|TaABI19 positively regulates grain development in wheat
|LIU Yun-chuan1, 2, WANG Xiao-lu1, 3, HAO
Chen-yang1, IRSHAD Ahsan4, LI Tian1, LIU
Hong-xia1, HOU Jian1, ZHANG Xue-yong1
|1 National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2 State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, P.R.China
3 Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, P.R.China
4 National Key Facility for Crop Gene Resources and Genetic Improvement/National Center of Space Mutagenesis for Crop Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
Abstract Starch is the most important component in endosperm, and its synthesis is
regulated by multiple transcription factors (TFs) in cereals. However, whether
the functions of these TFs are conserved or not among cereals unclear yet. Here,
we cloned a B3 family TF, named as TaABI19 based on its orthologous in
maize (Zea mays L.). Alignment of DNA and protein showed that ABI19 was conserved in maize and wheat (Triticum aestivum L.). We found that TaABI19 was highly expressed in
young spike and developing grains and encoded a nucleus-localized
transcriptional activator in wheat. The taabi19-b1 null mutants
obtained by EMS performed down-regulation of starch synthesis, shorter grain
length and lower thousand grain weight (TGW). Furthermore, we provided TaABI19
could bind to the promoters of TaPBF homology genes and enhance their
expression. Haplotype association showed that TaABI19-B1 was
significantly associated with TGW. We found that Hap2 and Hap3 were favored and underwent positive selection in China wheat breeding. Less than
fifty percent in the modern cultivars conveying favored haplotypes indicates TaABI19 still can be considered as target loci for marker-assisted
selection breeding to increase TGW in China.
Received: 20 August 2021
Online: 29 November 2021
|Fund: This work was supported by the the Central Public-interest Scientific Institution Basal Research Fund, Chinese Academy of Agricultural Sciences (Y2017PT39).
|About author: LIU Yun-chuan, E-mail: firstname.lastname@example.org
Cite this article:
LIU Yun-chuan, WANG Xiao-lu, HAO Chen-yang, IRSHAD Ahsan, LI Tian, LIU Hong-xia, HOU Jian, ZHANG Xue-yong.
TaABI19 positively regulates grain development in wheat. Journal of Integrative Agriculture, 22(1): 41-51.
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