Please wait a minute...
Journal of Integrative Agriculture  2018, Vol. 17 Issue (08): 1697-1705    DOI: 10.1016/S2095-3119(17)61861-6
Crop Science Advanced Online Publication | Current Issue | Archive | Adv Search |
Identification and genetic analysis of multiple P chromosomes of Agropyron cristatum in the background of common wheat
CHEN Hong-xin1, HAN Hai-ming1, LI Qing-feng1, 2, ZHANG Jin-peng1, LU Yu-qing1, YANG Xin-ming1, LI Xiu-quan1, LIU Wei-hua1, LI Li-hui1
1 National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2 College of Agronomy, Northwest A&F University, Yangling 712100, P.R.China
Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
Abstract  
Agropyron cristatum, a wild relative of common wheat (Triticum aestivum L.), provides many desirable genetic resources for wheat improvement, such as tolerance to cold, drought, and disease.  To transfer and utilize these desirable genes, in this study, two wheat-A. cristatum derivatives II-13 and II-23 were identified and analyzed.  We found that the number of root tip cell chromosomes was 44 in both II-13 and II-23, but there were four and six P genome chromosomes in II-13 and II-23, respectively, based on genomic in situ hybridization (GISH).  The chromosome configurations of II-13 and II-23 were both 2n=22II by the meiotic analysis of pollen mother cells (PMCs) at metaphase I, indicating that there were two and three pairs of P chromosomes in II-13 and II-23, respectively.  Notably, wheat chromosome 7D was absent in derivative line II-13 while II-23 lacked chromosomes 4B and 7A based on SSR analysis combining fluorescence in situ hybridization (FISH) analysis with pAs1 and pSc119.2 as probes.  Chromosomes 2P and 7P were detected in both II-13 and II-23.  Another pair of P genome chromosomes in II-23 was determined to be 4P based on expressed-sequences tags-sequence tagged sites (EST-STS) markers specific to A. cristatum and FISH with probes pAcTRT1 and pAcpCR2.  Overall, these results suggest that II-13 was a 7P (7D) substitution line with one pair of additional 2P chromosomes and II-23 was a multiple 4P (4B), 7P (7A) substitution line with one pair of additional 2P chromosomes.  Moreover, we obtained six alien disomic addition lines and five alien disomic substitution lines by backcrossing.  These new materials will allow desirable genes from A. cristatum to be used in common wheat.
 
Keywords:  common wheat        Agropyron cristatum        derivatives        in situ hybridization        molecular markers  
Received: 01 August 2017   Accepted:
Fund: This work was supported by the National Key Research and Development Program of China (2016YFD0100102).
Corresponding Authors:  Correspondence LI Li-hui, E-mail: lilihui@caas.cn; LIU Wei-hua, E-mail: liuweihua@caas.cn   
About author:  CHEN Hong-xin, E-mail: 13051525421@126.com;

Cite this article: 

CHEN Hong-xin, HAN Hai-ming, LI Qing-feng, ZHANG Jin-peng, LU Yu-qing, YANG Xin-ming, LI Xiuquan, LIU Wei-hua, LI Li-hui. 2018. Identification and genetic analysis of multiple P chromosomes of Agropyron cristatum in the background of common wheat. Journal of Integrative Agriculture, 17(08): 1697-1705.

