Please wait a minute...
Journal of Integrative Agriculture
Advanced Online Publication | Current Issue | Archive | Adv Search
Genome wide linkage mapping for black point resistance in a recombinant inbred line population of Zhongmai 578 and Jimai 22
Tiantian Chen1, 2, Lei Li1, 2, Dan Liu1, Yubing Tian1, 2, Lingli Li1, 2, Jianqi Zeng1, 2, Awais Rasheed1, Shuanghe Cao1, 2, Xianchun Xia1, Zhonghu He1, 2, 3, Jindong Liu1, 2, Yong Zhang1, 2#

1 Institute of Crop Sciences, State Key Laboratory of Crop Gene Resources and Breeding, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China

2 Zhongyuan Research Center, Chinese Academy of Agricultural Sciences (CAAS), Xinxiang 453519, China

3 International Maize and Wheat Improvement Center (CIMMYT), China Office, c/o CAAS, Beijing 100081, China

Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
摘要  小麦黑胚病表现为籽粒胚部呈现明显的黑褐色斑点,会降低小麦品质和商品等级。挖掘抗性遗传位点可有效提升小麦品种黑胚病抗性。本研究以中麦578/济麦22重组自交系(Recombinant inbred line, RIL)的262份家系为研究材料,利用5个环境的黑胚率表型数据小麦50K SNP芯片构建的高密度遗传连锁图谱,定位到6个稳定的黑胚病抗性遗传位点(Quantitative trait loci, QTL),并命名为QBp.caas-2AQBp.caas-2B1QBp.caas-2B2QBp.caas-2DQBp.caas-3AQBp.caas-5B,分别解释2.1-28.8%的表型变异。其中,QBp.caas-2B1QBp.caas-2B2的抗性等位基因来自中麦578,其余位点抗性等位基因来自济麦22。在以上位点中,QBp.caas-2B2QBp.caas-2DQBp.caas-3A与之前报道的位点接近或一致,而QBp.caas-2AQBp.caas-2B1QBp.caas-5B可能是新的抗性位点。利用165份自然品种对5KASP标记(Kasp_2A_BPKasp_2B1_BPKasp_2B2_BPKasp_3A_BPKasp_5B_BP)的有效性进行了验证。本研究为小麦黑胚病分子标记辅助选择育种提供了可用QTL和分子标记。

Abstract  Black point, a black discoloration of the grain embryo, downgrades the grain quality and commodity grade.  Identification of the underlying genetic loci can facilitate the improvement of black point resistance in wheat.  Here, 262 recombinant inbred lines (RILs) from the cross of Zhongmai 578/Jimai 22 were evaluated for black point reaction in five environments.  A high-density genetic linkage map of the RIL population was constructed with the wheat 50K single nucleotide polymorphism (SNP) array.  Six stable QTLs for black point resistance, QBp.caas-2A, QBp.caas-2B1, QBp.caas-2B2QBp.caas-2D, QBp.caas-3A and QBp.caas-5B were detected, explaining 2.1-28.8% of the phenotypic variances.  The resistance alleles of QBp.caas-2B1 and QBp.caas-2B2 were contributed by Zhongmai 578 while the others were from Jimai 22.  QBp.caas-2B2, QBp.caas-2D and QBp.caas-3A are overlapped with previously reported loci, whereas QBp.caas-2AQBp.caas-2B1 and QBp.caas-5B are likely to be new.  Five KASP markers, Kasp_2A_BP, Kasp_2B1_BP, Kasp_2B2_BP, Kasp_3A_BP and Kasp_5B_BP were validated in a natural population of 165 cultivars.  The findings provide useful QTL and molecular markers for improvement of black point in wheat resistance in marker-assisted breeding.
Keywords:  black point       candidate gene              common wheat              Kompetitive allele-specific PCR              quantitative trait locus  
Online: 19 January 2024  
About author:  Tiantian Chen, E-mail: 2925018015@qq.com; Correspondence Jindong Liu, Tel: +86-10-82108889, E-mail: liujindong@caas.cn; Yong Zhang, Tel: +86-10-82108745, E-mail: zhangyong05@caas.cn

Cite this article: 

Tiantian Chen, Lei Li, Dan Liu, Yubing Tian, Lingli Li, Jianqi Zeng, Awais Rasheed, Shuanghe Cao, Xianchun Xia, Zhonghu He, Jindong Liu, Yong Zhang. 2024. Genome wide linkage mapping for black point resistance in a recombinant inbred line population of Zhongmai 578 and Jimai 22. Journal of Integrative Agriculture, Doi:10.1016/j.jia.2023.12.039

Al-Sadi A M. 2021. Bipolaris sorokiniana-induced black point, common root rot, and spot blotch diseases of wheat: A review. Frontiers in Cellular and Infection Microbiology, 11, 584899.

