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Development and characterization of wheat–Aegilops kotschyi 1Uk(1A) substitution line with positive dough quality parameters
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| JIANG Yun1, 2, 3*, WANG De-li1, 3*, HAO Ming1, 3, ZHANG Jie2, LIU Deng-cai1, 3# |
1 State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, P.R.China
2 Biotechnology and Nuclear Technology Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610061, P.R.China
3 Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China
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摘要
从野生近缘种中发掘新的高分子量谷蛋白亚基（HMW-GS）是改良小麦加工品质的有效途径。本研究旨在鉴定一份小麦-粘果山羊草渐渗系N124的染色体组成，并评价其对小麦品质相关性状的影响。荧光原位杂交（FISH）核型分析表明，N124是一个1Uk(1A)二体代换系。十二烷基磺酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和反相高效液相色谱（RP-HPLC）发现N124表达了两个源自粘果山羊草的HMW-GS。PacBio RNA测序和系统发育分析证实了这两个HMW-GS分别为Ukx和Uky。与其小麦亲本相比，N124除穗粒数较少外，无明显农艺性状缺陷，同时多项主要品质指标均有提高，表明N124可作为小麦品质改良的桥梁材料加以利用。
Abstract
Exploring novel high molecular weight glutenin subunits (HMW-GSs) from wild related species is a strategy to improve wheat processing quality. The objective of the present investigation was to identify the chromosomes of the wheat-alien introgression line N124, derived from the hybridization between Triticum aestivum with Aegilops kotschyi, and characterize the effects on quality-related traits. Fluorescence in situ hybridization karyotypes showed that N124 is a disomic 1Uk(1A) substitution line. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and reversed-phase high-performance liquid chromatography verified N124 expressed two HMW-GSs of the Ae. kotschyi parent. PacBio RNA sequencing and phylogenetic analysis confirmed that the two HMW-GSs were Ukx and Uky. Compared to the wheat parent, the substitution line had no obvious agronomic defects except fewer grains per spike but improved several major quality parameters. It can be served as a donor or bridge material for wheat quality improvement.
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Received: 25 January 2022
Accepted: 16 March 2022
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This work was supported by the National Natural Science Foundation of China (91935303), the Sichuan Province Science and Technology Department Crops Breeding Project, China (2021YFYZ0002), the Crop Molecular Breeding Platform of Sichuan Province, China (2021YFYZ0027), the Foundation for Youth of Sichuan Academy of Agricultural Sciences and the Sichuan Provincial Agricultural Department Innovative Research Team, China (wheat-10).
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| About author: JIANG Yun, E-mail: 85197544@qq.com; #Correspondence LIU Deng-cai, Tel/Fax: +86-28-86290004, E-mail: dcliu7@ sicau.edu.cn
* These authors contributed equally to this study. |
Cite this article:
JIANG Yun, WANG De-li, HAO Ming, ZHANG Jie, LIU Deng-cai.
2023.
Development and characterization of wheat–Aegilops kotschyi 1Uk(1A) substitution line with positive dough quality parameters
. Journal of Integrative Agriculture, 22(4): 999-1008.
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