Alkhimova A G, Heslopharrison J S, Shchapova A I, Vershinin A V. 1999. Rye chromosome variability in wheat-rye additon and substitution lines. Chromosome Research, 7, 205–212.
Chen P D, Liu W X, Yuan J H, Wang X E, Zhou B, Wang S L, Zhang S Z, Feng Y G, Yang B J, Liu G X, Liu D J, Qi L L, Zhang P, Friebe B, Gill B S. 2005. Development and characterization of wheat-Leymus racemosus translocation lines with resistance to Fusarium Head Blight. Theoretical and Applied Genetics, 111, 941–948.
Chen P D, Zhou B, Qi L L, Liu D J. 1994. Identification of wheat-Haynaldia villosa amphiploid, addition, substitution and translocation lines by in situ hybridization using biotin-labelled genomic DNA as a probe. Acta Genetica Sinica, 22, 380–386.
Cifuentes M, Benavente E. 2009. Wheat-alien metaphase I pairing of individual wheat genomes and D genome chromosomes in interspecific hybrids between Triticum aestivum L. and Aegilops geniculata Roth. Theoretical and Applied Genetics, 119, 805–813.
Cuadrado A, Schwarzacher T, Jouve N. 2000. Identification of different chromatin classes in wheat using in situ hybridization with simple sequence repeat oligonucleotides. Theoretical and Applied Genetics, 101, 711–717.
Fu S L, Lv Z L, Qi B, Guo X, Li J, Liu B, Han F P. 2012. Molecular cytogenetic characterization of wheat-Thinopyrum elongatum addition, substitution and translocation lines with a novel source of resistance to wheat fusarium head blight. Journal of Genetics and Genomics, 39, 103–110.
Gill K S, Lubbers E L, Gill B S, Raupp W J, Cox T S. 1991. A Genetic Linkage map of Triticum tauschii (DD) and its relationship to the D genome of bread wheat (AABBDD). Genome, 34, 362–374.
Han F P, Lamb J C, Birchler J A. 2006. High frequency of centromere inactivation resulting in stable dicentric chromosomes of maize. Proceedings of the National Academy of Sciences of the United States of America, 103, 3238–3243.
Han H M, Bai L, Su J J, Zhang J P, Song L Q, Gao A N, Yang X M, Li X Q, Liu W H, Li L H. 2014. Genetic rearrangements of six wheat-Agropyron cristatum 6P addition lines revealed by molecular markers. PLoS ONE, 9, e91066.
Han H M, Liu W H, Lu Y Q, Zhang J P, Yang X M, Li X Q, Hu Z M, Li L H. 2017. Isolation and application of P genome-specific DNA sequences of Agropyron Gaertn in Triticeae. Planta, 245, 425–437.
Hu L J, Liu C, Zeng Z X, Li G R, Song X J, Yang Z J. 2012. Genomic rearrangement between wheat and Thinopyrum elongatum revealed by mapped functional molecular markers. Genes & Genomics, 34, 67–75.
Kato A, Lamb J C, Birchler J A. 2004. Chromosome painting using repetitive DNA sequences as probes for somatic chromosome identification in maize. Proceedings of the National Academy of Sciences of the United States of America, 101, 13554–13559.
Kishii M, Yamada T, Sasakuma T, Tsujimoto H. 2004. Production of wheat-Leymus racemosus chromosome addition lines. Theoretical and Applied Genetics, 109, 255–260.
Kong F, Wang H Y, Cao A Z, Qin B, Ji J H, Wang S L, Wang X E. 2008. Characterization of T. aestivum-H. californicum chromosome addition lines DA2H and MA5H. Journal of Genetics and Genomics, 35, 673–678.
Kumar A, Garg M, Kaur N, Chunduri V, Sharma S, Misser S, Kumar A, Tsujimoto H, Dou Q W, Gupta R K. 2017. Rapid development and characterization of chromosome specific translocation line of Thinopyrum elongatum with improved dough strength. Frontiers in Plant Science, 8, 1593.
Li H H, Lv M J, Song L Q, Zhang J P, Gao A N, Li L H, Liu W H. 2016. Production and identification of wheat-Agropyron cristatum 2P translocation lines. PLoS ONE, 11, e0145928.
