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Journal of Integrative Agriculture  2020, Vol. 19 Issue (5): 1170-1185    DOI: 10.1016/S2095-3119(19)62776-0
Special Issue: Triticeae Crops Genetics · Breeding · Germplasm Resources
Crop Science Advanced Online Publication | Current Issue | Archive | Adv Search |
Bioinformatic identification and analyses of the non-specific lipid transfer proteins in wheat
FANG Zheng-wu1, HE Yi-qin1, LIU Yi-ke2, JIANG Wen-qiang1, SONG Jing-han1, WANG Shu-ping1, MA Dong-fang1, 3, YIN Jun-liang1  
1 Hubei Collaborative Innovation Center for Grain Industry/Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education/Hubei Engineering Research Center for Pest Forewarning and Management/College of Agriculture, Yangtze University, Jingzhou 434025, P.R.China
2 Institute of Food Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064, P.R.China
3 Institute of Plant Protection, Sichuan Academy of Agricultural Sciences/Key Laboratory of Integrated Pest Management of Crop in Southwest China, Ministry of Agriculture and Rural Affairs, Chengdu 610066, P.R.China
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Non-specific lipid transfer proteins (nsLTPs/LTPs) that can transport various phospholipids across the membrane in vitro are widespread in the plant kingdom, and they play important roles in many biological processes that are closely related to plant growth and development.  Recently, nsLTPs have been shown to respond to different forms of abiotic stresses.  Despite the vital roles of nsLTPs in many plants, little is known about the nsLTPs in wheat.  In this study, 330 nsLTP proteins were identified in wheat and they clustered into five types (1, 2, c, d, and g) by phylogenetic analysis with the nsLTPs from maize, Arabidopsis, and rice.  The wheat nsLTPs of type d included three subtypes (d1, d2, and d3) and type g included seven subtypes (g1–g7).  Genetic structure and motif pattern analyses showed that members of each type had similar structural composition.  Moreover, GPI-anchors were found to exist in non-g type members from wheat for the first time.  Chromosome mapping revealed that all five types were unevenly and unequally distributed on 21 chromosomes.  Furthermore, gene duplication events contributed to the proliferation of the nsLTP genes.  Large-scale data mining of RNA-seq data covering multiple growth stages and numerous stress treatments showed that the transcript levels of some of the nsLTP genes could be strongly induced by abiotic stresses, including drought and salinity, indicating their potential roles in mediating the responses of the wheat plants to these abiotic stress conditions.  These findings provide comprehensive insights into the nsLTP family members in wheat, and offer candidate nsLTP genes for further studies on their roles in stress resistance and potential for improving wheat breeding programs.
Keywords:  nsLTP        abiotic stress        wheat genome        bioinformatics        gene family  
Received: 29 January 2019   Accepted: 25 March 2020
Fund: This work was supported by the National Transgenic Key Project of the Ministry of Agriculture of China (2018ZX0800909B), the Major Program of Technological Innovation of Hubei Province, China (2018ABA085), the Open Project Program of Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education, China (KF201802), and the Southwest Agricultural Crop Pest Management Key Laboratory Open Fund of Ministry of Agriculture, China (2018-XNZD-01).
Corresponding Authors:  Correspondence MA Dong-fang, Tel: +86-716-8066302, E-mail:; YIN Jun-liang, Tel: +86-716-8066302, E-mail:    

Cite this article: 

FANG Zheng-wu, HE Yi-qin, LIU Yi-ke, JIANG Wen-qiang, SONG Jing-han, WANG Shu-ping, MA Dong-fang, YIN Jun-liang. 2020. Bioinformatic identification and analyses of the non-specific lipid transfer proteins in wheat. Journal of Integrative Agriculture, 19(5): 1170-1185.

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