|
|
|
|
|
| Molecular Characterization and Expression Analysis of TaZFP15, a C2H2- Type Zinc Finger Transcription Factor Gene in Wheat (Triticum aestivum L.) |
| SUN Zhao-hua, DING Chang-huan, LI Xiao-juan , XIAO Kai |
1.College of Life Science, Agricultural University of Hebei, Baoding 071001, P.R.China
2.College of Agronomy, Agricultural University of Hebei, Baoding 071001, P.R.China |
|
|
|
摘要 Based on sequencing of part clones in a root subtractive cDNA library, an expressed sequence tag (EST) sharing high similarity to a rice C2H2 zinc finger transcription factor (ZFP15) was obtained in wheat. Through bioinformatics approach, the wheat C2H2-type ZFP gene referred to TaZFP15 has been identified and characterized. As a full-length cDNA of 670 bp, TaZFP15 has an open reading frame of 408 bp and encodes a 135-aa polypeptide. TaZFP15 contains two C2H2 zinc finger domains and each one has a conserved motif QALGGH. The typical L-box, generally identified in the C2H2 type transcription factors, has also been found in TaZFP15. Phylogenetic analysis suggested that TaZFP15 shares high similarities with rice ZFP15 (GenBank accession no. AY286473), maize ZFP (GenBank accession no. NM_001159094) and a subset of other zinc-finger transcription factor genes in plant species. The expression of TaZFP15 was up-regulated by starved-Pi stress, showing a pattern to be gradually elevated along with the progression of the Pi-stress in a 23-h treatment regime. Similarly, the transcripts of TaZFP15 in roots were also induced by nitrogen deficiency, and abiotic stresses of drought and salinity. No responses of TaZFP15 were detected in roots to nutrition deficiencies of P, Zn, and Ca, and the external treatment of abscisic acid (ABA). TaZFP15 could be specifically amplified in genome A, B, and D, and without variability in the sequences, suggesting that TaZFP15 has multi-copies in the homologous hexaploid species. Transgenic analysis in tobacco revealed that up-regulation of TaZFP15 could significantly improve plant dry mass accumulation via increasing the plant phosphorus acquisition capacity under Pi-deficiency condition. The results suggested that TaZFP15 is involved in mediation of signal transductions of diverse external stresses.
Abstract Based on sequencing of part clones in a root subtractive cDNA library, an expressed sequence tag (EST) sharing high similarity to a rice C2H2 zinc finger transcription factor (ZFP15) was obtained in wheat. Through bioinformatics approach, the wheat C2H2-type ZFP gene referred to TaZFP15 has been identified and characterized. As a full-length cDNA of 670 bp, TaZFP15 has an open reading frame of 408 bp and encodes a 135-aa polypeptide. TaZFP15 contains two C2H2 zinc finger domains and each one has a conserved motif QALGGH. The typical L-box, generally identified in the C2H2 type transcription factors, has also been found in TaZFP15. Phylogenetic analysis suggested that TaZFP15 shares high similarities with rice ZFP15 (GenBank accession no. AY286473), maize ZFP (GenBank accession no. NM_001159094) and a subset of other zinc-finger transcription factor genes in plant species. The expression of TaZFP15 was up-regulated by starved-Pi stress, showing a pattern to be gradually elevated along with the progression of the Pi-stress in a 23-h treatment regime. Similarly, the transcripts of TaZFP15 in roots were also induced by nitrogen deficiency, and abiotic stresses of drought and salinity. No responses of TaZFP15 were detected in roots to nutrition deficiencies of P, Zn, and Ca, and the external treatment of abscisic acid (ABA). TaZFP15 could be specifically amplified in genome A, B, and D, and without variability in the sequences, suggesting that TaZFP15 has multi-copies in the homologous hexaploid species. Transgenic analysis in tobacco revealed that up-regulation of TaZFP15 could significantly improve plant dry mass accumulation via increasing the plant phosphorus acquisition capacity under Pi-deficiency condition. The results suggested that TaZFP15 is involved in mediation of signal transductions of diverse external stresses.
|
|
Received: 15 January 2011
Accepted:
|
| Fund: This work was supported by the National Natural Science Foundation of China (30971773), the Natural Science Foundation of Hebei Province, China (C2011204031) and the Key Laboratory of Crop Growth Regulation of Hebei Province, China. |
|
Corresponding Authors:
Correspondence XIAO Kai, Tel: +86-312-7528115, Fax: +86-312-7528400, E-mail: xiaokai@hebau.edu.cn; LI Xiao-juan, Tel: +86-312-7528245, Fax: +86-312-7528200, E-mail: lxjlixiaojuan@yahoo.com.cn
E-mail: xiaokai@hebau.edu.cn
|
Cite this article:
SUN Zhao-hua, DING Chang-huan, LI Xiao-juan , XIAO Kai.
2012.
