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Journal of Integrative Agriculture  2021, Vol. 20 Issue (10): 2666-2679    DOI: 10.1016/S2095-3119(20)63587-0
Special Issue: 园艺-分子生物合辑Horticulture — Genetics · Breeding
Horticulture Advanced Online Publication | Current Issue | Archive | Adv Search |
Genome-wide analysis of the SCPL gene family in grape (Vitis vinifera L.)
WANG Xi-cheng, WU Wei-min, ZHOU Bei-bei, WANG Zhuang-wei, QIAN Ya-ming, WANG Bo, YAN Li-chun

Institute of Pomology, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, P.R.China
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Serine carboxypeptidase-like (SCPL) proteins are a group of acyltransferase enzymes that have important roles in plant growth, development, and stress responses.  Although SCPL proteins have been studied in many plants, the biological functions of SCPL genes in grape are still unknown.  In this study, 59 putative SCPL proteins were identified from the grape genome.  A bioinformatics analysis, including chromosomal locations, exon/intron structures, phylogeny, cis-elements, and conserved motifs, was performed for the gene family.  The phylogenetic analysis revealed that VvSCPL proteins could be classified into three groups, with the gene motifs in each group showing high similarity levels.  The number of exons in the VvSCPL genes ranged from 1 to 19, suggesting significant variations among grape SCPL genes.  The expression of the VvSCPL genes, as assessed by RNA sequencing (RNA-seq) and quantitative real-time PCR, showed that most VvSCPL genes responded to drought- and waterlogging-stress treatments, which indicated their roles in abiotic stress responses.  The results provide useful information for further study of SCPL genes in grape.
Keywords:  serine carboxypeptidase-like (SCPL) protein        grape        phylogenetic relationships        abiotic stress        gene expression  
Received: 10 April 2020   Accepted:
Fund: This research was supported by the National Key Research and Development Program of China (2018YFD0201300), the Natural Science Funds of Jiangsu Province, China (BK20160587) and the China Agriculture Research System of MOF and MARA (CARS-29-11).
Corresponding Authors:  Correspondence WU Wei-min, Tel/Fax: +86-25-84392902, E-mail:   
About author:  WANG Xi-cheng, E-mail:;

Cite this article: 

WANG Xi-cheng, WU Wei-min, ZHOU Bei-bei, WANG Zhuang-wei, QIAN Ya-ming, WANG Bo, YAN Li-chun. 2021. Genome-wide analysis of the SCPL gene family in grape (Vitis vinifera L.). Journal of Integrative Agriculture, 20(10): 2666-2679.

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