|
|
|
|
|
| Effect of Semen vaccariae and Taraxacumogono on CellAdhesion of Bovine Mammary Epithelial Cells |
| TONG Jin-jin, LI Ye, LIU Rong, GAO Xue-jun , LI Qing-zhang |
| Key Laboratory of Dairy Science, Ministry of Education/College of Life Science, Northeast Agricultural University, Harbin 150030, P.R. China |
|
|
|
摘要 The aim of this study is to reveal the regulation mechanism of the effect of Semen vaccariae and Taraxacu mogono on the cell-cell adhersion molecule, E-cadherin and b-catenin on the proliferation role and secretion function of bovine mammary epithelial cells cultured in vitro. Firstly, the epithelial character of bovine mammary epithelial cells was authenticated using immunofluorescence, then the cell grow curve was observed and investigated after S. vaccariae and T. mogono treatment. On the effect of S. vaccariae and T. mogono, cell adhesion molecules E-cadherin, b-catenin and CycinD1 mRNA and protein were detected by qRT-PCR and Western blotting, respectively. The results showed that the cellular keratin 18 expressed positively and proliferated vigorously after S. vaccariae and T. mogono treament. The mRNA and protein levels of E-cadherin and CycinD1 were remarkably higher (P<0.05) in 36 h after S. vaccariae and T. mogono treatment. The cell proliferation at 36 h was increased significantly (P<0.05). In conclusion, S. vaccariae and T. mogono have a positive impact on the cell proliferation and an effect on the adhesion molecules E-cadherin, b-catenin and CycinD1 in the Wnt signaling pathway.
Abstract The aim of this study is to reveal the regulation mechanism of the effect of Semen vaccariae and Taraxacu mogono on the cell-cell adhersion molecule, E-cadherin and b-catenin on the proliferation role and secretion function of bovine mammary epithelial cells cultured in vitro. Firstly, the epithelial character of bovine mammary epithelial cells was authenticated using immunofluorescence, then the cell grow curve was observed and investigated after S. vaccariae and T. mogono treatment. On the effect of S. vaccariae and T. mogono, cell adhesion molecules E-cadherin, b-catenin and CycinD1 mRNA and protein were detected by qRT-PCR and Western blotting, respectively. The results showed that the cellular keratin 18 expressed positively and proliferated vigorously after S. vaccariae and T. mogono treament. The mRNA and protein levels of E-cadherin and CycinD1 were remarkably higher (P<0.05) in 36 h after S. vaccariae and T. mogono treatment. The cell proliferation at 36 h was increased significantly (P<0.05). In conclusion, S. vaccariae and T. mogono have a positive impact on the cell proliferation and an effect on the adhesion molecules E-cadherin, b-catenin and CycinD1 in the Wnt signaling pathway.
|
|
Received: 27 June 2011
Accepted:
|
| Fund: This study was financially supported by the National Basic Research Program of China (2011CB100804). |
|
Corresponding Authors:
Correspondence LI Qing-zhang, E-mail: qingzhangli@hotmail.com
E-mail: qingzhangli@hotmail.com
|
| About author: TONG Jin-jin, E-mail: tongjinjin0451@163.com |
Cite this article:
TONG Jin-jin, LI Ye, LIU Rong, GAO Xue-jun , LI Qing-zhang.
2012.
Effect of Semen vaccariae and Taraxacumogono on CellAdhesion of Bovine Mammary Epithelial Cells. Journal of Integrative Agriculture, 12(12): 2043-2050.
