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| Fosfomycin Resistance in Avian Pathogenic Escherichia coli Isolates |
| JIN Wen-jie, ZHENG Zhi-ming, WANG Qian-qian, QIN Ai-jian, SHAO Hong-xia , QIAN Kun |
1.Key Lab for Avian Preventive Medicine, Ministry of Education/College of Veterinary Medicine, Yangzhou University, Yangzhou 225009,
P.R.China2.Circulation Industry Promotion Center, Ministry of Commerce, Beijing 100070, P.R.China
3.Animal Disease Preventive and Control Center of Yangzhou, Yangzhou 225009, P.R.China |
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摘要 Fosfomycin, a broad-spectrum antibiotic against both Gram-positive and Gram-negative bacteria, is very important in the clinic but many fosfomycin-resistant bacteria have been isolated from patients. In this study, the resistance mechanism of three fosfomycin-resistant avian pathogenic Escherichia coli (APEC) strains (JE1, IF7 and CD11) isolated from septicemic chickens were analyzed. The results showed that their fosfomycin-resistance mechanisms were different. An alteration in the glpT transport system was the main reason of the fosfomycin-resistance mechanisms of strain IF7. Compared with the control stain BL21, the capacity of fosfomycin-uptake was low in all these three stains (JE1>IF7>CD11). Sequence results of murA showed that there were more than 10 sites of nucleotide mutation, but only one amino acid mutation T116A showed in CD11. Real-time detection test showed that the expression level of the murA gene of the three stains was significantly increased (four times increase in strain CD11 and two times increase in strains JE1 and IF7). The transformation and recombinant test showed that the recombinant bacteria with the murA of JE1 and CD11 showed high minimal inhibitory concentration (MIC) against fosfomycin. From the results of this research, it showed that most of the fosfomycinresistance mechanisms once showed in patient bacteria have appeared in the APEC strains and the fosfomycin-resistance mechanism of the three APEC isolates was different.
Abstract Fosfomycin, a broad-spectrum antibiotic against both Gram-positive and Gram-negative bacteria, is very important in the clinic but many fosfomycin-resistant bacteria have been isolated from patients. In this study, the resistance mechanism of three fosfomycin-resistant avian pathogenic Escherichia coli (APEC) strains (JE1, IF7 and CD11) isolated from septicemic chickens were analyzed. The results showed that their fosfomycin-resistance mechanisms were different. An alteration in the glpT transport system was the main reason of the fosfomycin-resistance mechanisms of strain IF7. Compared with the control stain BL21, the capacity of fosfomycin-uptake was low in all these three stains (JE1>IF7>CD11). Sequence results of murA showed that there were more than 10 sites of nucleotide mutation, but only one amino acid mutation T116A showed in CD11. Real-time detection test showed that the expression level of the murA gene of the three stains was significantly increased (four times increase in strain CD11 and two times increase in strains JE1 and IF7). The transformation and recombinant test showed that the recombinant bacteria with the murA of JE1 and CD11 showed high minimal inhibitory concentration (MIC) against fosfomycin. From the results of this research, it showed that most of the fosfomycinresistance mechanisms once showed in patient bacteria have appeared in the APEC strains and the fosfomycin-resistance mechanism of the three APEC isolates was different.
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Received: 18 July 2012
Accepted:
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| Fund: This work was supported by the grants (30800822) from NSFC, the Innovative Research Funding of Yangzhou University, China (2011CXJ067), also sponsored by Qing Lan Project, the Program for Changjiang Scholars, the Innovative Research Team in University, Ministry of Education of China (IRT0978), and the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, China (PAPD). |
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Corresponding Authors:
Correspondence QIN Ai-jian, E-mail: aijian@yzu.edu.cn
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| About author: JIN Wen-jie, E-mail: wenjiejin1@163.com |
Cite this article:
JIN Wen-jie, ZHENG Zhi-ming, WANG Qian-qian, QIN Ai-jian, SHAO Hong-xia , QIAN Kun.
2012.
Fosfomycin Resistance in Avian Pathogenic Escherichia coli Isolates. Journal of Integrative Agriculture, 12(12): 2051-2057.
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