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| Determining N supplied sources and N use efficiency for peanut under applications of four forms of N fertilizers labeled by isotope 15N |
| WANG Cai-bin, ZHENG Yong-mei, SHEN Pu, ZHENG Ya-ping, WU Zheng-feng, SUN Xue-wu, YU Tian-yi, FENG Hao |
| Shandong Peanut Research Institute, Qingdao 266100, P.R.China |
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摘要 Rational application of different forms of nitrogen (N) fertilizer for peanut (Arachis hypogaea L.) requires tracking the N supplied sources which are commonly not available in the differences among the three sources: root nodule, soil and fertilizer. In this study, two kinds of peanut plants (nodulated variety (Huayu 22) and non-nodulated variety (NN-1)) were choosed and four kinds of N fertilizers: urea-N (CONH2-N), ammonium-N (NH4 +-N), nitrate-N (NO3 –-N) and NH4 ++NO3 –-N labeled by 15N isotope were applied in the field barrel experiment in Chengyang Experimental Station, Shandong Province, China, to determine the N supplied sources and N use efficiency over peanut growing stages. The results showed that intensities and amounts of N supply from the three sources were all higher at middle growing stages (pegging phase and podding phase). The accumulated amounts of N supply from root nodule, soil and fertilizer over the growing stages were 8.3, 5.3 and 3.8 g m–2 in CONH2-N treatment, which are all significantly higher than in the other three treatments. At seedling phase, soil supplied the most N for peanut growth, then root nodule controlled the N supply at pegging phase and podding phase, but soil mainly provided N again at the last stage (pod filling phase). For the whole growing stages, root nodule supplied the most N (47.8 and 43.0%) in CONH2-N and NH4 +-N treatments, whereas soil supplied the most N (41.7 and 40.9%) in NH4 ++ NO3 –-N and NO3 –-N treatments. The N use efficiency was higher at pegging phase and podding phase, while accumulated N use efficiency over the growing stages was higher in CONH2-N treatment (42.2%) than in other three treatments (30.4% in NH4 +-N treatment, 29.4% in NO3 –-N treatment, 29.4% in NH4 ++NO3 –-N treatment). In peanut growing field, application of CONH2-N is a better way to increase the supply of N from root nodule and improve the N use efficiency.
Abstract Rational application of different forms of nitrogen (N) fertilizer for peanut (Arachis hypogaea L.) requires tracking the N supplied sources which are commonly not available in the differences among the three sources: root nodule, soil and fertilizer. In this study, two kinds of peanut plants (nodulated variety (Huayu 22) and non-nodulated variety (NN-1)) were choosed and four kinds of N fertilizers: urea-N (CONH2-N), ammonium-N (NH4 +-N), nitrate-N (NO3 –-N) and NH4 ++NO3 –-N labeled by 15N isotope were applied in the field barrel experiment in Chengyang Experimental Station, Shandong Province, China, to determine the N supplied sources and N use efficiency over peanut growing stages. The results showed that intensities and amounts of N supply from the three sources were all higher at middle growing stages (pegging phase and podding phase). The accumulated amounts of N supply from root nodule, soil and fertilizer over the growing stages were 8.3, 5.3 and 3.8 g m–2 in CONH2-N treatment, which are all significantly higher than in the other three treatments. At seedling phase, soil supplied the most N for peanut growth, then root nodule controlled the N supply at pegging phase and podding phase, but soil mainly provided N again at the last stage (pod filling phase). For the whole growing stages, root nodule supplied the most N (47.8 and 43.0%) in CONH2-N and NH4 +-N treatments, whereas soil supplied the most N (41.7 and 40.9%) in NH4 ++ NO3 –-N and NO3 –-N treatments. The N use efficiency was higher at pegging phase and podding phase, while accumulated N use efficiency over the growing stages was higher in CONH2-N treatment (42.2%) than in other three treatments (30.4% in NH4 +-N treatment, 29.4% in NO3 –-N treatment, 29.4% in NH4 ++NO3 –-N treatment). In peanut growing field, application of CONH2-N is a better way to increase the supply of N from root nodule and improve the N use efficiency.
