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Journal of Integrative Agriculture  2023, Vol. 22 Issue (1): 41-51    DOI: 10.1016/j.jia.2022.08.049
Crop Science Advanced Online Publication | Current Issue | Archive | Adv Search |
TaABI19 positively regulates grain development in wheat
LIU Yun-chuan1, 2, WANG Xiao-lu1, 3, HAO Chen-yang1, IRSHAD Ahsan4, LI Tian1, LIU Hong-xia1, HOU Jian1, ZHANG Xue-yong1
1 National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2 State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, P.R.China
3 Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, P.R.China
4 National Key Facility for Crop Gene Resources and Genetic Improvement/National Center of Space Mutagenesis for Crop Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China

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摘要  淀粉是谷物胚乳中最重要的成分,其合成受到多种转录因子的调控。然而,这些转录因子在谷物之间是否具有保守功能尚不清楚。本研究依据玉米(Zea mays L.)的同源基因,克隆了属于一个B3家族的转录因子,命名为TaABI19。DNA和蛋白质序列比对分析,发现ABI19在玉米和小麦中具有保守性。TaABI19在小麦幼穗和发育的籽粒中高表达,编码一个核定位的转录激活子。EMS诱变获得的taabi19-b1突变体表现为淀粉合成下调、粒长变短和千粒重降低。此外,我们还发现TaABI19能够结合到TaPBF亚基因组同源基因的启动子上并增强其表达。单倍型关联表明,TaABI19-B1与千粒重显著相关。TaABI19-B1-Hap2-Hap3在中国小麦育种中被正向选择。在现代栽培品种中,TaABI19的优异单倍型比例不足50%,表明TaABI19仍可作为标记辅助选择育种的目标位点,提高我国小麦产量。

Abstract  Starch is the most important component in endosperm, and its synthesis is regulated by multiple transcription factors (TFs) in cereals. However, whether the functions of these TFs are conserved or not among cereals unclear yet. Here, we cloned a B3 family TF, named as TaABI19 based on its orthologous in maize (Zea mays L.). Alignment of DNA and protein showed that ABI19 was conserved in maize and wheat (Triticum aestivum L.). We found that TaABI19 was highly expressed in young spike and developing grains and encoded a nucleus-localized transcriptional activator in wheat. The taabi19-b1 null mutants obtained by EMS performed down-regulation of starch synthesis, shorter grain length and lower thousand grain weight (TGW). Furthermore, we provided TaABI19 could bind to the promoters of TaPBF homology genes and enhance their expression. Haplotype association showed that TaABI19-B1 was significantly associated with TGW. We found that Hap2 and Hap3 were favored and underwent positive selection in China wheat breeding. Less than fifty percent in the modern cultivars conveying favored haplotypes indicates TaABI19 still can be considered as target loci for marker-assisted selection breeding to increase TGW in China.
Keywords:  common wheat        transcription factor        haplotype        thousand grain weight  
Received: 20 August 2021   Online: 29 November 2021   Accepted:
Fund:  This work was supported by the the Central Public-interest Scientific Institution Basal Research Fund, Chinese Academy of Agricultural Sciences (Y2017PT39).

About author:  LIU Yun-chuan, E-mail:

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LIU Yun-chuan, WANG Xiao-lu, HAO Chen-yang, IRSHAD Ahsan, LI Tian, LIU Hong-xia, HOU Jian, ZHANG Xue-yong. 2023. TaABI19 positively regulates grain development in wheat. Journal of Integrative Agriculture, 22(1): 41-51.

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