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Journal of Integrative Agriculture  2022, Vol. 21 Issue (11): 3185-3198    DOI: 10.1016/j.jia.2022.07.057
Crop Science Advanced Online Publication | Current Issue | Archive | Adv Search |
dep1 improves rice grain yield and nitrogen use efficiency simultaneously by enhancing nitrogen and dry matter translocation
HUANG Li-ying2, 3, Li Xiao-xiao1, ZHANG Yun-bo3, 4, Shah FAHAD5, WANG Fei1, 4

1 National Key Laboratory of Crop Genetic Improvement, MARA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, P.R.China

2 Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education, Yangtze University, Jingzhou 434025, P.R.China

3 College of Agriculture, Yangtze University, Jingzhou 434025, P.R.China

4 Hubei Collaborative Innovation Center for Grain Industry, Yangtze University, Jingzhou 434025, P.R.China

5 Department of Agronomy, The University of Haripur, Haripur 22620, Pakistan.

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摘要  携带dep1基因(直立密穗)的水稻品种具有高产和高氮利用效率的潜力。然而,在田间条件下,对这些品种高产和高氮利用效率有关的农艺和生理性状研究的较少。因此,本研究在0和120 kg N ha-1下对遗传背景均为南粳6号的两个分别携带DEP1 (NIL-DEP1) 和dep1-1 (NIL-dep1)基因的近等基因系材料(NILs)进行了产量和氮利用效率评价。综合所有的氮肥处理和种植年份,NIL-dep1的产量和氮素籽粒生产效率(NUEg)分别比NIL-DEP1高25.5%和21.9%。NIL-dep1相对于NIL-DEP1的产量优势主要源于较大的库容(即较高的总颖花数)、较高的结实率、总干物质积累和收获指数。氮素利用而非氮素吸收更有利于NIL-dep1的高产。NIL-dep1显著较高的NUEg与其较高的氮和干物质转运效率、较低的成熟期叶片和茎秆氮素浓度以及较高的叶片谷氨酰胺合成酶(GS)活性有关。综上,在大田条件下,dep1通过提高籽粒灌浆期的叶片GS活性增加氮素和干物质转运,进而提高水稻产量和氮利用效率。


The rice cultivars carrying dep1 (dense and erect panicle 1) have the potential to achieve both high grain yield and high nitrogen use efficiency (NUE).  However, few studies have focused on the agronomic and physiological performance of those cultivars associated with high yield and high NUE under field conditions.  Therefore, we evaluated the yield performance and NUE of two near-isogenic lines (NILs) carrying DEP1 (NIL-DEP1) and dep1-1 (NIL-dep1) genes under the Nanjing 6 background at 0 and 120 kg N ha–1.  Grain yield and NUE for grain production (NUEg) were 25.5 and 21.9% higher in NIL-dep1 compared to NIL-DEP1 averaged across N treatments and planting years, respectively.  The yield advantage of NIL-dep1 over NIL-DEP1 was mainly due to larger sink size (i.e., higher total spikelet number), grain-filling percentage, total dry matter production, and harvest index.  N utilization rather than N uptake contributed to the high yield of NIL-dep1.  Significantly higher NUEg in NIL-dep1 was associated with higher N and dry matter translocation efficiency, lower leaf and stem N concentration at maturity, and higher glutamine synthetase (GS) activity in leaves.  In conclusion, dep1 improved grain yield and NUE by increasing N and dry matter transport due to higher leaf GS activity under field conditions during the grain-filling period.

Keywords:  dep1        grain yield        nitrogen use efficiency        nitrogen translocation        rice  
Received: 18 April 2021   Accepted: 07 July 2021
Fund: This work was supported by the National Natural Science Foundation of China (32071948), the Fundamental Research Funds for the Central Universities, China (2662020ZKPY015), and the Guangxi Natural Science Foundation, China (2022GXNSFAA035488).  
About author:  HUANG Li-ying, E-mail: lyhuang8901@126; Correspondence WANG Fei, Tel: +86-27-87284385, E-mail:

Cite this article: 

HUANG Li-ying, Li Xiao-xiao, ZHANG Yun-bo, Shah FAHAD, WANG Fei. 2022. dep1 improves rice grain yield and nitrogen use efficiency simultaneously by enhancing nitrogen and dry matter translocation. Journal of Integrative Agriculture, 21(11): 3185-3198.

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