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Journal of Integrative Agriculture  2016, Vol. 15 Issue (1): 10-17    DOI: 10.1016/S2095-3119(15)61038-3
Crop Genetics · Breeding · Germplasm Resources Advanced Online Publication | Current Issue | Archive | Adv Search |
A genetic evidence of chromosomal fragment from bridge parent existing in substitution lines between two common wheat varieties
 ZHAO Pei, WANG Ke, LIN Zhi-shan, LIU Hui-yun, LI Xin, DU Li-pu, YAN Yue-ming, YE Xing-guo
1、National Key Facility of Crop Gene Resources and Genetic Improvement/Institute of Crop Science, Chinese Academy of
Agricultural Sciences, Beijing 100081, P.R.China
2、Key Laboratory of Genetics and Biotechnology, College of Life Science, Capital Normal University, Beijing 100048, P.R.China
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摘要  Locating of important agronomic genes onto chromosome is helpful for efficient development of new wheat varieties. Wheat chromosome substitution lines between two varieties have been widely used for locating genes because of their distinctive advantages in genetic analysis, compared with the aneuploid genetic materials. Apart from the substituted chromosome, the other chromosomes between the substitution lines and their recipient parent should be identical, which eases the gene locating practice. In this study, a set of chromosome substitution lines with cv. Wichita (WI) as the recipient parent and cv. Cheyenne (CNN) as the donor parent were studied for the composition of high molecular weight glutenin subunits (HMW-GS) as well as a range of agronomic important traits. Results revealed that the substitution lines of WI(CNN5D), WI(CNN6A) and WI(CNN7B) had higher plant heights than the two parents of WI and CNN, and WI(CNN3D) had later maturity than the parents. By sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis, a substitution line WI(CNN5B) was found to contain different HMW-GS patterns from its two parents, in which 1By9 was replaced by 1By8 on chromosome 1BL. Simple sequence repeat (SSR) analysis confirmed that the variation on 1BL in WI(CNN5B) was originated from Chinese Spring (CS). It is concluded that chromosomal fragments from bridge material and donor parent were quite often retained in intracultivaral chromosome substitution lines except the substituting chromosomes.

Abstract  Locating of important agronomic genes onto chromosome is helpful for efficient development of new wheat varieties. Wheat chromosome substitution lines between two varieties have been widely used for locating genes because of their distinctive advantages in genetic analysis, compared with the aneuploid genetic materials. Apart from the substituted chromosome, the other chromosomes between the substitution lines and their recipient parent should be identical, which eases the gene locating practice. In this study, a set of chromosome substitution lines with cv. Wichita (WI) as the recipient parent and cv. Cheyenne (CNN) as the donor parent were studied for the composition of high molecular weight glutenin subunits (HMW-GS) as well as a range of agronomic important traits. Results revealed that the substitution lines of WI(CNN5D), WI(CNN6A) and WI(CNN7B) had higher plant heights than the two parents of WI and CNN, and WI(CNN3D) had later maturity than the parents. By sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis, a substitution line WI(CNN5B) was found to contain different HMW-GS patterns from its two parents, in which 1By9 was replaced by 1By8 on chromosome 1BL. Simple sequence repeat (SSR) analysis confirmed that the variation on 1BL in WI(CNN5B) was originated from Chinese Spring (CS). It is concluded that chromosomal fragments from bridge material and donor parent were quite often retained in intracultivaral chromosome substitution lines except the substituting chromosomes.
Keywords:  wheat       intracultivaral chromosome substitution lines       agronomic traits       high molecular weight glutenin subunits (HMW-GS)       molecular markers  
Received: 04 January 2015   Accepted:
Fund: 

This research was financially supported by grants in part from the National Natural Science Foundation of China (31371621 and 31271703). Authors are grateful to Dr. Li Jiarui at Bayer Cropscience, USA, for critical revision of this manuscript.

Corresponding Authors:  YE Xing-guo, Tel: +86-10-82105173, Fax: +86-10-82109765, E-mail: yexingguo@caas.cn     E-mail:  yexingguo@caas.cn
About author:  * These authors contributed equally to this study.

Cite this article: 

ZHAO Pei, WANG Ke, LIN Zhi-shan, LIU Hui-yun, LI Xin, DU Li-pu, YAN Yue-ming, YE Xing-guo. 2016. A genetic evidence of chromosomal fragment from bridge parent existing in substitution lines between two common wheat varieties. Journal of Integrative Agriculture, 15(1): 10-17.

Bai Z Y, Li C D, Feng L X, Sun H C. 2007. Chromosomallocalization of genes associated with spikelet differentiationand drought tolerance in Chinese Spring (recipient)/synthetic 6x (donor). Scientia Agricultura Sinica, 40,2136-2144 (in Chinese)

Bai Z Y, Li C D, Zhao J F, Wu T Y, Zheng J F, Bi C R. 2001.The effect and preliminary analysis of chromosomal controlon the chlorophyll fluorescence parameters of wheatsubstitution lines between synthetic hexaploid wheat andChinese Spring under drought stress. Scientia AgriculturaSinica, 44, 47-57 (in Chinese)

Bassam B J, Caetano-Anolles G, Gresshoff P M. 1991. Fastand sensitive silver staining of DNA in polyacrylamide gel.Analytical Biochemistry, 196, 80-83

Bazenziger P S, Dweikat I, Gill K, Eskridge K, Berke KT, Campbell B D, Ali M L, Mengistu N, Mahmood A,Auvuchanon A, Yen Y, Rustgi S, Moreno-Sevilia B, Mujeeb-Kazi A, Morris M R. 2011. Understanding grain yield: It is ajourney, not a destination. Czech Journal of Genetics PlantBreeding, 47(Special issue), S77-S84.

