|
Special Issue:
麦类遗传育种合辑Triticeae Crops Genetics · Breeding · Germplasm Resources
|
|
|
|
| dCAPS markers developed for nitrate transporter genes TaNRT2L12s associating with 1 000-grain weight in wheat |
HUANG Jun-fang1, 2, LI Long2, MAO Xin-guo2, WANG Jing-yi2, LIU Hui-min1, LI Chao-nan2, JING Rui-lian2 |
1 College of Bioengineering, Shanxi University, Taiyuan 030006, P.R.China
2 National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China |
|
|
|
|
Abstract Nitrate transporters (NRTs) are regulators of nitrate assimilation and transport. The genome sequences of TaNRT2L12-A, -B and -D were cloned from wheat (Triticum aestivum L.), and polymorphisms were analyzed by sequencing. TaNRT2L12-D in a germplasm population was highly conserved. However, 38 single nucleotide polymorphisms (SNPs) in TaNRT2L12-A coding region and 11 SNPs in TaNRT2L12-B coding region were detected. Two derived cleaved amplified polymorphic sequences (dCAPS) markers A-CSNP1 and A-CSNP2 were developed for TaNRT2L12-A based on SNP-351 and SNP-729, and three haplotypes were identified in the germplasm population. B-CSNP1 and B-CSNP2 were developed for TaNRT2L12-B based on SNP-237 and SNP-1 227, and three haplotypes were detected in the germplasm population. Association analyses between the markers and agronomic traits in 30 environments and phenotypic comparisons revealed that A-CSNP2-A is a superior allele of shorter plant height (PH), length of penultimate internode (LPI) and peduncle length (PL), B-CSNP2-G is a superior allele of higher grain number per spike (GNS). Hap-6B-1 containing both superior alleles B-CSNP1-C and B-CSNP2-A is a superior haplotype of 1 000-grain weight (TGW). Expression analysis showed that TaNRT2L12-B is mainly expressed in the root base and regulated by nitrate. Therefore, TaNRT2L12 may be involved in nitrate transport and signaling to regulate TGW in wheat. The superior alleles and dCAPS markers of TaNRT2L12-A/B are beneficial to genetic improvement and germplasm enhancement with molecular markers-assisted selection.
|
|
Received: 18 February 2019
Accepted:
|
| Fund: This work was supported by the National Key Research and Development Program of China (2017YFD0300202) and the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences (ASTIP). |
|
Corresponding Authors:
Correspondence LI Chao-nan, Tel: +86-10-82105829, E-mail: lichaonan@caas.cn; JING Rui-lian, Tel: +86-10-82105829, E-mail: jingruilian@caas.cn
|
Cite this article:
HUANG Jun-fang, LI Long, MAO Xin-guo, WANG Jing-yi, LIU Hui-min, LI Chao-nan, JING Rui-lian.
2020.
dCAPS markers developed for nitrate transporter genes TaNRT2L12s associating with 1 000-grain weight in wheat. Journal of Integrative Agriculture, 19(6): 1543-1553.
|
Agrama H A, Eizenga G C, Yan W. 2007. Association mapping of yield and its components in rice cultivars. Molecular Breeding, 19, 341–356.
Castro M I, Loef I, Bartetzko L, Searle I, Coupland G, Stitt M, Osuna D. 2011. Nitrate regulates floral induction in Arabidopsis, acting independently of light, gibberellin and autonomous pathways. Planta, 233, 539–552.
Cai C, Zhao X, Zhu Y, Tong Y, Li Z. 2007. Regulation of the high-affinity nitrate transport system in wheat roots by exogenous abscisic acid and glutamine. Journal of Integrative Plant Biology, 49, 1719–1725.
Cao X, Zhong C, Zhu L, Zhang J, Hussain S, Wu L, Jin Q. 2017. Glycine increases cold tolerance in rice via the regulation of N uptake, physiological characteristics, and photosynthesis. Plant Physiology and Biochemistry, 112, 251–260.
Crawford N M, Forde B G. 2002. Molecular and developmental biology of inorganic nitrogen nutrition. Arabidopsis Book, 1, e0011.
Dechorgnat J, Patrit O, Krapp A, Fagard M, Daniel-Vedele F. 2012. Characterization of the Nrt2.6 gene in Arabidopsis thaliana: A link with plant response to biotic and abiotic stress. PLoS ONE, 7, e42491.
Evanno G, Regnaut S, Goudet J. 2005. Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Molecular Ecology, 14, 2611–2620.
Evenson R E, Gollin D. 2003. Assessing the impact of the green revolution, 1960 to 2000. Science, 300, 758–762.
Feng H, Li B, Zhi Y, Chen J, Li R, Xia X, Xu G, Fan X. 2017. Overexpression of the nitrate transporter, OsNRT2.3b, improves rice phosphorus uptake and translocation. Plant Cell Reports, 36, 1287–1296.
Feng H, Yan M, Fan X, Li B, Shen Q, Miller A J, and Xu G. 2011. Spatial expression and regulation of rice high-affinity nitrate transporters by nitrogen and carbon status. Journal of Experimental Botany, 62, 2319–2332.
Fernandez E, Galvan A. 2008. Nitrate assimilation in Chlamydomonas. Eukaryot Cell, 7, 555–559.
Galvan A, Fernandez E. 2001. Eukaryotic nitrate and nitrite transporters. Cellular and Molecular Life Sciences, 58, 225–233.