Molecular Characterization and Expression Analysis of TaZFP15, a C2H2- Type Zinc Finger Transcription Factor Gene in Wheat (Triticum aestivum L.). Journal of Integrative Agriculture, 12(1): 31-42.
|
| [1]Bari R, Pant B D, Stitt M, Scheible W R. 2006. PHO2, microRNA399, and PHR1 define a phosphate-signaling pathway in plants. Plant Physiology, 141, 988-999. [2]Bewley J D. 1997. Seed germination and dormancy. The Plant Cell, 9, 1055-1066. [3]Chiou T J, Aung K, Lin S I, Wu C C, Chiang S F, Su C L. 2006. Regulation of phosphate homeostasis by microRNA in Arabidopsis. The Plant Cell, 18, 412-421. [4]Christmann A, Moes D, Himmelbach A, Yang Y, Tang Y, Grill E. 2006. Integration of abscisic acid signalling into plant responses. Plant Biology, 8, 314-325. [5]Cutler S, McCourt P. 2005. Dude, where’s my phenotype? Dealing with redundancy in signaling networks. Plant Physiology, 138, 558-559. [6]Devaiah B N, Karthikeyan A S, Raghothama K G. 2007a. WRKY75 transcription factor is a modulator of phosphate acquisition and root development in Arabidopsis. Plant Physiology, 143, 1789-1801. [7]Devaiah B N, Nagarajan V K, Raghothama K G. 2007b. Phosphate homeostasis and root development in Arabidopsis are synchronized by the zinc finger transcription factor ZAT6. Plant Physiology, 145, 147-159. [8]Englbrecht C C, Schoof H, Böhm S. 2004. Conservation, diversification and expansion of C2H2 zinc finger proteins in the Arabidopsis thaliana genome. BMC Genomics, 5, 39. Finkelstein R, Reeves W, Ariizumi T, Steber C. 2008. Molecular aspects of seed dormancy. Annual Review of Plant Biology, 59, 387-415. [9]Finkelstein R R, Gampala S S, Rock C D. 2002. Abscisic acid signaling in seeds and seedlings. The Plant Cell, 14, S15-S45. [10]González E, Solano R, Rubio V, Leyva A, Paz-Ares J. 2005. Phosphate transporter traffic facilitator 1 is a plantspecific SEC12-related protein that enables the endoplasmic reticulum exit of a high-affinity phosphate transporter in Arabidopsis. The Plant Cell, 17, 3500-3512. [11]González-Guzmán M, Apostolova N, Bellés J M, Barrero J M, Piqueras P, Ponce M R, Micol J L, Serrano R, Rodríguez P L. 2002. The short-chain alcohol dehydrogenase ABA2 catalyzes the conversion of xanthoxin to abscisic aldehyde. The Plant Cell, 14, 1833-1846. [12]Guo L, Zhao Y, Zhang S, Zhang H, Xiao K. 2009. Improvement of organic phosphate acquisition in transgenic tobacco plants by overexpression of a soybean phytase gene Sphy1. Frontiers of Agriculture in China, 3, 259-265. [13]Huang J, Yang X, Wang M M, Tang H J, Ding L Y, Shen Y, Zhang H S. 2007. A novel rice C2H2-type zinc finger protein lacking DLN-box/EAR-motif plays a role in salt tolerance. Biochimica et Biophysica Acta, 1769, 220-227. [14]Hugouvieux V, Kwak J M, Schroeder J I. 2001. An mRNA cap binding protein, ABH1, modulates early abscisic acid signal transduction in Arabidopsis. Cell, 106, 477-487. [15]Iida A, Kazuoka T, Torikai S, Kikuchi H, Oeda K. 2000. A zinc finger protein RHL41 mediates the light acclimation response in Arabidopsis. The Plant Journal, 24, 191-203. [16]Iwasaki T, Kiyosue T, Yamaguchi-Shinozaki K, Shinozaki K. 1997. The dehydration-inducible rd17 (cor47) gene and its promoter region in Arabidopsis thaliana. Plant Physiology, 115, 1287-1298. [17]Long S X, Lu W J, Gu J T, Guo C J, Xiao K. 2009. Construction of a cDNA subtractive library enriched the response genes of deficient-Pi stress and functional identification of some ESTs in the library. Acta Agriculturae Boreali-Sinica, 24, 12-16. (in Chinese) [18]Misson J, Raghothama K G, Jain A, Jouhet J, Block M A, Bligny R, Ortet P, Creff A, Somerville S, Rolland N. 2005. A genome-wide transcriptional analysis using Arabidopsis thaliana Affymetrix gene chips determined plant responses to phosphate deprivation. Proceedings of the National Academy of Sciences of the USA, 102, 11934-11939. [19]Mittler R, Kim Y, Song L, Coutu J, Coutu A, Ciftci-Yilmaz S, Lee H, Stevenson B, Zhu J J. 2006. Gain-and loss-offunction mutations in Zat10 enhance the tolerance of