Bensassi F, Zid M, Rhouma A, Bacha H, Hajlaoui M R. 2009. First report of Alternaria species associated with black point of wheat in Tunisia. Annals of Microbiology, 59, 465-467.

Conner R L, Hwang S F, Stevens R R. 2009. Fusarium proliferatum: A new causal agent of black point in wheat. Canadian Journal of Plant Pathology, 18, 419-423.

Conner R L, Kuzyk A D. 1988. Effectiveness of fungicides in controlling stripe rust, leaf rust, and black point in soft white spring wheat. Canadian Journal of Plant Pathology, 10, 321-326.

Conner R L. 1989. Influence of irrigation and precipitation on incidence of black point in soft white spring wheat. Canadian Journal of Plant Pathology, 11, 388-392.

Desjardins A E, Busman M, Proctor R H, Stessman R. 2007. Wheat kernel black point and fumonisin contamination by Fusarium proliferatum. Food Additives and Contaminants, 24, 1131-1137.

Dexter J E, Matsuo R R. 1982. Effect of smudge and black point, mildewed kernels, and ergot on durum wheat quality. Cereal Chemistry59, 63-69.

Ellis S A, Gooding M J, Thompson A J. 1996. Factors influencing the relative susceptibility of wheat cultivars (Triticum aestivum L.) to black point. Crop Protection, 15, 69-76.

Fernandez M R, Conner R L. 2011. Black point and smudge in wheat. Prairie Soils Crops, 4, 158-164.

Fernandez M R, Sissons M, Conner R L, Wang H, Clarke J M. 2011. Influence of biotic and abiotic factors on dark discoloration of durum wheat kernels. Crop Science, 51, 1205-1214.

Fernandez M R, Wang H, Singh A K. 2014. Impact of seed discoloration on emergence and early plant growth of durum wheat at different soil gravimetric water contents. Canadian Journal of Plant Pathology36, 509-516.

Fuerst E P, Okubara P A, Anderson J V, Morris C F. 2014. Polyphenol oxidase as a biochemical seed defense mechanism. Frontiers in Plant Science, 5, 689.

Gao C, Song G L, Qu K F, Li M Y, Jiang Y M, Yin G H, Niu J S, Tang J W, Gao Y, Li Q Y. 2023. Quantitative trait loci for resistance to black point caused by Bipolaris sorokiniana in bread wheat. Molecular Breeding, 43, 10.

Ge H H, Wu Y, Xiao Y Z. 2011. Structure, catalytic mechanism and applications of laccases: A review. Chinese Journal of Biotechnology, 27, 156-163.

Janusz G, Pawlik A, Burek U Ś, Polak J, Sulej J, Wilkołazka A J, Paszczyński A. 2020. Laccase properties, physiological functions, and evolution. International Journal of Molecular Sciences, 21, 966.

Jolie R P, Duvetter T, Van Loey A M, Hendrickx M E. 2010. Pectin methylesterase and its proteinaceous inhibitor: A review. Carbohydrate Research, 18, 2583-2595.

Kahl S M, Ulrich A, Kirichenko A A, Müller M E. 2015. Phenotypic and phylogenetic segregation of Alternaria infectoria from small-spored Alternaria species isolated from wheat in Germany and Russia. Journal of Applied Microbiology, 119, 1637-1650.

Kumar J, Schäfer P, Hückelhoven R, Langen G, Baltruschat H, Stein E, Nagarajan S, Kogel K H. 2002. Bipolaris sorokiniana, a cereal pathogen of global concern: Cytological and molecular approaches towards better control. Molecular Plant Pathology, 3, 185-195.

Lehmensiek A, Campbell A W, Williamson P M, Michalowitz M, Sutherland M W, Daggard G. 2004. QTLs for black point resistance in wheat and the identification of potential markers for use in breeding programs. Plant Breeding, 123, 410-416.

Li H H, Ye G Y, Wang J K. 2007. A modified algorithm for the improvement of composite interval mapping. Genetics, 175, 361-374.

Li L L, Zhang Y J, Zhang Y, Li M, Xu D A, Tian X L, Song J, Luo X M, Xie L N, Wang D S, He Z H, Xia X C, Zhang Y, Cao S H. 2021a. Genome-wide linkage mapping for pre-harvest sprouting resistance in wheat using 15K single-nucleotide polymorphism arrays. Frontiers in Plant Science, 12, 1-9.