Glass A D, Shaff J E, Kochian L V. 1992. Studies of the uptake of nitrate in barley: IV. Electrophysiology. Plant Physiology, 99, 456–463.
Gregersen P L, Holm P B, Krupinska K. 2008. Leaf senescence and nutrient remobilisation in barley and wheat. Plant Biology (Stuttg), 10(Suppl. 1), 37–49.
Gu C, Song A, Zhang X, Wang H, Li T, Chen Y, Jiang J, Chen F, Chen S. 2016. Cloning of chrysanthemum high-affinity nitrate transporter family (CmNRT2) and characterization of CmNRT2.1. Scientific Reports, 6, 23462.
Gupta P K, Rustgi S, Kulwal P L. 2005. Linkage disequilibrium and association studies in higher plants: Present status and future prospects. Plant Molecular Biology, 57, 461–485.
He X, Qu B, Li W, Zhao X, Teng W, Ma W, Ren Y, Li B, Li Z, Tong Y. 2015. The nitrate-inducible NAC transcription factor TaNAC2-5A controls nitrate response and increases wheat yield. Plant Physiology, 169, 1991–2005.
Kotur Z, Glass A D. 2015. A 150 kDa plasma membrane complex of AtNRT2.5 and AtNAR2.1 is the major contributor to constitutive high-affinity nitrate influx in Arabidopsis thaliana. Plant Cell and Environment, 38, 1490–1502.
Kotur Z, Mackenzie N, Ramesh S, Tyerman S D, Kaiser B N, Glass A D. 2012. Nitrate transport capacity of the Arabidopsis thaliana NRT2 family members and their interactions with AtNAR2.1. New Phytologist, 194, 724–731.
Li H, Hu B, Chu C. 2017. Nitrogen use efficiency in crops: Lessons from Arabidopsis and rice. Journal of Experimental Botany, 68, 2477–2488.
Little D Y, Rao H, Oliva S, Daniel-Vedele F, Krapp A, Malamy J E. 2005. The putative high-affinity nitrate transporter NRT2.1 represses lateral root initiation in response to nutritional cues. Proceedings of the National Academy of Sciences of the United States of America, 102, 13693–13698.
Luo B, Chen J, Zhu L, Liu S, Li B, Lu H, Ye G, Xu G, Fan X. 2018. Overexpression of a high-affinity nitrate transporter osnrt2.1 increases yield and manganese accumulation in rice under alternating wet and dry condition. Frontiers in Plant Science, 9, 1192.
Lupini A, Mercati F, Araniti F, Miller A J, Sunseri F, Abenavoli M R. 2016. NAR2.1/NRT2.1 functional interaction with NO3(–) and H(+) fluxes in high-affinity nitrate transport in maize root regions. Plant Physiology and Biochemistry, 102, 107–114.
Miller A J, Fan X, Orsel M, Smith S J, Wells D M. 2007. Nitrate transport and signalling. Journal of Experimental Botany, 58, 2297–2306.
Ray D K, Ramankutty N, Mueller N D, West P C, Foley J A. 2012. Recent patterns of crop yield growth and stagnation. Nature Communications, 3, 1293.
Sadras V O, Richards R A. 2014. Improvement of crop yield in dry environments: Benchmarks, levels of organisation and the role of nitrogen. Journal of Experimental Botany, 65, 1981–1995.
Simmonds J, Scott P, Leverington-Waite M, Turner A S, Brinton J, Korzun V, Snape J, Uauy C. 2014. Identification and independent validation of a stable yield and thousand grain weight QTL on chromosome 6A of hexaploid wheat (Triticum aestivum L.). BMC Plant Biology, 14, 191.
Taulemesse F, Le Gouis J, Gouache D, Gibon Y, Allard V. 2015. Post-flowering nitrate uptake in wheat is controlled by N status at flowering, with a putative major role of root nitrate transporter NRT2.1. PLoS ONE, 10, e0120291.
Vidmar J J, Zhuo D, Siddiqi M Y, Glass A D. 2000. Isolation and characterization of HvNRT2.3 and HvNRT2.4, cDNAs encoding high-affinity nitrate transporters from roots of barley. Plant Physiology, 122, 783–792.
Wei J, Zheng Y, Feng H, Qu H, Fan X, Yamaji N, Ma J F, Xu G. 2018. OsNRT2.4 encodes a dual-affinity nitrate transporter and functions in nitrate-regulated root growth and nitrate distribution in rice. Journal of Experimental Botany, 69, 1095–1107.
Williams L, Miller A. 2001. Transporters responsible for the uptake and partitioning of nitrogenous solutes. Annual Review of Plant Physiology and Plant Molecular Biology, 52, 659–688.
Yan M, Fan X, Feng H, Miller A J, Shen Q, Xu G. 2011. Rice OsNAR2.1 interacts with OsNRT2.1, OsNRT2.2 and OsNRT2.3a nitrate transporters to provide uptake over high and low concentration ranges. Plant Cell Environment, 34, 1360–1372.
Zhang H, Mao X, Zhang J, Chang X, Jing R. 2013. Single-nucleotide polymorphisms and association analysis of drought-resistance gene TaSnRK2.8 in common wheat. Plant Physiology and Biochemistry, 70, 174–181.
Zhao X Q, Li Y J, Liu J Z, Li B, Liu Q Y, Tong Y P, Li J Y, Li Z S. 2004. Isolation and expression analysis of a high-affinity nitrate transporter TaNRT2.3 from roots of wheat. Acta Botanica Sinica, 46, 347–354.
|
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
