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| Detection and Characterization of β-Lactam Resistance in Haemophilus parasuis Strains from Pigs in South China |
| GUO Li-li, ZHANG Jian-min, XU Cheng-gang, REN Tao, ZHANG Bin, CHEN Ji-dang , LIAO Ming |
| 1.Key Laboratory of Animal Disease Control and Prevention, Ministry of Agriculture/Key Laboratory of Zoonoses Prevention and Control of Guangdong, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, P.R.China |
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摘要 To characterize the β-lactam resistance in veterinary clinical isolates of Haemophilus parasuis, 115 isolates were examined for the β-lactam resistance, the possession of β-lactamase, and the presence of β-lactamase genes. The genetic relationship among isolates was evaluated by pulsed-field gel electrophoresis (PFGE). Overall, the commonly detected resistance phenotypes were resistant to ampicillin (26.09%), penicillin (22.61%), amoxicillin (21.74%), cefazolin (14.78%), cefaclor (12.17%), and cefotaxime (6.96%). These strains showed high minimal inhibitory concentration (MICs) to oxacillin. 20.87% strains produced β-lactamase, and 4.35% strains showed extended-spectrum b-lactamase (ESBL) phenotype. Moreover, 19 strains harboured bla genes including TEM-1 (n=5), TEM-116 (n=10), and ROB-1 (n=5). Significantly, one strain possessed both TEM-1 and ROB-1, and displayed resistance to cefotaxime (MIC=8 mg L-1). The epidemiological analysis of PFGE revealed high genetic diversity among bla-positive isolates. This work shows that TEM- and ROB-type β-lactamases are prevalent in H. parasuis isolates in China.
Abstract To characterize the β-lactam resistance in veterinary clinical isolates of Haemophilus parasuis, 115 isolates were examined for the β-lactam resistance, the possession of β-lactamase, and the presence of β-lactamase genes. The genetic relationship among isolates was evaluated by pulsed-field gel electrophoresis (PFGE). Overall, the commonly detected resistance phenotypes were resistant to ampicillin (26.09%), penicillin (22.61%), amoxicillin (21.74%), cefazolin (14.78%), cefaclor (12.17%), and cefotaxime (6.96%). These strains showed high minimal inhibitory concentration (MICs) to oxacillin. 20.87% strains produced β-lactamase, and 4.35% strains showed extended-spectrum b-lactamase (ESBL) phenotype. Moreover, 19 strains harboured bla genes including TEM-1 (n=5), TEM-116 (n=10), and ROB-1 (n=5). Significantly, one strain possessed both TEM-1 and ROB-1, and displayed resistance to cefotaxime (MIC=8 mg L-1). The epidemiological analysis of PFGE revealed high genetic diversity among bla-positive isolates. This work shows that TEM- and ROB-type β-lactamases are prevalent in H. parasuis isolates in China.
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Received: 03 December 2010
Accepted:
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| Fund: This work was supported by the Program for New Century Excellent Talents in University, China (NCET-06-0752), and the Guangdong Technology Planning Committee, China (2006B0152 and 2009A0201006). |
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Corresponding Authors:
Correspondence LIAO Ming, Tel: +86-20-85280240, Fax: +86-20-85285282, E-mail: mliao@scau.edu.cn
E-mail: mliao@scau.edu.cn
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| About author: GUO Li-li, E-mail: gll2004203746@126.com |
Cite this article:
GUO Li-li, ZHANG Jian-min, XU Cheng-gang, REN Tao, ZHANG Bin, CHEN Ji-dang , LIAO Ming.
2012.
Detection and Characterization of β-Lactam Resistance in Haemophilus parasuis Strains from Pigs in South China. Journal of Integrative Agriculture, 12(1): 116-121.
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