Li Q Y, Gao C, Xu K G, Jiang Y M, Niu J S, Yin G H, Wang C Y. 2021b. Transcriptome-based analysis of resistance mechanism to black point caused by Bipolaris sorokiniana in wheat. Scientific Reports, 11, 6911.

Li Q Y, Gao C, Zhang F F, Li Y J, Chen X G, Gao Y, Tang J W, Yin G H. 2022a. Advances in detection of resistance loci to black point disease in wheat. Journal of Henan Agricultural University, 56, 1-20. (in Chinese)

Li Q Y, Hu R Y, Guo Z F, Wang S Y, Gao C, Jiang Y M, Tang J W, Yin G H. 2022b. SNP-based identification of QTL for resistance to black point caused by Bipolaris sorokiniana in bread wheat. The Crop Journal, 10, 767-774.

Li Q Y, Niu H B, Xu K G, Xu Q Q, Wang S Y, Liang X L, Jiang Y M, Niu J S. 2020a. GWAS for resistance against black point caused by Bipolaris sorokiniana in wheat. Journal of Cereal Science, 91, 102859.

Li Q Y, Qin Z, Jiang Y M, Shen C C, Duan Z B, Niu J S. 2014. Screening wheat genotypes for resistance to black point and the effects of diseased kernels on seed germination. Journal of Plant Diseases and Protection, 121, 79-88.

Li Q Y, Wang S Y, Chang S W, Xu K G, Li M Y, Xu Q Q, Jiang Y M, Niu J S. 2019. Key periods and effects of meteorological factors affecting incidence of wheat black point in the Yellow and Huai wheat area of China. Crop Protection, 125, 104882.

Li Q Y, Xu K G, Wang S Y, Li M Y, Jiang Y M, Liang X L, Niu J S, Wang C Y. 2020b. Enzymatic browning in wheat kernels produces symptom of black point caused by Bipolaris sorokiniana. Frontiers in Microbiology, 11, 526266.

Liu D, Zhao D H, Zeng J Q, Shawai R S, Tong J Y, Li M, Li F J, Zhou S, Li H W, Xia X C, Tian Y B, Zhu Q, Wang C P, Wang D S, He Z H, Liu J D, Zhang Y. 2023. Identification of genetic loci for grain yield-related traits in the wheat population Zhongmai 578/Jimai 22. Journal of Integrative Agriculture, 22, 1985-1999.

Liu J D, He Z H, Rasheed A, Wen W E, Yan J, Zhang P Z, Wan Y X, Zhang Y, Xie CJ, Xia X C. 2017. Genome-wide association mapping of black point reaction in common wheat (Triticum aestivum L.). BMC Plant Biology, 17, 1-12.

Liu J D, He Z H, Wu L, Bai B, Wen W E, Xie C J, Xia X C. 2016. Genome-wide linkage mapping of QTL for black point reaction in bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 129, 2179-2190.

Logrieco A, Bottalico G, Mulé G, Moretti A, Perrone G. 2003. Epidemiology of toxigenic fungi and their associated mycotoxins for some Mediterranean crops. European Journal of Plant Pathology, 109, 645-667.

Lv G G, Dong Z D, Wang Y D, Geng J Y, Li J, Lv X L, Sun C W, Ren Y, Zhang J W, Chen F. 2020. Identification of genetic loci of black point in Chinese common wheat by genome-wide association study and linkage mapping. Plant Disease, 104, 2005-2013.

Mak Y, Willows R D, Roberts T H, Wrigley C W, Sharp P J, Copeland L E S. 2006. Black point is associated with reduced levels of stress, disease and defense related proteins in wheat grain. Molecular Plant Pathology, 7, 177-189.

March T J, Able J A, Schultz C, Able A J. 2007. A novel late embryogenesis abundant protein and peroxidase associated with black point in barley grains. Proteomics, 7, 3800-3808.

March T J, Able J A, Willsmore K, Schultz C J, Able A J. 2008. Comparative mapping of a QTL controlling black point formation in barley. Functional Plant Biology, 35, 427-437.

Masiello M, Somma S, Susca A, Ghionna V, Logrieco A F, Franzoni M, Ravaglia S, Meca G, Moretti A. 2020. Molecular identification and mycotoxin production by Alternaria species occurring on durum wheat, showing black point symptoms. Toxins, 12, 275.

Meng L, Li H, Zhang L, Wang J. 2015. QTL IciMapping, integrated software for genetic linkage map construction and quantitative trait locus mapping in biparental populations. The Crop Journal, 3, 269-283.