|
| [1]Arduino P G, Carrozzo M, Pagano M, Broccoletti R, ScullyC, Gandolfo S. 2009. Immunohistochemical expressionof basement membrane proteins of verrucous carcinomaof the oral mucosa. Clinical Oral Investigations, 14,297-302.[2]Boras-Granic K, Chang H, Grosschedl R, Hamel PA. 2006.Lef1 is required for the transition of Wnt signaling frommesenchymal to epithelial cells in the mouse embryonicmammary gland. Developmental Biology, 295, 219-231.[3]Chu E Y, Hens J, Andl T, Kairo A, Yamaguchi T, Brisken C,GlickA,Wysolmerski J,Millar S E. 2004. CanonicalWNTsignaling promotes mammary placode development andis essential for initiation of mammary glandmorphogenesis. Development, 131, 4819-4829.[4]Dowgiert J, Sosne G, Kurpakus-Wheater M. 2004. Laminin-2 stimulates the proliferation of epithelial cells in aconjunctival epithelial cell line. Cell Proliferation, 37,161-175.[5]Faraldo M M, Teulière J, Deugnier M A, Birchmeier W,Huelsken J, Thiery J P, Cano A, Glukhova M A. 2007.Beta-catenin regulates P-cadherin expression inmammary basal epithelial cells. FEBS Letters, 581, 831-836.[6]Hakim S G, Kosmehl H, Sieg P, Trenkle T, Jacobsen H C,Benedek G A, Ribbat J, Driemel O. 2011. Alteredexpression of cell-cell adhesion molecules ?-catenin/Ecadherinand related Wnt-signaling pathway in sporadicand syndromal keratocystic odontogenic tumors.Clinical Oral Investigations, 15, 321-328.[7]Heuberger J, Birchmeier W. 2009. Interplay of cadherinmediatedcell adhesion and canonical Wnt signaling.Cold Spring Harb Perspect in Biology. [2011-07-19].http://cshperspectives.cshlp.org/content/2/2/a002915.(abstract)Hirohashi S, Kanai Y. 2003. Cell adhesion system and humancancer morphogenesis. Cancer Science, 94, 575-581.[8]Horsley V. 2009. Epigenetics, Wnt signaling, and stem cells:the Pygo2 connection. The Journal of Cell Biology,185, 761-763.[9]Kuphal F, Behrens J. 2006. E-cadherin modulates Wntdependenttranscription in colorectal cancer cells butdoes not alter Wnt-independent gene expression infibroblasts. Experimental Cell Research, 312, 457-467.[10]Lapyckyj L, Castillo L F, Matos M L, Gabrielli N M, LüthyI A, Vazquez-Levin M H. 2010. Expression analysis ofepithelial cadherin and related proteins in IBH-6 andIBH-4 human breast cancer cell lines. Journal of CellularPhysiology, 3, 596-605.[11]Morrison B, Cutler M L. 2010. The contribution of adhesions igna l ing to l actogenes is. Journal of Cel lCommunicatio and Signaling, 4, 131-139.[12]Nørgaard J V, Nielsen M O, Theil P K, Sørensen M T,Safayi S, Sejrsen K. 2008. Development of mammaryglands of fat sheep submitted to restricted feedingduring late pregnancy. Small Ruminant Research, 76,155-165.[13]Qin J, LI Q Z, Gao X J. 2008. Effect of main componentsfrom semen vaccariae on multiplication and expressionof beta-casein of mouse mammary epithelial cell in vitro.Scientia Agricultura Sinica, 8, 2442-2447. (in Chinese)[14]Rudolph M C, McManaman J L, Hunter L, Phang T, NevilleMC. 2003. Functional development of the mammarygland: use of expression profiling and trajectoryclustering to reveal changes in gene expression duringpregnancy, lactation, and involution. Journal ofMammary Gland Biology and Neoplasia, 8, 287-307.[15]Schmalhofer O, Brabletz S, Brabletz T. 2009. E-cadherin, b-catenin, and ZEB1 in malignant progression of cancer.Cancer Metastasis Reviews, 28, 151-166.[16]Su Y, Shankar K, Rahal O, Simmen R C. 2011. Bidirectionalsignaling of mammary epithelium and stroma:implications for breast cancer-preventive actions ofdietary factors. Journal of Nutritional Biochemistry,22, 605-611.[17]Su Y, Simmen R C. 2009. Soy isoflavone genisteinupregulates epithelial adhesion molecule E-cadherinexpression and attenuates beta-catenin signaling inmammary epithelial cells. Carcinogenesis, 30, 331-339,[18]Wan Z Y, Tong H L, Li Q Z, Gao X J. 2010. Influence on thespecific micrornas in bovine mammary gland epithelialcells by semen vaccariae active monomer and prolactin.China Animal Husbandry and Veterinary Medicine,8, 230-232.[19]Yang L J, Liu Y Q, Gu B, Bian X C, Feng H L, Yang Z L, LiuY Y. 2010. Effect of forced E-cadherin expression onadhesion and proliferation of human breast carcinomacells. Chinese Journal of Pathology, 12, 842-847. (in Chinese)[20]Zeljko M, Pecina-Slaus N, Martic T N, Kusec V, Beros V,Tomas D. 2011. Molecular alterations of E-cadherin andbeta-catenin in brain metastases. Frontiers inBioscience, 3, 616-624.[21]Zhang Y H, Yan W S, Chen X B. 2011. Mutant p53 disruptsMCF-10A cell polarity in three-dimensional culture viaepithelial-to-mesenchymal transitions. Journal ofBiological Chemistry, 286, 16218-16228. |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
