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Journal of Integrative Agriculture  2022, Vol. 21 Issue (12): 3540-3555    DOI: 10.1016/j.jia.2022.08.104
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Genome-wide identification and characterization of the JAZ gene family and its expression patterns under various abiotic stresses in Sorghum bicolor

DU Qiao-li1*, FANG Yuan-peng1*, JIANG Jun-mei2, CHEN Mei-qing1, LI Xiang-yang2, XIE Xin1

1 Key Laboratory of Agricultural Microbiology, College of Agriculture, Guizhou University, Guiyang 550025, P.R.China 
2 State Key Laboratory Breeding Base of Green Pesticides and Agricultural Bioengineering/Key Laboratory of Green Pesticides and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, P.R.China
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JAZThe jasmonate ZIM domain)蛋白属于TIFY(TIF[F/Y]XG)家族,它是由植物特异性蛋白组成,在植物生长发育和防御反应中具有十分重要的作用。然而,对于高粱JAZ家族基因植物响应非生物胁迫的机制尚不清楚。本研究采用隐马尔可夫模型,在高粱中共鉴定到17JAZ基因。此外,采用实时荧光定量PCR (RT-qPCR) 对高粱JAZ基因在非生物胁迫下的表达模式进行分析。系统发育分析表明,高粱JAZ蛋白主要分为9个亚家族,启动子区包含多种类型的启动子顺式作用元件,表明JAZ蛋白在植物的胁迫响应中起作用。RT-qPCR结果显示SbJAZ家族基因的表达具有组织特异性,在冷、热、聚乙二醇、茉莉酸、脱落酸和赤霉素处理下,SbJAZ基因的表达出现明显差异,表明SbJAZ基因参与植物对不同胁迫的响应。此外,在大肠杆菌中表达SbJAZ1可促进重组细胞在非生物胁迫(PEG 6000NaCl 40°C)下的生长。综上所述,本研究结果将有助于更好地了解高粱SbJAZ家族响应非生物胁迫的潜在分子机制。


The jasmonate ZIM domain (JAZ) protein belongs to the TIFY ((TIF[F/Y]XG) domain protein) family, which is composed of several plant-specific proteins that play important roles in plant growth, development, and defense responses.  However, the mechanism of the sorghum JAZ family in response to abiotic stress remains unclear.  In the present study, a total of 17 JAZ genes were identified in sorghum using a Hidden Markov Model search.  In addition, real-time quantification polymerase chain reaction (RT-qPCR) was used to analyze the gene expression patterns under abiotic stress.  Based on phylogenetic tree analysis, the sorghum JAZ proteins were mainly divided into nine subfamilies.  A promoter analysis revealed that the SbJAZ family contains diverse types of promoter cis-acting elements, indicating that JAZ proteins function in multiple pathways upon stress stimulation in plants.  According to RT-qPCR, SbJAZ gene expression is tissue-specific.  Additionally, under cold, hot, polyethylene glycol, jasmonic acid, abscisic acid, and gibberellin treatments, the expression patterns of SbJAZ genes were distinctly different, indicating that the expression of SbJAZ genes may be coordinated with different stresses.  Furthermore, the overexpression of SbJAZ1 in Escherichia coli was found to promote the growth of recombinant cells under abiotic stresses, such as PEG 6000, NaCl, and 40°C treatments.  Altogether, our findings help us to better understand the potential molecular mechanisms of the SbJAZ family in sorghum in response to abiotic stresses.

Keywords:  Sorghum bicolor       gene family identification        JAZ family        abiotic stress        expression pattern  
Received: 09 August 2021   Accepted: 23 November 2021
Fund: This research was funded by the National Natural Science Foundation of China (32060614 and 32272514), the Guizhou Provincial Science and Technology Project, China ([2022]091), and the China Postdoctoral Science Foundation (2022MD713740).

About author:  DU Qiao-li, E-mail:; FANG Yuan-peng, E-mail:; Correspondence XIE Xin, Mobile: +86-17585108412, E-mail: * These authors contributed equally to this study.

Cite this article: 

DU Qiao-li, FANG Yuan-peng, JIANG Jun-mei, CHEN Mei-qing, LI Xiang-yang, XIE Xin. 2022. Genome-wide identification and characterization of the JAZ gene family and its expression patterns under various abiotic stresses in Sorghum bicolor. Journal of Integrative Agriculture, 21(12): 3540-3555.

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