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Journal of Integrative Agriculture  2021, Vol. 20 Issue (1): 46-54    DOI: 10.1016/S2095-3119(19)62830-3
Special Issue: 麦类遗传育种合辑Triticeae Crops Genetics · Breeding · Germplasm Resources
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TaSnRK2.4 is a vital regulator in control of thousand-kernel weight and response to abiotic stress in wheat
MIAO Li-li1, 2, LI Yu-ying3, ZHANG Hong-juan3, ZHANG Hong-ji2, LIU Xiu-lin4, WANG Jing-yi3, CHANG Xiao-ping3, MAO Xin-guo3, JING Rui-lian
1 Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme, Harbin 150086, P.R.China
2 Institute of Crop Resources, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, P.R.China
3 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
4 Institute of Soybean, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, P.R.China
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Sucrose non-fermenting 1-related protein kinase 2 (SnRK2) is a plant-specific serine/threonine kinase involved in response to adverse environmental stimuli.  Previous studies showed that TaSnRK2.4 was involved in response to abiotic stresses and conferred enhanced tolerance to multiple stresses in Arabidopsis.  Further experiments were performed to decipher the underlying mechanisms and discover new functions.  The genomic sequences of TaSnRK2.4s locating on chromosome 3A, 3B and 3D were obtained.  Sequencing identified one and 13 variations of TaSnRK2.4-3A and TaSnRK2.4-3B, respectively, but no variation was detected in TaSnRK2.4-3D.  The markers 2.4AM1, 2.4BM1 and 2.4BM2 were developed based on three variations.  Association analysis showed that both TaSnRK2.4-3A and TaSnRK2.4-3B were significantly associated with thousand-kernel weight (TKW), and that SNP3A-T and SNP3B-C were favorable alleles for higher TKW.  Yeast two-hybrid and split luciferase assays showed that TaSnRK2.4 physically interacted with abiotic stress responsive protein TaLTP3, suggesting that TaSnRK2.4 enhanced abiotic stress tolerance by activating TaLTP3.  Our studies suggested that TaSnRK2.4 have potential in improving TKW and response to abiotic stress.
Keywords:  TaSnRK2.4        association analysis        TKW        abiotic stress  
Received: 21 August 2019   Accepted:
Fund: This work was supported by the National Key Research and Development Program of China (2017YFD0300202), the National Natural Science Foundation of China (31571660), the Heilongjiang Postdoctoral Financial Assistance, China (LBH-Z17200), and the Research Project of Heilongjiang Academy of Agricultural Sciences, China (2018YYYF018).
Corresponding Authors:  Correspondence MAO Xin-guo, Tel: +86-10-82105829, E-mail:; JING Rui-lian, Tel: +86-10-82105829, E-mail:   

Cite this article: 

MIAO Li-li, LI Yu-ying, ZHANG Hong-juan, ZHANG Hong-ji, LIU Xiu-lin, WANG Jing-yi, CHANG Xiao-ping, MAO Xin-guo, JING Rui-lian. 2021. TaSnRK2.4 is a vital regulator in control of thousand-kernel weight and response to abiotic stress in wheat. Journal of Integrative Agriculture, 20(1): 46-54.

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