Nyquist W E, Baker R J. 1991. Estimation of heritability and prediction of selection response in plant populations. Critical Reviews in Plant Sciences, 10, 235-322.

Rasheed A, Hao Y F, Xia X C, Khan A, Xu Y, Varshney R K, He Z H. 2017. Crop breeding chips and genotyping platforms: Progress, challenges, and perspectives. Molecular Plant, 10, 1047-1064.

Régnier T, Macheix J J. 1996. Changes in wall bound phenolic acids, phenylalanine and tyrosine ammonia-lyases, and peroxidases in developing durum wheat grains (Triticum turgidum L. var. durum). Journal of Agricultural and Food Chemistry, 44, 1727-1730.

Semagn K, Babu R, Hearne S, Olsen M. 2014. Single nucleotide polymorphism genotyping using Kompetitive Allele Specific PCR (KASP): Overview of the technology and its application in crop improvement. Molecular Breeding, 33, 1-14.

Shawai R S, Liu D, Li L L, Chen T T, Li M, Cao S H, Xia X C, Liu J D, He Z H, Zhang Y. 2022. QTL mapping for pre-harvest sprouting in a recombinant inbred line population of elite wheat varieties Zhongmai 578 and Jimai 22. The Crop Journal, 11, 863-869.

Sissons M, Sissons S, Egan N. 2010. The black point status of selected tetraploid species and Australian durum wheat and breeding lines. Crop Science, 50, 1279-1286.

Somma S, Amatulli M T, Masiello M, Moretti A, Logrieco A F. 2019. Alternaria species associated to wheat black point identified through a multilocus sequence approach. International Journal of Food Microbiology, 293, 34-43.

Stam P. 1993. Construction of integrated genetic linkage maps by means of a new computer package: JoinMap. Plant Journal, 3, 739-744. 

Tang H, Tan Z, Wang X X, Yang L S, Chen G Y, Yu H, Pu Z E, Jiang Q T, Li M L, Chen M P, Qi P F, Li W, Liu Y J, Wang J R. 2022. Genome-wide association study of kernel black point resistance in Chinese wheat landraces. Plant Disease, 106, 1428-1433.

Voorrips R E. 2002. MapChart: Software for the graphical presentation of linkage maps and QTLs. Journal of Heredity, 93, 77-78.

Walker J R L, Ferrar P H. 1998. Diphenol oxidases, enzyme-catalysed browning and plant disease resistance. Biotechnology and Genetic Engineering Reviews, 15, 457-498.

Wang S, Wong D, Forrest K, Allen A, Chao S, Huang B E, Maccaferri M, Salvi S, Milner S G, Cattivelli L, Mastrangelo A M, Stephen S, Barker G, Wieseke R, Plieske J, Lillemo M, Mather D, Appels R, Dulferos R, Brown G G, et al. 2014. Characterization of polyploid wheat genomic diversity using the high-density 90,000 SNP array. Plant Biotechnology Journal, 12, 787-796.

Wang S Y, Li Q Y, Jiang Y M, Xu K G, Li M Y, Niu J S, Yan Y Z. 2021. Genetic analysis of resistance to black embryo disease in wheat Shannong 4143 and detection of resistance genetic loci. Phytopathology Research, 51, 225-235.

Wei J X, Geng H W, Zhang Y, Liu J D, Wen W E, Xia X C, Chen X M, He Z H. 2015. Mapping quantitative trait loci for peroxidase activity and developing gene-specific markers for TaPod-A1 on wheat chromosome 3AL. Theoretical and Applied Genetics, 128, 2067-2076.

Williamson P M. 1997. Black point of wheat: In vitro production of symptoms, enzymes involved, and association with Alternaria alternata. Crop and Pasture Science, 48, 13-20.

Wormit A, Usadel B. 2018. The multifaceted role of pectin methylesterase inhibitors (PMEIs). International Journal of Molecular Sciences, 19, 2878.

Yang L, Zhao D H, Meng Z L, Xu K J, Xia X C, Cao S H, Tian Y B, He Z H, Zhang Y. 2020. QTL mapping for grain yield-related traits in bread wheat via SNP-based selective genotyping. Theoretical and Applied Genetics, 133, 857-872.

Zhai S N, He Z H, Wen W E, Jin H, Liu J D, Zhang Y, Liu Z Y, Xia X C. 2016. Genome-wide linkage mapping of flour color-related traits and polyphenol oxidase activity in common wheat. Theoretical and Applied Genetics, 129, 377-394.

No related articles found!
No Suggested Reading articles found!