Berke T G, Baenziger P S, Morris R. 1992a. Location of wheatquantitative trait loci affecting agronomic performance ofseven traits using reciprocal chromosome substitutions.Crop Science, 32, 621–627.

Berke T G, Baenziger P S, Morris R. 1992b. Locations of wheatquantitative trait loci affecting stability of six traits usingreciprocal chromosome substitutions. Crop Science, 32,628–633.

Campbell B T, Baenziger P S, Gill K S, Eskridge K M, Budak H,Erayman M, Dweikat I, Yen Y. 2003. Identification of QTLsand environmental interactions associated with agronomictraits on chromosome 3A of wheat. Crop Science, 43,1493–1505.

Chen F, Beecher B S, Morris C F. 2010. Physical mapping and anew variant of Puroindoline b-2 genes in wheat Theoreticaland Applied Genetics, 120, 745-751

Dilbirligi M, Erayman M, Campbell B T, Rand hawa H S,Baenziger P S, Dweikat I, Gill K S. 2004. High-densitymapping and comparative analy sis of agronomicallyimportant traits on wheat chro mosome 3A. Genomics, 88,74-87

Farshadfar E, Köszegi B, Tischner T, Sutka J. 1995. Substitutionanalysis of drought tolerance in wheat (Triticum aestivumL.). Plant Breeding, 114, 542-544

Galiba G, Koscy G, Kaur-Sawhney R, Sutka J, Galston AW. 1993. Chromosomal localization of osmotic and saltstress-induced differential alterations in polyamine contentin wheat. Plant Science, 92, 203-211

Gao L, Wang A, Li X, Dong K, Wang K, Appels R, Ma W, YanY. 2009. Wheat quality related differential expressionsof albumins and globulins revealed by two-dimensionaldifference gel electrophoresis (2-D DIGE). Journal ofProteomics, 3, 279-296

Kuspria J, Unrau J. 1957. Genetic analyses of certain charactersin common wheat using whole chromo some substitutionlines. Canadian Journal of Plant Science, 37, 300-326

Kocsy G, Szalai G, Sutka J, Paldi E, Galiba G. 2004. Heattolerance together with heat stress-induced changes inglutathione and hydroxymethylglutathione levels is affectedby chromosome 5A of wheat. Plant Science, 166, 451-458

Law C N, Snape J W, Worland A J. 1987. Aneuploidity in wheatand its uses in genetic analysis. In: Lupton F G H, ed.,Wheat Breeding. University Press, Cambridge. pp. 88-91

Mahmood A, Baenziger P S, Budak H, Gill K S, Dweikat I. 2004.The use of microsatellite markers for the detection of geneticsimilarity among winter bread wheat lines for chromosome3A. Theoretical and Applied Genetics, 109, 1494-1503

Payne P I. 1987. Genetics of wheat storage proteins and theeffect of allelic variation on bread-making quality. AnnualReview of Plant Physiology, 38, 141-153

Shah M M, Baenziger P S, Yen Y, Gill K S, Moreno-Sevilla B,Haliloglu K. 1999a. Genetic analysis of agronomic traitscontrolled by wheat chromosome 3A. Crop Science, 39,1016-1021

Shah M M, Gill K S, Baenziger P S, Yen Y, Kaep pler S M,Ariyarathne H M. 1999b. Molecular mapping of loci foragronomic traits on chromosome 3A of bread wheat. CropScience, 39, 1728-1732

Shewry P R. 2009. Wheat. Journal of Experimental Botany,60, 1537-1553

Somers D J, Isaac P, Edwards K. 2004. A high-densitymicrosatellite consensus map for bread wheat (Triticumaestivum L.). Theoretical and Applied Genetics, 109,1105-1114

Wang K, Lin Z S, Wang S L, Du L P, Li J R, Xu H J, Yan Y M,Ye X G. 2013. Development, identification, and geneticanalysis of a quantitative dwarfing somatic variation line inwheat (Triticum aestivum L.). Crop Science, 53, 1032-1041

Yan Y, Hsam S L K, Yu S L K, Jiang Y, Ohtsuka I, Zeller F J.2003. HMW and LMW gluten in alleles among putativetetraploid and hexaploid T. spelta progenitors. Theoreticaland Applied Genetics, 107, 1321-1330

Yang K, Chang X P, Hu R H, Jia J Z. 1998. Chromosomallocations of genes association with proline accumulationunder drought stress in wheat. Acta Agronomica Sinica,27, 363-366

Yen Y, Baenziger P S. 1992. A better way to constructrecombinant chromosome lines and their controls. Genome,35, 827-830

Yen Y, Baenziger P S. 1994. Wheat chromo some 2D carriesgenes controlling the activity of two DNA-degradingenzymes. Theoretical and Applied Genetics, 88, 30-32

Zemetra R S, Morris R. 1988. Effects of an intercultivaralchromosome substitution on winterhadriness andvernalization in wheat. Genetics, 119, 453-456

Zemetra R S, Morris R, Schmidt J W. 1986. Gene locations forheading date using reciprocal chromosome substitutions inwinter wheat. Crop Science, 26, 531-533

Zheng J F, Mi S Y, Jing J J, Bai Z Y, Li C D. 2013. Principalcomponent analysis and comprehensive evaluation onphysiological traits of tolerance to low phosphorus stressin wheat substitution. Scientia Agricultura Sinica, 46,1984–1993. (in Chinese)
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