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Received: 30 September 2014
Accepted:
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| Fund: This work was supported by the Youth Scientific Research Foundation of Shandong Academy of Agricultural Sciences, China (2014QNM27), the Applying Basic Research Project of Qingdao, Shandong Province, China (14-2-4-90-jch), the Modern Agricultural Industry Technology System, China (SDAIT-05-021-04), the National Key Technology R&D Program of China (2014BAD11B04), the Key Innovation of Science and Technology Project of Shandong Academy of Agricultural Sciences, China (2014CXZ06-2; 2014CXZ11-2). |
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Corresponding Authors:
ZHENG Yaping,Tel: +86-532-87632130, Fax: +86-532-87626832,E-mail: ypzheng62@126.com.
E-mail: ypzheng62@126.com.
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| About author: WANG Cai-bin, E-mail: caibinw@126.com; ZHENG Yong-mei,E-mail: ymzhengrice@163.com;* These authors contributed equally to this study. |
Cite this article:
WANG Cai-bin, ZHENG Yong-mei, SHEN Pu, ZHENG Ya-ping, WU Zheng-feng, SUN Xue-wu, YU Tian-yi, FENG Hao.
2016.
Determining N supplied sources and N use efficiency for peanut under applications of four forms of N fertilizers labeled by isotope 15N. Journal of Integrative Agriculture, 15(2): 432-439.
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| Basu M, Bhadoria P B S, Mahapatra S C. 2008. Growth,nitrogen fixation, yield and kernel quality of peanut inresponse to lime, organic and inorganic fertilizer levels.Bioresource Technology, 99, 4675-4683Bollman M I, Vessey J K 2006. Differential effects of nitrateand ammonium supply on nodule initiation, development,and distribution on roots of pea (Pisum sativum). CanadianJournal of Botany, 84, 893-903 Caballero-Mellado J, Martinez-Romero E. 1999. Soil fertilizationlimits the genetic diversity of Rhizobium in bean nodules.Symbiosis, 26, 111-121Cookson P, Goh K M, Swift P S, Steele K W. 1990. Effect offrequency of application of 15N tracer and rate of fertilizernitrogen applied on estimation of nitrogen fixation by anestablished mixed grass/clover pasture using 15N enrichedtechnique. New Zealand Journal of Agricultural Research,33, 271-276Daimon H, Yoshioka M. 2000. Responses of root noduleformation and nitrogen fixation activity to nitrate in a splitrootsystem in peanut (Arachis hypogaea L.). Agronomyand Crop Science, 187, 89-95Divito G A, Sadras V O. 2014. How do phosphorus, potassiumand sulphur affect plant growth and biological nitrogenfixation in crop and pasture legumes? A meta-analysis.Field Crops Research, 156, 161-171Dong L, Li Y, Wu J, Huang M. 2008. Effects of different nitrogenforms on soil nitrogen supplying capacity and nitrogencontent of wheat plant and grain. Henan AgriculturalScience, 8, 81-83 (in Chinese)Findenegg G R. 1987. A comparative study of ammoniumtoxicity at different constant pH of the nutrient solution. Plantand Soil, 103, 239-243Fujikake H, Yamazaki A, Ohtake N, Sueyoshi K, MatsuhashiS, Ito T, Mizuniwa C, Kume T, Hashimoto S, Ishioka N S,Watanabe S, Osa A, Sekine T, Uchida H, Tsuji A, OhyamaT. 2003. Quick and reversible inhibition of soybean nodulegrowth by nitrate involves a decrease in sucrose supply tonodules. Journal of Experimental Botany, 54, 1379-1388Gan Y, Stulen I, van Keulen H, Kuiper P J C. 2004. Lowconcentrations of nitrate and ammonium stimulate nodulationand N2 fixation while inhibiting specific nodulation (noduleDW g-1 root dry weight) and specific N2 fixation (N2 fixed g−1root dry weight) in soybean Plant and Soil, 258, 281-292Goh K M, Mansur I, Mead D J, Sweet G B. 1996. Biologicalnitrogen fixing capacity and biomass production of differentunderstorey pastures in a Pinus