understanding the phylogenetic relationships among cultivar groups, which can serve as the basis for efficient breeding in the future.
Keywords:  Castanea mollissima        SSR markers        fingerprinting        phylogeny        core collections  
Received: 06 January 2020   Accepted:
Fund: This work was supported by grants from the Project of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges under Beijing Municipality, China (IDHT20180509); the National Key Research & Development Program of China (2018YFD1000605) and the Opening Project of Beijing Key Laboratory of New Technology in Agricultural Application, China (kf2018024).
Corresponding Authors:  Correspondence QIN Ling, Tel/Fax: +86-10-80797229, E-mail:; CAO Qing-qin, E-mail:    
About author:  NIE Xing-hua, E-mail:;

Cite this article: 

NIE Xing-hua, WANG Ze-hua, LIU Ning-wei, SONG Li, YAN Bo-qian, XING Yu, ZHANG Qing, FANG Ke-feng, ZHAO Yong-lian, CHEN Xin, WANG Guang-peng, QIN Ling, CAO Qing-qin. 2021. Fingerprinting 146 Chinese chestnut (Castanea mollissima Blume) accessions and selecting a core collection using SSR markers. Journal of Integrative Agriculture, 20(5): 1277-1286.

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