Li H H, Jiang B, Wang J C, Lu Y Q, Zhang J P, Pan C L, Yang X M, Li X Q, Liu W H, Li L H. 2017. Mapping of novel powdery mildew resistance gene(s) from Agropyron cristatum chromosome 2P. Theoretical and Applied Genetics, 130, 109–121.
Li L H, Li X Q, Li P, Dong Y C, Zhao G S. 1997. Establishment of wheat-Agropyron cristatum alien addition lines. I. cytology of F3, F2BC1, BC4, and BC3F1 progenies. Acta Genetica Sinica, 24, 154–159.
Li Q F, Lu Y Q, Pan C L, Zhang J P, Liu W H, Yang X M, Li X Q, Xi Y J, Li L H. 2016. Characterization of a novel wheat-Agropyron cristatum 2P disomic addition line with powdery mildew resistance. Crop Science, 56, 2390.
Li Z, Ren Z L, Tan F Q, Tang Z X, Fu S L, Yan B J, Ren T H. 2016. Molecular cytogenetic characterization of new wheat-Rye 1R(1B) substitution and translocation lines from a Chinese Secale cereal L. Aigan with resistance to stripe rust. PLoS ONE, 11, e0163642.
Liu W H, Luan Y, Wang J C, Wang X G, Su J J, Zhang J P, Yang X M, Gao A N, Li L H. 2010. Production and identification of wheat-Agropyron cristatum (1.4P) alien translocation lines. Genome, 53, 472.
Lv M J, Lu Y Q, Li H H, Pan C L, Guo Y, Zhang J P, Yang X M, Li X Q, Liu W H, Li L H. 2016. Transferring desirable genes from Agropyron cristatum 7P chromosome into common wheat. PLoS ONE, 11, e0159577.
McArthur R I, Zhu X, Oliver R E, Klindworth D L, Xu S S, Stack R W, Wang R R, Cai X. 2012. Homoeology of Thinopyrum junceum and Elymus rectisetus chromosomes to wheat and disease resistance conferred by the Thinopyrum and Elymus chromosomes in wheat. Chromosome Research, 20, 699–715.
Nguyen V, Fleury D, Timmins A, Laga H, Hayden M, Mather D, Okada T. 2015. Addition of rye chromosome 4R to wheat increases anther length and pollen grain number. Theoretical and Applied Genetics, 128, 953–964.
Pan C L, Li Q F, Lu Y Q, Zhang J P, Yang X M, Li X Q, Li L H, Liu W H. 2017. Chromosomal localization of genes conferring desirable agronomic traits from Agropyron cristatum chromosome 1P. PLoS ONE, 12, e0175265.
Pu J, Wang Q, Shen Y F, Zhuang L F, Li C X, Tan M F, Bie T D, Chu C G, Qi Z J. 2015. Physical mapping of chromosome 4J of Thinopyrum bessarabicum using gamma radiation-induced aberrations. Theoretical and Applied Genetics, 128, 1319–1328.
Schneider A, Linc G, Molnár I, Molnár-Láng M. 2005. Molecular cytogenetic characterization of Aegilops biuncialis and its use for the identification of 5 derived wheat-Aegilops biuncialis disomic addition lines. Genome, 48, 1070–1082.
Tester M, Langridge P. 2010. Breeding technologies to increase crop production in a changing world. Science, 327, 818–822.
Wang R R, Larson S R, Jensen K B. 2010. Analyses of Thinopyrum bessarabicum, T. elongatum, and T. junceum chromosomes using EST-SSR markers. Genome, 53, 1083–1089.
Wu J, Yang X M, Wang H, Li H J, Li L H, Li X Q, Liu W H. 2006. The introgression of chromosome 6P specifying for increased numbers of florets and kernels from Agropyron cristatum into wheat. Theoretical and Applied Genetics, 114, 13–20.
Zhang J P, Liu W H, Han H M, Song L Q, Bai L, Gao Z H, Zhang Y, Yang X M, Li X Q, Gao A N, Li L H. 2015. De novo transcriptome sequencing of Agropyron cristatum to identify available gene resources for the enhancement of wheat. Genomics, 106, 129–136.
Zhang J P, Liu W H, Lu Y Q, Liu Q X, Yang X M, Li X Q, Li L H. 2017. A resource of large-scale molecular markers for monitoring Agropyron cristatum chromatin introgression in wheat background based on transcriptome sequences. Scientific Reports, 7, 11942.
Zhang R Q, Zhang M Y, Wang X E, Chen P D. 2014. Introduction of chromosome segment carrying the seed storage protein genes from chromosome 1V of Dasypyrum villosum showed positive effect on bread-making quality of common wheat. Theoretical and Applied Genetics, 127, 523–533.
 