plants to abiotic stress. FEBS Letter, 580, 6537-6542. [20]Miura K, Rus A, Sharkhuu A, Yokoi S, Karthikeyan A S, Raghothama K G, Baek D, Koo Y D, Jin J B, Bressan R A. 2005. The Arabidopsis SUMO E3 ligase SIZ1 controls phosphate deficiency responses. Proceedings of the National Academy of Sciences of the USA, 102, 7760-7765. [21]Nakashima K, Yamaguchi-Shinozaki Y. 2006. Regulations involved in osmotic stress-responsive and stressresponsive gene expression in plants. Physiologia Plantarum, 126, 62-71. [22]Nambara E, Marion-Poll A. 2005. Abscisic acid biosynthesis and catabolism. Annual Review of Plant Biology, 56, 165-185. [23]Nemhauser J L, Hong F, Chory J. 2006. Different plant hormones regulate similar processes through largely nonoverlapping transcriptional responses. Cell, 126, 467-475. [24]Pei Z M, Kuchitsu K. 2005. Early ABA signaling events in guard cells. Journal of Plant Growth Regulation, 24, 296-307. [25]Rubio V, Linhares F, Solano R, Martín A C, Iglesias J, Leyva A, Paz-Ares J. 2001. A conserved MYB transcription factor involved in phosphate starvation signalling both in vascular plants and in unicellular algae. Genes & Development, 15, 2122-2133. [26]Sakamoto H, Araki T, Meshi T, Iwabuchi M. 2000. Expression of a subset of the Arabidopsis Cys(2)/His (2)-type zinc-finger protein gene family under water stress. Gene, 248, 23-32. [27]Sakamoto H, Maruyama K, Sakuma Y, Meshi T, Iwabuchi M, Shinozaki K,Yamaguchi-Shinozaki K. 2004. Arabidopsis Cys2/His2-type zinc finger proteins function as transcription repressors under drought, cold and high-salinity stress conditions. Plant Physiology, 136, 2734-2746. [28]Shinozaki K, Yamaguchi-Shinozaki K. 2000. Molecular responses to dehydration and low temperature: Differences and cross-talk between two stress signaling pathways. Current Opinion in Plant Biology, 3, 217-223. [29]Shinozaki K, Yamaguchi-Shinozaki K. 2007. Gene networks involved in drought stress response and tolerance. Journal of Experimental Botany, 58, 221-227. [30]Skriver K, Mundy J. 1990. Gene expression in response to abscisic acid and osmotic stress. The Plant Cell, 2, 503-512. [31]Vogel J T, Zarka D G, van Buskirk H A, Fowler S G, Thomashow M F. 2005. Roles of the CBF2 and ZAT12 transcription factors in configuring the low temperature transcriptome of Arabidopsis. The Plant Journal, 41, 195-211. [32]Wang H, Datla R, Georges F, Loewen M, Cuter A J. 1995. Promoters from Kin1 and cor 6.6, two homologous Arabidopsis thaliana genes: Transcriptional regulation and gene expression induced by low temperature, ABA osmoticum and dehydration. Plant Molecular Biology, 28, 605-617. [33]Wasaki J, Yonetani R, Kuroda S, Shinano T, Yazaki J, Fujii F, Shimbo K, Yamamoto K, Sakata K, Sasaki T. 2003. Transcriptomic analysis of metabolic changes by phosphorus stress in rice plant roots. Plant Cell and Environment, 26, 1515-1523. [34]Wu P, Ma L, Hou X, Wang M, Wu Y, Liu F, Deng X W. 2003. Phosphate starvation triggers distinct alterations of genome expression in Arabidopsis roots and leaves. Plant Physiology, 132, 1260-1271. [35]Xu D Q, Huang J, Guo S Q, Yang X, Bao Y M, Tang H J, Zhang H S. 2008. Overexpression of a TFIIIA-type zinc finger protein gene ZFP252 enhances drought and salt tolerance in rice (Oryza sativa L.). FEBS Letter, 582, 1037-1043. [36]Yamaguchi-Shinozaki K, Shinozaki K. 2006. Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses. Annual Review of Plant Biology, 57, 781-803. [37]Yi K, Wu Z, Zhou J, Du L, Guo L, Wu Y, Wu P. 2005. OsPTF1, a novel transcription factor involved in tolerance to phosphate starvation in rice. Plant Physiology, 138, 2087-2096. [38]Zhang H N, Guo C J, Li C D, Xiao K. 2008. Cloning, characterization and expression analysis of two superoxide dismutase (SOD) genes in wheat (Triticum aestivum L.). Frontiers of Agriculture in China, 2, 141-149. [39]Zhu J K. 2002. Salt and drought stress signal transduction in plants. Annual Review of Plant Biology, 53, 247-273. |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