radiate-pasture agroforestrysystem in New Zealand. Agroforestry Systems, 34, 33-39Kronzucker H J, Kirk G J D, Siddiqi M Y, Glass A D M. 1998.Effects of hypoxia on 13NH4+ fluxes in rice roots. PlantPhysiology, 116, 581-587Linquist B A, Liu L, van Kessel C, van Groenigen K J. 2013.Enhanced efficiency nitrogen fertilizers for rice systems:Meta-analysis of yield and nitrogen uptake. Field CropsResearch, 154, 246-254Liu Y, Wu L, Baddeley J A, Watson C A. 2011. Models ofbiological nitrogen fixation of legumes. A review. Agronomyfor Sustainable Development, 31, 155-172Lu J K, Kang L H, Sprent J I, Xu D P, He X H. 2003. Two-waytransfer of nitrogen between Dalbergia odorifera and itshemiparasite Santalum album is enhanced when the hostis effectively nodulated and fixing nitrogen. Tree Physiology,33, 464-474Lü D, Yan B, Wang L, Deng Z, Zhang Y. 2013. Changes inphosphorus fractions and nitrogen forms during compostingof pig manure with rice straw. Journal of IntegrativeAgriculture, 12, 1855-1864Murphy D V, Recous S, Stockdale E A, Fillery I R P, Jensen LS, Hatch D J, Goulding K W T. 2003. Gross nitrogen fluxesin soil: Theory, measurement and application of 15N pooldilution techniques. Advances in Agronomy, 79, 69-118Rai R. 1992. Effect of nitrogen levels and Rhizobium strains onsymbiotic N2 fixation and grain yield of Phaseolus vulgarisL. genotypes in normal and saline-sodic soils. Biology andFertility of Soils, 14, 293-299Sulieman S, Schulze J, Tran L S P. 2014. N-feedback regulationis synchronized with nodule carbon alteration in Medicagotruncatula under excessive nitrate or low phosphorusconditions. Journal of Plant Physiology, 171, 407-410Sun H, Li S, Wang Y, Wang M. 2010. Effects of nitrogenapplication on source of nitrogen accumulation and yieldsof different peanut cultivars. Plant Nutrition and FertilizerScience, 16, 153-157 (in Chinese)Svenning M M, Junttila O, Macduff J H. 1996. Differential ratesof inhibition of N2 fixation by sustained low concentrations ofNH4+ and NO3- in northern ecotypes of white clover (Trifoliumrepens L.). Journal of Experimental Botany, 47, 729-738Uchida Y, Akiyama H. 2013. Mitigation of postharvest nitrousoxide emissions from soybean ecosystems: A review. SoilScience and Plant Nutrition, 59, 477-487Wan S, Feng H, Zuo X, Zhang J. 2000. Effect of different levelsof nitrogen fertilizer on nitrogen use efficiency in peanut.Shandong Agricultural Science, 1, 31-33 (in Chinese)Wang C, Wan S. 2011. Physiology and Ecology of Peanut.China Agriculture Press, China. (in Chinese)Xiong H, Shen H, Zhang L, Zhang Y, Guo X, Wang P, Duan P, JiC, Zhong L, Zhang F, Zuo Y. 2013. Comparative proteomicanalysis for assessment of the ecological significance ofmaize and peanut intercropping. Journal of Proteomics,78, 447-460Yang G, Chu K, Tang H, Nie Y, Zhang X. 2013. 15N accumulation,recovery and distribution in cotton plant as affected by N rateand split. Journal of Integrative Agriculture, 12, 1855-1864Yu C, Xie Y, Hou J, Fu Y, Shen H, Liao X. 2014. Response ofnitrate metabolism in seedlings of oilseed rape (Brassicanapus L.) to low oxygen stress. Journal of IntegrativeAgriculture, 13, 2416-2423Zahran H H. 1999. Rhizobium-legume symbiosis and nitrogenfixation under severe conditions and in an arid climate.Microbiology and Molecular Biology Reviews, 63, 968-989Zhang M, Sun W, Liu Y, Luo S, Zhao J, Wu Q, Wu Z , Jiang Y.2014. Timing of N application affects net primary productionof soybean with different planting densities. Journal ofIntegrative Agriculture, 13, 2778-2787Zhang X, Zhang X, Zhang Y, Mao J, Li G, Zhao L. 2012.Effects of nitrogen application rate on nodulation, nitrogenabsorption and utilization of peanut. Journal of PeanutScience, 41, 12-17 (in Chinese) |
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