[1] LI Jiao-jiao, ZHAO Li, LÜ Bo-ya, FU Yu, ZHANG Shu-fa, LIU Shu-hui, YANG Qun-hui, WU Jun, LI Jia-chuang, CHEN Xin-hong. Development and characterization of a novel common wheat–Mexico Rye T1DL·1RS translocation line with stripe rust and powdery mildew resistance[J]. >Journal of Integrative Agriculture, 2023, 22(5): 1291-1307.
[2] JIANG Yun, WANG De-li, HAO Ming, ZHANG Jie, LIU Deng-cai.

Development and characterization of wheat–Aegilops kotschyi 1Uk(1A) substitution line with positive dough quality parameters [J]. >Journal of Integrative Agriculture, 2023, 22(4): 999-1008.

[3] LIU Yun-chuan, WANG Xiao-lu, HAO Chen-yang, IRSHAD Ahsan, LI Tian, LIU Hong-xia, HOU Jian, ZHANG Xue-yong. TaABI19 positively regulates grain development in wheat[J]. >Journal of Integrative Agriculture, 2023, 22(1): 41-51.
[4] LIU Lei, WANG Heng-bo, LI Yi-han, CHEN Shu-qi, WU Ming-xing, DOU Mei-jie, QI Yi-yin, FANG Jing-ping, ZHANG Ji-sen. Genome-wide development of interspecific microsatellite markers for Saccharum officinarum and Saccharum spontaneum[J]. >Journal of Integrative Agriculture, 2022, 21(11): 3230-3244.
[5] WU Tian-ci, ZHU Xiu-liang, LÜ Liang-jie, CHEN Xi-yong, XU Gang-biao, ZHANG Zeng-yan. The wheat receptor-like cytoplasmic kinase TaRLCK1B is required for host immune response to the necrotrophic pathogen Rhizoctonia cerealis[J]. >Journal of Integrative Agriculture, 2020, 19(11): 2616-2627.
[6] SUN Hao-jie, SONG Jing-jing, XIAO Jin, XU Tao, WEI Xing, YUAN Chun-xia, CAO Ai-zhong, XING Liping, WANG Hai-yan, WANG Xiu-e. Development of EST-PCR markers specific to the long arm of chromosome 6V of Dasypyrum villosum[J]. >Journal of Integrative Agriculture, 2018, 17(08): 1720-1726.
[7] Syed Adeel Zafar, Amjad Hameed, Muhammad Amjad Nawaz, MA Wei, Mehmood Ali Noor, Muzammil Hussain, Mehboob-ur-Rahman. Mechanisms and molecular approaches for heat tolerance in rice (Oryza sativa L.) under climate change scenario[J]. >Journal of Integrative Agriculture, 2018, 17(04): 726-738.
[8] Ghulam Shabir, Kashif Aslam, Abdul Rehman Khan, Muhammad Shahid, Hamid Manzoor, Sibgha Noreen, Mueen Alam Khan, Muhammad Baber, Muhammad Sabar, Shahid Masood Shah, Muhammad Arif. Rice molecular markers and genetic mapping: Current status and prospects[J]. >Journal of Integrative Agriculture, 2017, 16(09): 1879-1891.
[9] LIU Guo-xiang, ZHANG Li-chao, XIA Chuan, JIA Ji-zeng, ZHANG Jun-cheng, ZHANG Qiang, DONG Chun-hao, KONG Xiu-ying, LIU Xu. Mapping of the heading date gene HdAey2280 in Aegilops tauschii[J]. >Journal of Integrative Agriculture, 2016, 15(12): 2719-2725.
No Suggested Reading articles found!