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Journal of Integrative Agriculture  2022, Vol. 21 Issue (7): 1867-1876    DOI: 10.1016/S2095-3119(21)63605-5
Special Issue: 麦类遗传育种合辑Triticeae Crops Genetics · Breeding · Germplasm Resources
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Influence of high-molecular-weight glutenin subunit deletions at the Glu-A1 and Glu-D1 loci on protein body development, protein components and dough properties of wheat (Triticum aestivum L.)
LIU Da-tong*, ZHANG Xiao*, JIANG Wei, LI Man, WU Xu-jiang, GAO De-rong, BIE Tong-de, LU Cheng-bin
Key Laboratory of Wheat Biology and Genetic Improvement for Low & Middle Yangtze Valley, Ministry of Agriculture and Rural Affairs/Lixiahe Institute of Agricultural Sciences of Jiangsu, Yangzhou 225007, P.R.China
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High-molecular-weight glutenin subunits (HMW-GSs) play a critical role in determining the viscoelastic properties of wheat.  As the organelle where proteins are stored, the development of protein bodies (PBs) reflects the status of protein synthesis and also affects grain quality to a great extent.  In this study, with special materials of four near-isogenic lines in a Yangmai 18 background we created, the effects of Glu-A1 and Glu-D1 loci deletions on the development and morphological properties of the protein body, protein components and dough properties were investigated.  The results showed that the deletion of the HMW-GS subunit delayed the development process of the PBs, and slowed the increases of volume and area of PBs from 10 days after anthesis (DAA) onwards.  In contrast, the areas of PBs at 25 DAA, the middle or late stage of endosperm development, showed no distinguishable differences among the four lines.  Compared to the wild type and single null type in Glu-A1, the ratios of HMW-GSs to low-molecular-weight glutenin subunits (LMW-GSs), glutenin macropolymer (GMP) content, mixograph parameters as well as extension parameters decreased in the single null type in Glu-D1 and double null type in Glu-A1 and Glu-D1, while the ratios of gliadins (Gli)/glutenins (Glu) in those types increased.  The absence of Glu-D1 subunits decreased both dough strength and extensibility significantly compared to the Glu-A1 deletion type.  These results provide a detailed description of the effect of HMW-GS deletion on PBs, protein traits and dough properties, and contribute to the utilization of Glu-D1 deletion germplasm in weak gluten wheat improvement for use in cookies, cakes and southern steamed bread in China and liquor processing. 
Keywords:  wheat        near-isogenic lines        HMW-GS        deletion        protein body        dough property  
Received: 20 October 2020   Accepted: 18 December 2020
Fund: This work was supported by the National Key Research and Development Program of China (2016YFD0100500), the Natural Science Foundation of Jiangsu Province, China (BK20160448) and the National Natural Science Foundation of China (32071999 and 31700163).
About author:  LIU Da-tong, Tel: +86-514-87303868, E-mail:; ZHANG Xiao, Tel: +86-514-87303868, E-mail:; Correspondence LU Cheng-bin, Tel: +86-514-87307821, E-mail: * These authors contributed equally to this study.

Cite this article: 

LIU Da-tong, ZHANG Xiao, JIANG Wei, LI Man, WU Xu-jiang, GAO De-rong, BIE Tong-de, LU Cheng-bin. 2022. Influence of high-molecular-weight glutenin subunit deletions at the Glu-A1 and Glu-D1 loci on protein body development, protein components and dough properties of wheat (Triticum aestivum L.). Journal of Integrative Agriculture, 21(7): 1867-1876.

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